2005
Retinoid processing proteins in the ocular ciliary epithelium.
Salvador-Silva M, Ghosh S, Bertazolli-Filho R, Boatright JH, Nickerson JM, Garwin GG, Saari JC, Coca-Prados M. Retinoid processing proteins in the ocular ciliary epithelium. Molecular Vision 2005, 11: 356-65. PMID: 15928609.Peer-Reviewed Original ResearchAcyltransferasesAlcohol OxidoreductasesAnimalsATP-Binding Cassette TransportersBlotting, WesternCarrier ProteinsCattleCells, CulturedChromatography, High Pressure LiquidCiliary BodyCis-trans-IsomerasesEye ProteinsFluorescent Antibody Technique, IndirectGene Expression RegulationHumansImmunohistochemistryPigment Epithelium of EyeProtein TransportRabbitsRetinoidsRetinol-Binding ProteinsRetinol-Binding Proteins, CellularReverse Transcriptase Polymerase Chain ReactionRNA, Messenger
2004
Inhibition of NHE-1 Na+/H+ exchanger by natriuretic peptides in ocular nonpigmented ciliary epithelium
Fidzinski P, Salvador-Silva M, Choritz L, Geibel J, Coca-Prados M. Inhibition of NHE-1 Na+/H+ exchanger by natriuretic peptides in ocular nonpigmented ciliary epithelium. American Journal Of Physiology - Cell Physiology 2004, 287: c655-c663. PMID: 15140751, DOI: 10.1152/ajpcell.00552.2003.Peer-Reviewed Original ResearchConceptsC-type natriuretic peptideAtrial natriuretic peptideBrain natriuretic peptideNatriuretic peptideIntraocular pressureCiliary epitheliumNHE activityNatriuretic peptides atrial natriuretic peptideInhibitory effectType B receptorsNHE-1 activityNonpigmented ciliary epitheliumCell layerGap junction blockersDose-dependent mannerInhibitors of NHEOcular ciliary epitheliumRate of intracellularHypotensive effectJunction blockersTrabecular meshworkMammalian eyeExchanger activityB receptorNHE-1
2003
Serum- and Glucocorticoid-Regulated Kinase Isoform-1 and Epithelial Sodium Channel Subunits in Human Ocular Ciliary Epithelium
Rauz S, Walker EA, Hughes SV, Coca-Prados M, Hewison M, Murray PI, Stewart PM. Serum- and Glucocorticoid-Regulated Kinase Isoform-1 and Epithelial Sodium Channel Subunits in Human Ocular Ciliary Epithelium. Investigative Ophthalmology & Visual Science 2003, 44: 1643-1651. PMID: 12657604, DOI: 10.1167/iovs.02-0514.Peer-Reviewed Original ResearchMeSH KeywordsAldosteroneBlotting, NorthernCell LineCiliary BodyDexamethasoneDose-Response Relationship, DrugDrug CombinationsEpithelial Sodium ChannelsGene Expression RegulationGlucocorticoidsHormone AntagonistsHumansImmediate-Early ProteinsIn Situ HybridizationIsoenzymesMifepristoneMineralocorticoid Receptor AntagonistsNuclear ProteinsPigment Epithelium of EyeProtein Serine-Threonine KinasesReceptors, GlucocorticoidReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodium ChannelsSpironolactoneConceptsExpression of SGK1Human ocular ciliary epitheliumODM-2 cellsOcular ciliary epitheliumSodium transportCiliary epitheliumENaC subunitsNPE cell linesEpithelial sodium channel (ENaC) subunitsAqueous humor productionSodium channel subunitsExpression of ENaCRadioligand-binding assaysDose-dependent inductionIsoform 1Epithelial sodium channelCorticosteroid regulationHuman ocularAldosteroneCiliary epithelial bilayerRU26752DexamethasoneRT-PCR analysisSodium channelsChannel subunitsCRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells
Kennedy BN, Li C, Ortego J, Coca-Prados M, Sarthy VP, Crabb JW. CRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells. Experimental Eye Research 2003, 76: 257-260. PMID: 12565814, DOI: 10.1016/s0014-4835(02)00308-1.Peer-Reviewed Original ResearchConceptsTranscriptional regulationBinding Protein FunctionsCell-specific expressionPromoter analysisProtein functionRepressor elementEnhancer elementsPromoter constructsReporter activityRetinal pigment epithelial cellsPigment epithelial cellsCiliary epitheliumEpithelial cellsVisual cycleRPE cellsBPRegulationRetinal pigment epitheliumCellsMüller cellsGenesMutationsRLBP1 geneEpitheliumWildtype
