2004
Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells
Al-Nakkash L, Iserovich P, Coca-Prados M, Yang H, Reinach P. Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells. The Journal Of Membrane Biology 2004, 201: 41-49. PMID: 15635811, DOI: 10.1007/s00232-004-0706-5.Peer-Reviewed Original ResearchConceptsCorneal epithelial cellsRabbit corneal epithelial cellsVolume-regulated anion channelsEpithelial cellsVoltage-dependent inhibitionHypotonic challengeVolume-activated chloride channelsRegulatory volume decrease (RVD) responseInactive phorbol ester analoguePresence of DIDSExtracellular calciumChloride currentsNiflumic acidRVD responseCell swellingDecrease responseNorthern blot analysisBlot analysisPhorbol dibutyratePhorbol ester analogsChloride channelsVACCNPPBMOsm mediumSV40-immortalized rabbit corneal epithelial cells
2001
Chloride dependent intracellular pH effects of external ATP in cultured human non-pigmented ciliary body epithelium
Cullinane A, Coca-Prados M, Harvey B. Chloride dependent intracellular pH effects of external ATP in cultured human non-pigmented ciliary body epithelium. Current Eye Research 2001, 23: 443-447. PMID: 12045894, DOI: 10.1076/ceyr.23.6.443.6967.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnti-Bacterial AgentsCalciumCells, CulturedChloride-Bicarbonate AntiportersChloridesCiliary BodyFluoresceinsFluorescent DyesHumansHydrogen-Ion ConcentrationMacrolidesMicroscopy, FluorescencePigment Epithelium of EyeProton-Translocating ATPasesReceptors, PurinergicSpectrometry, FluorescenceSuraminUp-RegulationConceptsNon-pigmented ciliary body epitheliumHNPE cellsCiliary body epitheliumAcetoxymethyl ester formBody epitheliumIntracellular calcium levelsCell-permeable calcium chelatorFluorescent probe BCECFReceptor inhibitorsIntracellular calciumPurinergic receptorsCalcium levelsDiisothiocyanatostilbene-2Extracellular adenosineSustained increaseMM suraminInhibitor amilorideExtracellular ATPCalcium chelatorEster formMicroM ATPATPase inhibitorEpitheliumInhibitorsIntracellular
2000
Tamoxifen and ATP synergistically activate Cl− release by cultured bovine pigmented ciliary epithelial cells
Mitchell C, Peterson‐Yantorno K, Coca‐Prados M, Civan M. Tamoxifen and ATP synergistically activate Cl− release by cultured bovine pigmented ciliary epithelial cells. The Journal Of Physiology 2000, 525: 183-193. PMID: 10811736, PMCID: PMC2269939, DOI: 10.1111/j.1469-7793.2000.00183.x.Peer-Reviewed Original Research
1996
Association of ClC-3 Channel with Cl− Transport by Human Nonpigmented Ciliary Epithelial Cells
Coca-Prados M, Sánchez-Torres J, Peterson-Yantorno K, Civan M. Association of ClC-3 Channel with Cl− Transport by Human Nonpigmented Ciliary Epithelial Cells. The Journal Of Membrane Biology 1996, 150: 197-208. PMID: 8661780, DOI: 10.1007/s002329900044.Peer-Reviewed Original Research
1992
Regulatory volume decrease by cultured non-pigmented ciliary epithelial cells
Civan M, Peterson-Yantorno K, Coca-Prados M, E.Yantorno R. Regulatory volume decrease by cultured non-pigmented ciliary epithelial cells. Experimental Eye Research 1992, 54: 181-191. PMID: 1559547, DOI: 10.1016/s0014-4835(05)80207-6.Peer-Reviewed Original ResearchWhole cell patch clamping of ciliary epithelial cells during anisosmotic swelling
Yantorno R, Carre D, Coca-Prados M, Krupin T, Civan M. Whole cell patch clamping of ciliary epithelial cells during anisosmotic swelling. American Journal Of Physiology 1992, 262: c501-c509. PMID: 1539636, DOI: 10.1152/ajpcell.1992.262.2.c501.Peer-Reviewed Original ResearchComparative Studies of Furosemide Effects on Membrane Potential and Intracellular Chloride Activity in Human and Rabbit Ciliary Epithelium
Chu T, Socci R, Coca-Prados M, Green K. Comparative Studies of Furosemide Effects on Membrane Potential and Intracellular Chloride Activity in Human and Rabbit Ciliary Epithelium. Ophthalmic Research 1992, 24: 83-91. PMID: 1608601, DOI: 10.1159/000267151.Peer-Reviewed Original Research
1989
Role of HCO3- in regulation of cytoplasmic pH in ciliary epithelial cells
Helbig H, Korbmacher C, Stumpff F, Coca-Prados M, Wiederholt M. Role of HCO3- in regulation of cytoplasmic pH in ciliary epithelial cells. American Journal Of Physiology 1989, 257: c696-c705. PMID: 2801920, DOI: 10.1152/ajpcell.1989.257.4.c696.Peer-Reviewed Original ResearchVolume regulation of cultured, transformed, non-pigmented epithelial cells from human ciliary body
Yantorno R, Coca-Prados M, Krupin T, Civan M. Volume regulation of cultured, transformed, non-pigmented epithelial cells from human ciliary body. Experimental Eye Research 1989, 49: 423-437. PMID: 2477268, DOI: 10.1016/0014-4835(89)90051-1.Peer-Reviewed Original ResearchElectrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium.
Helbig H, Korbmacher C, Wohlfarth J, Coca-Prados M, Wiederholt M. Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium. Investigative Ophthalmology & Visual Science 1989, 30: 882-9. PMID: 2470692.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidCalciumCell MembraneChloridesCiliary BodyClone CellsCyclamatesElectric ConductivityEpithelial CellsEpitheliumExtracellular SpaceHumansHydrogen-Ion ConcentrationOuabainPigmentationPotassiumSodiumConceptsIntracellular potentialsCiliary epitheliumCell clonesPresence of SITSPresence of ouabainElectrical membrane propertiesAddition of ouabainCiliary epithelial cellsMM EGTAEpithelial cellsTransient depolarizationMicroelectrode techniquesMembrane voltageCultured humanExtracellular ClCl- conductanceEpitheliumOuabainMembrane potentialIntracellular voltageFree solutionPotential response