2005
Selective Upregulation of the A3 Adenosine Receptor in Eyes with Pseudoexfoliation Syndrome and Glaucoma
Schlötzer-Schrehardt U, Zenkel M, Decking U, Haubs D, Kruse FE, Jünemann A, Coca-Prados M, Naumann GO. Selective Upregulation of the A3 Adenosine Receptor in Eyes with Pseudoexfoliation Syndrome and Glaucoma. Investigative Ophthalmology & Visual Science 2005, 46: 2023-2034. PMID: 15914619, DOI: 10.1167/iovs.04-0915.Peer-Reviewed Original ResearchMeSH KeywordsAdenosineAgedAged, 80 and overAnterior Eye SegmentAqueous HumorBlotting, NorthernCell Culture TechniquesChromatography, High Pressure LiquidExfoliation SyndromeGlaucoma, Angle-ClosureGlaucoma, Open-AngleHumansHypoxanthineImmunohistochemistryIn Situ HybridizationInosineReceptor, Adenosine A3Reverse Transcriptase Polymerase Chain ReactionRNA, MessengerUp-RegulationXanthineConceptsControl eyesLevels of adenosineA3 adenosine receptorAdenosine receptorsPEX eyesPseudoexfoliation syndromeIntraocular pressureAqueous humor secretionCiliary epitheliumEpithelial cellsNonpigmented epithelial cellsOxidative stressGlaucomatous control eyesAngle-closure glaucomaNormal control eyesHypoxia-induced upregulationAdenosine receptor expressionAqueous humor samplesAnterior segment tissuesAqueous humor dynamicsNonpigmented ciliary epitheliumA3 receptor mRNAEffects of hypoxiaAdenosine receptor subtypesNonpigmented ciliary epithelial cellsFOXC1 Transcriptional Regulatory Activity Is Impaired by PBX1 in a Filamin A-Mediated Manner
Berry FB, O'Neill MA, Coca-Prados M, Walter MA. FOXC1 Transcriptional Regulatory Activity Is Impaired by PBX1 in a Filamin A-Mediated Manner. Molecular And Cellular Biology 2005, 25: 1415-1424. PMID: 15684392, PMCID: PMC548007, DOI: 10.1128/mcb.25.4.1415-1424.2005.Peer-Reviewed Original ResearchMeSH KeywordsCell FractionationCell NucleusChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneContractile ProteinsDNA-Binding ProteinsEye AbnormalitiesFilaminsForkhead Transcription FactorsHeLa CellsHeterochromatinHumansMicrofilament ProteinsMutationProtein BindingProto-Oncogene ProteinsTranscription FactorsTumor Cells, CulturedConceptsFilamin AHuman nonpigmented ciliary epithelial cellsFunction mutationsNonpigmented ciliary epithelial cellsAxenfeld-Rieger syndromeAutosomal dominant disorderCiliary epithelial cellsMelanoma cellsSkeletal disordersElevated levelsDominant disorderEpithelial cellsImportant transcription factorSkeletal phenotypeProtein filamin AFOXC1Inhibitory activityDisorders
2004
Hyposmotic activation of ICl,swell in rabbit nonpigmented ciliary epithelial cells involves increased ClC-3 trafficking to the plasma membrane
Vessey JP, Shi C, Jollimore CA, Stevens KT, Coca-Prados M, Barnes S, Kelly ME. Hyposmotic activation of ICl,swell in rabbit nonpigmented ciliary epithelial cells involves increased ClC-3 trafficking to the plasma membrane. Biochemistry And Cell Biology 2004, 82: 708-718. PMID: 15674438, DOI: 10.1139/o04-107.Peer-Reviewed Original ResearchConceptsPlasma membraneHyposmotic stimulationFluorescent membrane dye FM1-43Membrane dye FM1-43Trafficking of channelsClC-3 channelsCiliary epithelial cellsPhosphoinositide-3 kinase inhibitor wortmanninRate of exocytosisClC-3 Cl(-) channelsEpithelial cellsVesicular traffickingDye FM1-43Membrane dynamicsNonpigmented ciliary epithelial cellsInhibitor wortmanninClC-3 antisenseImmunofluorescence microscopyTraffickingClC-3Regulatory volume decreaseFM1-43Cl- channelsMembraneCell swelling
2003
A3 adenosine and CB1 receptors activate a PKC‐sensitive Cl− current in human nonpigmented ciliary epithelial cells via a Gβγ‐coupled MAPK signaling pathway
Shi C, Szczesniak A, Mao L, Jollimore C, Coca‐Prados M, Hung O, Kelly ME. A3 adenosine and CB1 receptors activate a PKC‐sensitive Cl− current in human nonpigmented ciliary epithelial cells via a Gβγ‐coupled MAPK signaling pathway. British Journal Of Pharmacology 2003, 139: 475-486. PMID: 12788807, PMCID: PMC1573867, DOI: 10.1038/sj.bjp.0705266.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TransformedChloride ChannelsCiliaDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsEpithelial CellsGTP-Binding Protein beta SubunitsGTP-Binding Protein gamma SubunitsHumansMAP Kinase Signaling SystemProtein Kinase CReceptor, Adenosine A3Receptor, Cannabinoid, CB1ConceptsCB1 receptorsCl- currentSR 141716Receptor agonistNPCE cellsA3 adenosineWhole-cell patch-clamp recordingsCB1 receptor inverse agonistBasal Cl(-) currentCB1 receptor agonistA3 receptor agonistPatch-clamp recordingsHuman CB1 receptorReceptor-transfected cellsReceptor inverse agonistHuman nonpigmented ciliary epithelial cellsSignaling pathwaysAdenosine receptor antagonistNonpigmented ciliary epithelial cellsCiliary epithelial cell linePI3K inhibitorsMitogen-activated protein kinase kinase inhibitorBeta-adrenergic receptor kinaseProtein kinase kinase inhibitorEpithelial cell line
2002
Protein tyrosine kinase and protein phosphatase signaling pathways regulate volume-sensitive chloride currents in a nonpigmented ciliary epithelial cell line.