2002
Differential regulation of gene expression of neurotensin and prohormone convertases PC1 and PC2 in the bovine ocular ciliary epithelium: possible implications on neurotensin processing
Ortego J, Wollmann G, Coca-Prados M. Differential regulation of gene expression of neurotensin and prohormone convertases PC1 and PC2 in the bovine ocular ciliary epithelium: possible implications on neurotensin processing. Neuroscience Letters 2002, 333: 49-53. PMID: 12401558, DOI: 10.1016/s0304-3940(02)00028-9.Peer-Reviewed Original ResearchRenin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium
Cullinane A, Leung P, Ortego J, Coca-Prados M, Harvey B. Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium. British Journal Of Ophthalmology 2002, 86: 676. PMID: 12034692, PMCID: PMC1771149, DOI: 10.1136/bjo.86.6.676.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IICalciumCell SizeCells, CulturedCiliary BodyElectrophysiologyHumansLarge-Conductance Calcium-Activated Potassium ChannelsPatch-Clamp TechniquesPigment Epithelium of EyePotassium Channels, Calcium-ActivatedReceptors, AngiotensinRenin-Angiotensin SystemReverse Transcriptase Polymerase Chain ReactionSignal TransductionConceptsHNPE cellsAngiotensin IINon-pigmented epitheliumSecretory functionAddition of AIIRenin-angiotensin system componentsRenin-angiotensin system expressionIon channel activityPotassium ion channel activityActive BK channelsExpression of angiotensinogenAqueous humor dynamicsTreatment of glaucomaChannel activitySingle-channel patch-clamp techniquesPatch-clamp techniqueBK channel activationLarge-conductance potassium channelsHuman ciliary bodyPolymerase chain reaction analysisReceptor antagonistCurrent armamentariumCiliary bodyChain reaction analysisRAS expression
2001
Chloride dependent intracellular pH effects of external ATP in cultured human non-pigmented ciliary body epithelium
Cullinane A, Coca-Prados M, Harvey B. Chloride dependent intracellular pH effects of external ATP in cultured human non-pigmented ciliary body epithelium. Current Eye Research 2001, 23: 443-447. PMID: 12045894, DOI: 10.1076/ceyr.23.6.443.6967.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnti-Bacterial AgentsCalciumCells, CulturedChloride-Bicarbonate AntiportersChloridesCiliary BodyFluoresceinsFluorescent DyesHumansHydrogen-Ion ConcentrationMacrolidesMicroscopy, FluorescencePigment Epithelium of EyeProton-Translocating ATPasesReceptors, PurinergicSpectrometry, FluorescenceSuraminUp-RegulationConceptsNon-pigmented ciliary body epitheliumHNPE cellsCiliary body epitheliumAcetoxymethyl ester formBody epitheliumIntracellular calcium levelsCell-permeable calcium chelatorFluorescent probe BCECFReceptor inhibitorsIntracellular calciumPurinergic receptorsCalcium levelsDiisothiocyanatostilbene-2Extracellular adenosineSustained increaseMM suraminInhibitor amilorideExtracellular ATPCalcium chelatorEster formMicroM ATPATPase inhibitorEpitheliumInhibitorsIntracellularExtracellular ATP effects on calcium signaling in cultured human non-pigmented ciliary body epithelium
