1997
Molecular Characterization and Differential Gene Induction of the Neuroendocrine‐Specific Genes Neurotensin, Neurotensin Receptor, PC1, PC2, and 7B2 in the Human Ocular Ciliary Epithelium
Ortego J, Coca‐Prados M. Molecular Characterization and Differential Gene Induction of the Neuroendocrine‐Specific Genes Neurotensin, Neurotensin Receptor, PC1, PC2, and 7B2 in the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1997, 69: 1829-1839. PMID: 9349525, DOI: 10.1046/j.1471-4159.1997.69051829.x.Peer-Reviewed Original ResearchMeSH KeywordsAspartic Acid EndopeptidasesCell LineCiliary BodyColforsinDexamethasoneDNA PrimersEnzyme InductionEpithelial CellsFurinGene Expression RegulationHumansIsoproterenolModels, BiologicalNerve Growth FactorsNeurosecretory SystemsNeurotensinPolymerase Chain ReactionProprotein Convertase 2Proprotein ConvertasesReceptors, NeurotensinSubtilisinsTetradecanoylphorbol AcetateTimololTranscriptional ActivationUp-RegulationConceptsOcular ciliary epitheliumDifferential gene inductionImportant physiological functionsHuman ocular ciliary epitheliumHuman cell linesTransduction pathwaysGene inductionGene expressionCiliary epitheliumBeta2-adrenergic receptorMolecular characterizationPhysiological functionsCell extractsODM-2Human ciliary epitheliumNeuroepithelial cellsSame inducersPhorbol esterCell linesNeuroendocrine tissuesExpressionGrowth factorParacrine mechanismsPC2 expressionNerve growth factor
1995
Immortalization of cat iris sphincter smooth muscle cells by SV40 virus: Growth, morphological, biochemical and pharmacological characteristics
Ocklind A, Yousufzai S, Ghosh S, Coca-Prados M, St Jernschantz J, Abdel-Latif A. Immortalization of cat iris sphincter smooth muscle cells by SV40 virus: Growth, morphological, biochemical and pharmacological characteristics. Experimental Eye Research 1995, 61: 535-545. PMID: 8654496, DOI: 10.1016/s0014-4835(05)80047-8.Peer-Reviewed Original ResearchConceptsSignal transduction pathwaysCat iris sphincter smooth muscle cellsSmooth muscle alpha-actinMuscle alpha-actinImmortalized cellsTransduction pathwaysSmooth muscle cellsMuscle cellsAlpha-actinImportant model systemSV40 virusLarge T antigenImmortalized cell culturesMlc proteinNormal muscle cellsTransformation phenotypeSuccessful immortalizationLow-serum mediumCellular DNAContact inhibitionPopulation doublingsSV-CISM-2 cellsT antigenViral transformationArachidonic acid