Shi C, Barnes S, Coca-Prados M, Kelly ME. Protein tyrosine kinase and protein phosphatase signaling pathways regulate volume-sensitive chloride currents in a nonpigmented ciliary epithelial cell line. Investigative Ophthalmology & Visual Science 2002, 43: 1525-32. PMID: 11980870.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TransformedChloride ChannelsCiliary BodyEnzyme ActivationEnzyme InhibitorsEpithelial CellsPatch-Clamp TechniquesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphoprotein PhosphatasesPhosphorylationProtein-Tyrosine KinasesRabbitsSignal TransductionConceptsProtein tyrosine kinasesProtein tyrosine kinase inhibitorTyrosine kinaseOkadaic acidPP-1Ciliary epithelial cellsHyposmotic stimulationC-Src tyrosine kinasePI3KProtein phosphatase inhibitorPKC inhibitorEpithelial cellsSrc tyrosine kinaseCiliary epithelial cell linePhosphatidylinositol-3 kinase inhibitorKinase inhibitorsProtein phosphatasePhosphatase inhibitorCl- currentEpithelial cell lineNonpigmented ciliary epithelial cellsCell swellingKinasePKC activatorTyrosine kinase inhibitors
2001
PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells
Fleischhauer J, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2001, 281: c1614-c1623. PMID: 11600425, DOI: 10.1152/ajpcell.2001.281.5.c1614.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsCiliary epithelial cellsCl- channelsEpithelial cellsPE cellsCl- channel activityAction of nucleotidesProtein kinaseNonpigmented ciliary epithelial cellsSecond messengerCell sortingChannel activityATP activationElectronic cell sortingATPSequential cascadeChannel activationNucleotidesP2Y receptorsPatch clampingATP assayATP releaseCellsMicroM ATPCAMP
2000
Similarity of A3-adenosine and swelling-activated Cl− channels in nonpigmented ciliary epithelial cells
Carré D, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. Similarity of A3-adenosine and swelling-activated Cl− channels in nonpigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2000, 279: c440-c451. PMID: 10913011, DOI: 10.1152/ajpcell.2000.279.2.c440.Peer-Reviewed Original Research
1999
Functional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells
Ortego J, Coca-Prados M. Functional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells. Biochemical And Biophysical Research Communications 1999, 258: 21-28. PMID: 10222228, DOI: 10.1006/bbrc.1999.0573.Peer-Reviewed Original ResearchConceptsNatriuretic peptide systemNPR-B receptorNPR-C receptorCiliary epithelial cellsReceptor mRNACiliary epitheliumEpithelial cellsNPE cellsCGMP responseAutocrine/paracrine modulatorPeptide systemHuman ocular ciliary epitheliumNonpigmented ciliary epithelial cellsOcular ciliary epitheliumCultured NPE cellsLong-term downregulationC-ANP4Paracrine modulatorEye pressureBNPActivation of PKCBasal levelsInhibitory effectRT-PCRReceptorsA3 adenosine receptors regulate Cl−channels of nonpigmented ciliary epithelial cells
Mitchell C, Peterson-Yantorno K, Carré D, McGlinn A, Coca-Prados M, Stone R, Civan M. A3 adenosine receptors regulate Cl−channels of nonpigmented ciliary epithelial cells. American Journal Of Physiology 1999, 276: c659-c666. PMID: 10069993, DOI: 10.1152/ajpcell.1999.276.3.c659.Peer-Reviewed Original ResearchConceptsAgonist N6HCE cellsIB-MECAA3 receptorsAdenosine receptorsCiliary processesA2A agonist CGS 21680Rabbit ciliary processesCiliary epitheliumA1-selective agonist N6Specific adenosine receptorsAgonist CGS 21680NPE cellsCl- channelsNonpigmented ciliary epithelial cellsA3 adenosine receptorHuman NPE cellsCGS 21680Ciliary epithelial cellsReceptor subtypesIntracellular Ca2MicroM adenosineRabbit ciliary epitheliumRT-PCREpithelial cellsEffects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells
Sánchez-Torres J, Huang W, Civan M, Coca-Prados M. Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells. Current Eye Research 1999, 18: 408-416. PMID: 10435827, DOI: 10.1076/ceyr.18.6.408.5266.Peer-Reviewed Original ResearchConceptsOpen reading frameCiliary epithelial cellsPlasma membraneFusion proteinWestern blot analysisEpithelial cellsCellular distributionTag fusion proteinCandidate gene productsBlot analysisHuman nonpigmented ciliary epithelial cellsTotal cell extractsHypotonic treatmentHuman ciliary epithelial cellsMammalian cellsPolyclonal antibodiesReading frameGene productsNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsPerinuclear regionCellular localizationCell extractsCultured cellsProtein
1997
Adenosine stimulates Cl− channels of nonpigmented ciliary epithelial cells
Carré D, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. Adenosine stimulates Cl− channels of nonpigmented ciliary epithelial cells. American Journal Of Physiology 1997, 273: c1354-c1361. PMID: 9357781, DOI: 10.1152/ajpcell.1997.273.4.c1354.Peer-Reviewed Original ResearchConceptsCiliary epithelial cellsNonpigmented ciliary epithelial cellsWhole-cell currentsEpithelial cellsAdenosine receptorsNPE cellsA2 adenosine receptorsMultiple purinergic receptorsCl- channel blockerHuman NPE cellsIntraocular pressureAdenosine actionPurinergic receptorsHCE cellsRabbit ciliary epitheliumCiliary epitheliumNiflumic acidBovine cellsReceptors
1996
Identification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium
Ortego J, Escribano J, Crabb J, Coca‐Prados M. Identification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1996, 66: 787-796. PMID: 8592153, DOI: 10.1046/j.1471-4159.1996.66020787.x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnimalsAqueous HumorBase SequenceCarboxypeptidase HCarboxypeptidasesCell LineChild, PreschoolChromograninsCiliary BodyEpithelial CellsEpitheliumFluorescent Antibody Technique, IndirectHumansMixed Function OxygenasesMolecular Sequence DataMultienzyme ComplexesNeurosecretory SystemsProteinsRatsConceptsNeuropeptide processing enzymesCarboxypeptidase ECiliary epithelial cellsOcular ciliary epitheliumSecretogranin IICell-cell communicationEpithelial cellsHuman ocular ciliary epitheliumCDNA clonesCell signalingNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsProcessing enzymesMolecular markersPeptidylglycine alphaPhorbol esterSpecialized groupSpecific probesMammalian eyeNeuroendocrine tissuesEnzymeCiliary epitheliumKey functionsCellsPolymerase chain reaction
1995
Cell-specific expression of the human Na+,K(+)-ATPase beta 2 subunit isoform in the nonpigmented ciliary epithelium.
Coca-Prados M, Fernández-Cabezudo M, Sánchez-Torres J, Crabb J, Ghosh S. Cell-specific expression of the human Na+,K(+)-ATPase beta 2 subunit isoform in the nonpigmented ciliary epithelium. Investigative Ophthalmology & Visual Science 1995, 36: 2717-28. PMID: 7499094.Peer-Reviewed Original ResearchConceptsBeta 2 isoformNonpigmented ciliary epithelial cellsOcular tissuesCiliary epitheliumBeta 1NPE cell linesNonpigmented ciliary epitheliumNPE cellsHuman ocular tissuesSubunit isoformsBlot analysisATPase subunit isoformsSpecific anti-peptide antibodiesRestricted cellular distributionPolymerase chain reactionCiliary epithelial cellsBeta-subunit isoformsAlpha-subunit isoformsAnti-peptide antibodiesODM-2Polymerase chain reaction amplificationChain reaction amplificationHuman ciliary epitheliumCell-specific distributionPattern of expression
1994
Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells.
Civan M, Coca-Prados M, Peterson-Yantorno K. Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells. Investigative Ophthalmology & Visual Science 1994, 35: 2876-86. PMID: 8188483.Peer-Reviewed Original ResearchConceptsRegulatory volume decreaseNonpigmented ciliary epithelial cellsProtein phosphatase inhibitor okadaic acidCiliary epithelial cellsPhosphatase inhibitor okadaic acidCultured nonpigmented ciliary epithelial cellsInhibitor okadaic acidEpithelial cellsNPE cellsHuman nonpigmented ciliary epithelial cellsCl- channelsCyclic AMPPKC inhibitor staurosporineEndogenous PKC activityOkadaic acidRegulatory responsesIntracellular cAMP concentrationCell typesPKC activatorCalcium-calmodulinPKC activityCell sortingElectronic cell sortingPathwayStaurosporine