Cullinane A, Coca-Prados M, Harvey B. Extracellular ATP effects on calcium signaling in cultured human non-pigmented ciliary body epithelium. Current Eye Research 2001, 23: 448-454. PMID: 12045895, DOI: 10.1076/ceyr.23.6.448.6964.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateCalciumCalcium-Transporting ATPasesCells, CulturedCiliary BodyFluorescent DyesFura-2HumansMicroscopy, FluorescencePigment Epithelium of EyeReceptors, Purinergic P2Receptors, Purinergic P2Y2Signal TransductionSodium-Calcium ExchangerSpectrometry, FluorescenceThapsigarginConceptsNon-pigmented ciliary body epitheliumCiliary body epitheliumHNPE cellsIntracellular storesBody epitheliumFluorescent dye fura-2Extracellular ATP effectP2Y2 receptor activationDye fura-2Calcium signalsATPase pumpPresence of thapsigarginCapacitative calcium entrySodium-free solutionIntracellular calciumPurinergic receptorsFura-2Therapeutic targetCalcium entryPotency orderReceptor activationSustained increaseL-typeExtracellular ATPOscillatory increases
1997
Adenosine stimulates Cl− channels of nonpigmented ciliary epithelial cells
Carré D, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. Adenosine stimulates Cl− channels of nonpigmented ciliary epithelial cells. American Journal Of Physiology 1997, 273: c1354-c1361. PMID: 9357781, DOI: 10.1152/ajpcell.1997.273.4.c1354.Peer-Reviewed Original ResearchConceptsCiliary epithelial cellsNonpigmented ciliary epithelial cellsWhole-cell currentsEpithelial cellsAdenosine receptorsNPE cellsA2 adenosine receptorsMultiple purinergic receptorsCl- channel blockerHuman NPE cellsIntraocular pressureAdenosine actionPurinergic receptorsHCE cellsRabbit ciliary epitheliumCiliary epitheliumNiflumic acidBovine cellsReceptors
1996
Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor.
Ortego J, Escribano J, Becerra S, Coca-Prados M. Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor. Investigative Ophthalmology & Visual Science 1996, 37: 2759-67. PMID: 8977492.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAqueous HumorBlotting, NorthernCattleCell LineCells, CulturedChromatography, AffinityCiliary BodyDNA PrimersEye ProteinsFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoenzyme TechniquesNerve Growth FactorsPigment Epithelium of EyePolymerase Chain ReactionProtein BiosynthesisProteinsRabbitsSerpinsTranscription, GeneticConceptsPigment epithelium-derived factorEpithelium-derived factorPolymerase chain reactionCiliary epitheliumCiliary bodyOcular tissuesBlot analysisHuman ciliary epitheliumNeuronal survival activityHuman ocular ciliary epitheliumRetinal pigment epitheliumNorthern blot analysisCell linesBovine ocular tissuesPars plicata regionOcular ciliary bodyOcular ciliary epitheliumWestern blot analysisFactor antibodyPEDF expressionTrophic factorsPigment epitheliumPEDF proteinPEDF transcriptAqueous humor
1994
Isolation of cDNA clones encoding the 80-kd subunit protein of the human autoantigen Ku (p70/p80) by antisera raised against ciliary processes of human eye donors.
Hong T, Escribano J, Coca-Prados M. Isolation of cDNA clones encoding the 80-kd subunit protein of the human autoantigen Ku (p70/p80) by antisera raised against ciliary processes of human eye donors. Investigative Ophthalmology & Visual Science 1994, 35: 4023-30. PMID: 7960584.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, NuclearAutoantigensBlotting, WesternCell LineCiliary BodyClone CellsDNA HelicasesDNA, ComplementaryDNA-Binding ProteinsFluorescent Antibody TechniqueHumansImmunoglobulin GKu AutoantigenMaleMiceMice, Inbred BALB CMiddle AgedMolecular WeightNuclear ProteinsPigment Epithelium of EyeRNA, MessengerTissue DonorsConceptsHuman eye donorsPolymyositis overlap syndromeScleroderma-polymyositis overlap syndromeODM-2 cellsHuman ciliary processesCiliary processesOverlap syndromeEye donorsCiliary epitheliumSpecies-specific immune responseAnti-Ku antibodiesVariety of antigensCell linesEpithelium cell linePlaque-purified clonesCiliary epithelial cell lineOcular ciliary epitheliumWestern blot analysisSubunit proteinsEpithelial cell lineCiliary epithelial cellsImmune responseOcular tissuesPathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells.
