2017
Quantitative study of zinc and metallothioneins in the human retina and RPE cells by mass spectrometry-based methodologies
Rodríguez-Menéndez S, Fernández B, García M, Álvarez L, Fernández M, Sanz-Medel A, Coca-Prados M, Pereiro R, González-Iglesias H. Quantitative study of zinc and metallothioneins in the human retina and RPE cells by mass spectrometry-based methodologies. Talanta 2017, 178: 222-230. PMID: 29136815, DOI: 10.1016/j.talanta.2017.09.024.Peer-Reviewed Original Research
2014
Quantitative selenium speciation by HPLC-ICP-MS(IDA) and simultaneous activity measurements in human vitreous humor
González de Vega R, Fernández-Sánchez ML, González Iglesias H, Coca Prados M, Sanz-Medel A. Quantitative selenium speciation by HPLC-ICP-MS(IDA) and simultaneous activity measurements in human vitreous humor. Analytical And Bioanalytical Chemistry 2014, 407: 2405-2413. PMID: 25344931, DOI: 10.1007/s00216-014-8241-6.Peer-Reviewed Original ResearchConceptsSize exclusion chromatographyMain selenium speciesQuantitative selenium speciationPlasma mass spectrometryQuantitative speciationSEC-ICPSelenium speciationSelenium speciesProtein complexesExclusion chromatographyExpected molecular weightSimultaneous activity measurementsMass spectrometryHPLC-ICPGPx proteinAntioxidant enzyme activitiesVitreous humor samplesICP-MSMolecular weightTotal Se concentrationEnzymatic activityPpb SeHuman vitreous humorEnzyme activityComplexes