2008
Expression and purification of functional recombinant human pigment epithelium-derived factor (PEDF) secreted by the yeast Pichia pastoris
Sánchez-Sánchez F, Aroca-Aguilar JD, Segura I, Ramírez-Castillejo C, Riese HH, Coca-Prados M, Escribano J. Expression and purification of functional recombinant human pigment epithelium-derived factor (PEDF) secreted by the yeast Pichia pastoris. Journal Of Biotechnology 2008, 134: 193-201. PMID: 18282627, DOI: 10.1016/j.jbiotec.2008.01.005.Peer-Reviewed Original ResearchConceptsPigment epithelium-derived factorEpithelium-derived factorRecombinant pigment epithelium-derived factorRecombinant human pigment epithelium-derived factorHuman pigment epithelium-derived factorCerebellar granule cell survivalPotential therapeutic agentGranule cell survivalFull-length PEDFStem cell self-renewal propertiesCell-based therapiesTherapeutic roleOcular diseasesTherapeutic agentsSelf-renewal propertiesEndothelial cell migrationLiquid chromatographyCell linesRPEDFCell survivalHigh-performance liquid chromatographyCell migrationYeast Pichia pastorisLow pressure liquid chromatographyPichia pastoris
1997
Molecular Characterization and Differential Gene Induction of the Neuroendocrine‐Specific Genes Neurotensin, Neurotensin Receptor, PC1, PC2, and 7B2 in the Human Ocular Ciliary Epithelium
Ortego J, Coca‐Prados M. Molecular Characterization and Differential Gene Induction of the Neuroendocrine‐Specific Genes Neurotensin, Neurotensin Receptor, PC1, PC2, and 7B2 in the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1997, 69: 1829-1839. PMID: 9349525, DOI: 10.1046/j.1471-4159.1997.69051829.x.Peer-Reviewed Original ResearchMeSH KeywordsAspartic Acid EndopeptidasesCell LineCiliary BodyColforsinDexamethasoneDNA PrimersEnzyme InductionEpithelial CellsFurinGene Expression RegulationHumansIsoproterenolModels, BiologicalNerve Growth FactorsNeurosecretory SystemsNeurotensinPolymerase Chain ReactionProprotein Convertase 2Proprotein ConvertasesReceptors, NeurotensinSubtilisinsTetradecanoylphorbol AcetateTimololTranscriptional ActivationUp-RegulationConceptsOcular ciliary epitheliumDifferential gene inductionImportant physiological functionsHuman ocular ciliary epitheliumHuman cell linesTransduction pathwaysGene inductionGene expressionCiliary epitheliumBeta2-adrenergic receptorMolecular characterizationPhysiological functionsCell extractsODM-2Human ciliary epitheliumNeuroepithelial cellsSame inducersPhorbol esterCell linesNeuroendocrine tissuesExpressionGrowth factorParacrine mechanismsPC2 expressionNerve growth factor
1996
Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor.
Ortego J, Escribano J, Becerra S, Coca-Prados M. Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor. Investigative Ophthalmology & Visual Science 1996, 37: 2759-67. PMID: 8977492.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAqueous HumorBlotting, NorthernCattleCell LineCells, CulturedChromatography, AffinityCiliary BodyDNA PrimersEye ProteinsFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoenzyme TechniquesNerve Growth FactorsPigment Epithelium of EyePolymerase Chain ReactionProtein BiosynthesisProteinsRabbitsSerpinsTranscription, GeneticConceptsPigment epithelium-derived factorEpithelium-derived factorPolymerase chain reactionCiliary epitheliumCiliary bodyOcular tissuesBlot analysisHuman ciliary epitheliumNeuronal survival activityHuman ocular ciliary epitheliumRetinal pigment epitheliumNorthern blot analysisCell linesBovine ocular tissuesPars plicata regionOcular ciliary bodyOcular ciliary epitheliumWestern blot analysisFactor antibodyPEDF expressionTrophic factorsPigment epitheliumPEDF proteinPEDF transcriptAqueous humor
1986
Growth of non-pigmented ciliary epithelial cells in serum-free hormone-supplemented media
Coca-Prados M, Chatt G. Growth of non-pigmented ciliary epithelial cells in serum-free hormone-supplemented media. Experimental Eye Research 1986, 43: 617-629. PMID: 3792464, DOI: 10.1016/s0014-4835(86)80028-8.Peer-Reviewed Original ResearchConceptsNPE cellsClathrin-coated vesiclesCiliary epithelial cellsNerve growth factorEpithelial cellsNon-pigmented ciliary epithelial cellsCellular polarityGrowth factorCellular junctionsPrimary culturesCoated pitsArtificial substratesTransport activityCulture mediumMicrofilament bundlesCell growthSerum-free hormone-supplemented mediumJunctional complexesIntermediate filamentsCell proliferationAdjacent cellsCellsSerum-free culture medium