Electron Capture Dissociation for Structural Characterization of Multiply Charged Protein Cations
Zubarev R, Horn D, Fridriksson E, Kelleher N, Kruger N, Lewis M, Carpenter B, McLafferty F. Electron Capture Dissociation for Structural Characterization of Multiply Charged Protein Cations. Analytical Chemistry 2000, 72: 563-573. PMID: 10695143, DOI: 10.1021/ac990811p.Peer-Reviewed Original ResearchConceptsConventional MS/MS methodsElectron capture dissociationEven-electron ionsIon conversion efficiencyMS/MS methodCapture cross sectionCapture dissociationProtein cationsFragment ionsCAD spectraKinetic energy differenceStructural characterizationElectron captureEV differenceBackbone bondsIon cellsIonic chargeSecondary dissociationPotential wellMS methodCross sectionsIonsElectronsConversion efficiencyEnergy differenceElectron Capture Dissociation Produces Many More Protein Backbone Cleavages Than Collisional and IR Excitation
Zubarev R, Fridriksson E, Horn D, Kelleher N, Kruger N, Lewis M, Carpenter B, Mclafferty F. Electron Capture Dissociation Produces Many More Protein Backbone Cleavages Than Collisional and IR Excitation. 2000, 111-120. DOI: 10.1007/978-1-59259-719-2_7.Peer-Reviewed Original ResearchElectron capture dissociationCapture dissociationProtein backbone cleavageProtein cationsLabile HBackbone cleavageMolecular ionsReduction stepY ionsECD spectraIR excitationCleavage mechanismActivation methodRecombination energyLow-energy electronsInfrared irradiationElectron captureIonsSubsequent cleavageY cleavageConventional activation methodDissociationCleavageSmall proteinsCleavage products