2024
Lymphotoxin
Ruddle N. Lymphotoxin. 2024 DOI: 10.1016/b978-0-128-24465-4.00033-8.Peer-Reviewed Original Research
2010
A yeast‐based recombinogenic targeting toolset for transgenic analysis of human disease genes
Bentley KL, Shashikant CS, Wang W, Ruddle NH, Ruddle FH. A yeast‐based recombinogenic targeting toolset for transgenic analysis of human disease genes. Annals Of The New York Academy Of Sciences 2010, 1207: e58-e68. PMID: 20961307, DOI: 10.1111/j.1749-6632.2010.05712.x.Peer-Reviewed Original ResearchConceptsPolycystic kidney disease 1Yeast-bacterial shuttle vectorHuman disease genesFunction of genesLarge insert DNABacterial artificial chromosomeGene of interestTransgenic analysisGenomic fragmentArtificial chromosomesDNA insertsDisease genesBiological processesShuttle vectorHuman diseasesGenesGene modificationClaspettesPClasperMouse modelValuable resourceTransgenic mouse modelTransgenic miceCritical insightsImmune system
2007
Transgenic LacZ under control of Hec-6st regulatory sequences recapitulates endogenous gene expression on high endothelial venules
Liao S, Bentley K, Lebrun M, Lesslauer W, Ruddle FH, Ruddle NH. Transgenic LacZ under control of Hec-6st regulatory sequences recapitulates endogenous gene expression on high endothelial venules. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 4577-4582. PMID: 17360566, PMCID: PMC1838643, DOI: 10.1073/pnas.0700334104.Peer-Reviewed Original ResearchConceptsDNA fragmentsTertiary lymphoid organsExpression of reporterEndogenous gene expressionBAC DNA fragmentsTissue-specific expressionBeta-galactosidase reporter geneHomologous recombination techniquesLymphoid organsLymphoid tissueEffector genesBAC clonesEndogenous genesRegulatory sequencesNasal-associated lymphoid tissueReporter geneGene expressionLacZ constructLTbetaR-Ig treatmentExon IIHEV-like vesselsGenesHigh endothelial venulesMolecular natureRecombination techniques
1999
Murine neurofibroma reversion by antisense RNA for HTLV-I tax
Li C, Horowitz M, Ruddle N. Murine neurofibroma reversion by antisense RNA for HTLV-I tax. Science China Life Sciences 1999, 42: 8-16. PMID: 18726492, DOI: 10.1007/bf02881742.Peer-Reviewed Original ResearchAntisense RNATax proteinCellular genesEndogenous cellular genesReversed transcriptional orientationNeurofibroma cellsCell morphologyNeurofibroma cell linesTranscriptional orientationHTLV-I Tax proteinProto-oncogene expressionActin mRNAC-MycRetrovirus vectorGenesProteinRNACell linesTax geneMRNAM-CSFTax cDNAExpressionGrowth characteristicsPhenotype
1987
Lymphotoxin: Cloning, Regulation and Mechanism of Killing
Ruddle N, Li C, Tang W, Gray P, McGrath K. Lymphotoxin: Cloning, Regulation and Mechanism of Killing. Novartis Foundation Symposia 1987, 131: 64-87. PMID: 3502460, DOI: 10.1002/9780470513521.ch6.Peer-Reviewed Original ResearchConceptsCDNA libraryBamHI fragmentMurine genomic libraryFull-length cDNACOS-1 cellsCell linesT cell linesNorthern blot analysisGenomic libraryMechanism of killingCDNA probeDNA fragmentationChromosome 17T cell receptorRNABlot analysisCDNAGenesCell receptorCell clonesCellsTarget cellsFragmentsMRNA expressionCloning