Civan M, Coca-Prados M, Peterson-Yantorno K. Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells. Investigative Ophthalmology & Visual Science 1994, 35: 2876-86. PMID: 8188483.Peer-Reviewed Original ResearchConceptsRegulatory volume decreaseNonpigmented ciliary epithelial cellsProtein phosphatase inhibitor okadaic acidCiliary epithelial cellsPhosphatase inhibitor okadaic acidCultured nonpigmented ciliary epithelial cellsInhibitor okadaic acidEpithelial cellsNPE cellsHuman nonpigmented ciliary epithelial cellsCl- channelsCyclic AMPPKC inhibitor staurosporineEndogenous PKC activityOkadaic acidRegulatory responsesIntracellular cAMP concentrationCell typesPKC activatorCalcium-calmodulinPKC activityCell sortingElectronic cell sortingPathwayStaurosporine
1993
Differential Expression of the Cellular Retinaldehyde-binding Protein in Bovine Ciliary Epithelium
Martı̀n-Alonso J, Ghosh S, Hernando N, Crabb J, Coca-Prados M. Differential Expression of the Cellular Retinaldehyde-binding Protein in Bovine Ciliary Epithelium. Experimental Eye Research 1993, 56: 659-669. PMID: 8595808, DOI: 10.1006/exer.1993.1083.Peer-Reviewed Original ResearchCalcium-dependent regulation of cation transport in cultured human nonpigmented ciliary epithelial cells
Mito T, Delamere N, Coca-Prados M. Calcium-dependent regulation of cation transport in cultured human nonpigmented ciliary epithelial cells. American Journal Of Physiology 1993, 264: c519-c526. PMID: 8384781, DOI: 10.1152/ajpcell.1993.264.3.c519.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportBumetanideCalcimycinCalciumCarrier ProteinsCationsCell Line, TransformedCiliary BodyEgtazic AcidEpithelial CellsEpitheliumHumansOuabainPigment Epithelium of EyePotassium ChannelsQuinidineRubidiumSodium-Potassium-Chloride SymportersSodium-Potassium-Exchanging ATPaseTerpenesThapsigarginConceptsPotassium channelsIntracellular calcium bufferPotassium channel activityOuabain-sensitive potassium (86Rb) uptakeOuabain-sensitive componentCalcium-induced activationOuabain-insensitive componentNa-K-ATPaseCalcium-induced stimulationCiliary epithelial cellsNa-K-adenosinetriphosphataseMM quinidineThapsigargin stimulationIntracellular calciumPotassium lossCiliary epitheliumCalcium-dependent regulationEpithelial cellsAcetoxymethyl esterCalcium bufferCultured humanChannel activityCell linesCotransporterBumetanide
1992
Expression and cellular distribution of the al gap junction gene product in the ocular pigmented ciliary epithelium
Coca-Prados M, Ghosh S, Gilula N, Kumar N. Expression and cellular distribution of the al gap junction gene product in the ocular pigmented ciliary epithelium. Current Eye Research 1992, 11: 113-122. PMID: 1374005, DOI: 10.3109/02713689209000061.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsGene productsCiliary epithelial cellsApical plasma membrane domainPlasma membrane domainsEpithelial cellsAlpha 3Plasma membrane regionGap junction genesPE cellsNorthern blot analysisGap junction proteinImmunofluorescence localization studiesAlpha 1 transcriptsIndirect immunofluorescence localization studiesIntact tissueMembrane domainsAlpha 1 mRNABeta 1Predominant transcriptGap junction mRNAMembrane regionsJunction genesLocalization studiesCell types
1991
Detection and characterization of Ca2+-activated K+ channels in transformed cells of human non-pigmented ciliary epithelium
Barros F, Lòpez-Briones L, Coca-Prados M, Belmonte C. Detection and characterization of Ca2+-activated K+ channels in transformed cells of human non-pigmented ciliary epithelium. Current Eye Research 1991, 10: 731-738. PMID: 1914505, DOI: 10.3109/02713689109013867.Peer-Reviewed Original Research
1990
Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium.
