2001
Molecular Evidence That Human Ocular Ciliary Epithelium Expresses Components Involved in Phototransduction
Bertazolli-Filho R, Ghosh S, Huang W, Wollmann G, Coca-Prados M. Molecular Evidence That Human Ocular Ciliary Epithelium Expresses Components Involved in Phototransduction. Biochemical And Biophysical Research Communications 2001, 284: 317-325. PMID: 11394879, DOI: 10.1006/bbrc.2001.4970.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalArrestinBlotting, WesternCalcium-Binding ProteinsCattleCell LineCiliary BodyCyclic Nucleotide-Gated Cation ChannelsEpithelial CellsEye ProteinsFluorescent Antibody Technique, IndirectG-Protein-Coupled Receptor Kinase 1HippocalcinHumansImmunoblottingImmunohistochemistryIon ChannelsLight Signal TransductionLipoproteinsNerve Tissue ProteinsPhosphoric Diester HydrolasesProtein KinasesProtein SubunitsRecoverinRetinaReverse Transcriptase Polymerase Chain ReactionRhodopsinRNA, MessengerSequence Analysis, DNATransducinConceptsHuman ocular ciliary epitheliumCiliary epitheliumOcular ciliary epitheliumNPE cellsCiliary epithelial cell linePars plicata regionIntact ciliary epitheliumWestern blot analysisCultured NPE cellsEpithelial cell lineExpression of rhodopsinIndirect immunofluorescenceVisual arrestinHuman retinaMonoclonal antibodiesHuman ciliary epitheliumThree- to fourfoldEpitheliumRT-PCRPhotoreceptor cellsRhodopsin mRNABlot analysisCell linesRetinaRhodopsin kinase
1999
Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells
Sánchez-Torres J, Huang W, Civan M, Coca-Prados M. Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells. Current Eye Research 1999, 18: 408-416. PMID: 10435827, DOI: 10.1076/ceyr.18.6.408.5266.Peer-Reviewed Original ResearchConceptsOpen reading frameCiliary epithelial cellsPlasma membraneFusion proteinWestern blot analysisEpithelial cellsCellular distributionTag fusion proteinCandidate gene productsBlot analysisHuman nonpigmented ciliary epithelial cellsTotal cell extractsHypotonic treatmentHuman ciliary epithelial cellsMammalian cellsPolyclonal antibodiesReading frameGene productsNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsPerinuclear regionCellular localizationCell extractsCultured cellsProtein
1997
Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells
ORTEGO J, ESCRIBANO J, COCA-PRADOS M. Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells. Experimental Eye Research 1997, 65: 289-299. PMID: 9268597, DOI: 10.1006/exer.1997.0333.Peer-Reviewed Original ResearchMeSH KeywordsAgedAlpha 1-AntitrypsinAlpha-MacroglobulinsBlotting, NorthernBlotting, WesternCarcinogensCathepsin DCathepsin KCathepsinsCell LineCiliary BodyCysteine EndopeptidasesDNA, ComplementaryEndopeptidasesEpithelial CellsGene ExpressionGlycoproteinsHumansMiddle AgedPolymerase Chain ReactionPrecipitin TestsPregnancy ProteinsProtease InhibitorsSerine Proteinase InhibitorsTetradecanoylphorbol AcetateConceptsODM-2 cellsPP5/TFPICiliary epithelial cellsComplementary DNARegulation of transcriptionProteinase inhibitorsEpithelial cellsNon-protein kinase CCathepsin DProtein kinase C activationKinase C activationPattern of expressionCiliary bodyImmunoprecipitation experimentsGene expressionNorthern analysisCultured ciliary epithelial cellsKinase CWestern blot analysisHuman ciliary epitheliumAlpha-phorbol didecanoatePhorbol esterC activationBlot analysisHuman proteinases
1996
Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor.
Ortego J, Escribano J, Becerra S, Coca-Prados M. Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor. Investigative Ophthalmology & Visual Science 1996, 37: 2759-67. PMID: 8977492.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAqueous HumorBlotting, NorthernCattleCell LineCells, CulturedChromatography, AffinityCiliary BodyDNA PrimersEye ProteinsFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoenzyme TechniquesNerve Growth FactorsPigment Epithelium of EyePolymerase Chain ReactionProtein BiosynthesisProteinsRabbitsSerpinsTranscription, GeneticConceptsPigment epithelium-derived factorEpithelium-derived factorPolymerase chain reactionCiliary epitheliumCiliary bodyOcular tissuesBlot analysisHuman ciliary epitheliumNeuronal survival activityHuman ocular ciliary epitheliumRetinal pigment epitheliumNorthern blot analysisCell linesBovine ocular tissuesPars plicata regionOcular ciliary bodyOcular ciliary epitheliumWestern blot analysisFactor antibodyPEDF expressionTrophic factorsPigment epitheliumPEDF proteinPEDF transcriptAqueous humor
1994
Isolation of cDNA clones encoding the 80-kd subunit protein of the human autoantigen Ku (p70/p80) by antisera raised against ciliary processes of human eye donors.
Hong T, Escribano J, Coca-Prados M. Isolation of cDNA clones encoding the 80-kd subunit protein of the human autoantigen Ku (p70/p80) by antisera raised against ciliary processes of human eye donors. Investigative Ophthalmology & Visual Science 1994, 35: 4023-30. PMID: 7960584.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, NuclearAutoantigensBlotting, WesternCell LineCiliary BodyClone CellsDNA HelicasesDNA, ComplementaryDNA-Binding ProteinsFluorescent Antibody TechniqueHumansImmunoglobulin GKu AutoantigenMaleMiceMice, Inbred BALB CMiddle AgedMolecular WeightNuclear ProteinsPigment Epithelium of EyeRNA, MessengerTissue DonorsConceptsHuman eye donorsPolymyositis overlap syndromeScleroderma-polymyositis overlap syndromeODM-2 cellsHuman ciliary processesCiliary processesOverlap syndromeEye donorsCiliary epitheliumSpecies-specific immune responseAnti-Ku antibodiesVariety of antigensCell linesEpithelium cell linePlaque-purified clonesCiliary epithelial cell lineOcular ciliary epitheliumWestern blot analysisSubunit proteinsEpithelial cell lineCiliary epithelial cellsImmune responseOcular tissues
1989
Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells
Martin‐Vasallo P, Ghosh S, Coca‐Prados M. Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells. Journal Of Cellular Physiology 1989, 141: 243-252. PMID: 2553750, DOI: 10.1002/jcp.1041410203.Peer-Reviewed Original ResearchConceptsK-ATPase alpha subunit isoformsAlpha-subunit isoformsSubunit isoformsCultured cellsK-ATPase alpha subunitPE cellsExpression of NaBasolateral membrane domainsNorthern hybridization analysisNPE cell linesAlpha 2 mRNACell linesAlpha 3 isoformMembrane domainsAlpha 3 mRNAAlpha 1Alpha subunitCultured ciliary epithelial cellsWestern blot analysisHybridization analysisIsoformsCiliary epithelial cellsNPE layerBlot analysisK-ATPase