Ghosh S, Freitag A, Martin-Vasallo P, Coca-Prados M. Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium. Journal Of Biological Chemistry 1990, 265: 2935-2940. PMID: 1689295, DOI: 10.1016/s0021-9258(19)39891-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernBlotting, WesternCattleCell MembraneCell Transformation, ViralCiliary BodyFlow CytometryFluorescent Antibody TechniqueGene ExpressionIsoenzymesMacromolecular SubstancesMicroscopy, ElectronPigment Epithelium of EyeRatsRNASimian virus 40Sodium-Potassium-Exchanging ATPaseConceptsAlpha-subunit isoformsAlpha isoformSubunit isoformsK-ATPaseBasolateral plasma membrane domainsK-ATPase alpha subunitPlasma membrane domainsImmunoreactive signalPE cellsDifferential gene expressionAlpha 3Alpha 3 polypeptidesNPE cellsCell linesAlpha 2Alpha 1 polypeptideAlpha 1Pattern of expressionAlpha 3 isoformAlpha 1 isoformMembrane domainsSpecific cDNA probesSimian virus 40Differential immunostaining patternAlpha 2 isoform
1989
Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells
Martin‐Vasallo P, Ghosh S, Coca‐Prados M. Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells. Journal Of Cellular Physiology 1989, 141: 243-252. PMID: 2553750, DOI: 10.1002/jcp.1041410203.Peer-Reviewed Original ResearchConceptsK-ATPase alpha subunit isoformsAlpha-subunit isoformsSubunit isoformsCultured cellsK-ATPase alpha subunitPE cellsExpression of NaBasolateral membrane domainsNorthern hybridization analysisNPE cell linesAlpha 2 mRNACell linesAlpha 3 isoformMembrane domainsAlpha 3 mRNAAlpha 1Alpha subunitCultured ciliary epithelial cellsWestern blot analysisHybridization analysisIsoformsCiliary epithelial cellsNPE layerBlot analysisK-ATPase
1988
Na+/H+ exchange regulates intracellular pH in a cell clone derived from bovine pigmented ciliary epithelium
Helbig H, Korbmacher C, Stumpff F, Coca‐Prados M, Wiederholt M. Na+/H+ exchange regulates intracellular pH in a cell clone derived from bovine pigmented ciliary epithelium. Journal Of Cellular Physiology 1988, 137: 384-389. PMID: 2848042, DOI: 10.1002/jcp.1041370225.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAnimalsCarrier ProteinsCattleClone CellsDose-Response Relationship, DrugHydrogen-Ion ConcentrationKineticsPigment Epithelium of EyeSodium-Hydrogen ExchangersConceptsPigmented ciliary epitheliumMM amilorideAcid loadCiliary epitheliumCell clonesPH-sensitive absorbanceBicarbonate-free mediumRegulation of intracellularCiliary bodyAmilorideExtracellular protonsBovine ciliary bodyEpitheliumConfluent monolayersIntracellularCellsCellular metabolismSlow acidificationAlkalinizationPlastic coverslips
1985
Separation of bovine pigmented ciliary epithelial cells by density gradient and further characterization in culture
Coca-Prados M, Kondo K. Separation of bovine pigmented ciliary epithelial cells by density gradient and further characterization in culture. Experimental Eye Research 1985, 40: 731-739. PMID: 3891394, DOI: 10.1016/0014-4835(85)90142-3.Peer-Reviewed Original Research