1993
Calcium-dependent regulation of cation transport in cultured human nonpigmented ciliary epithelial cells
Mito T, Delamere N, Coca-Prados M. Calcium-dependent regulation of cation transport in cultured human nonpigmented ciliary epithelial cells. American Journal Of Physiology 1993, 264: c519-c526. PMID: 8384781, DOI: 10.1152/ajpcell.1993.264.3.c519.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportBumetanideCalcimycinCalciumCarrier ProteinsCationsCell Line, TransformedCiliary BodyEgtazic AcidEpithelial CellsEpitheliumHumansOuabainPigment Epithelium of EyePotassium ChannelsQuinidineRubidiumSodium-Potassium-Chloride SymportersSodium-Potassium-Exchanging ATPaseTerpenesThapsigarginConceptsPotassium channelsIntracellular calcium bufferPotassium channel activityOuabain-sensitive potassium (86Rb) uptakeOuabain-sensitive componentCalcium-induced activationOuabain-insensitive componentNa-K-ATPaseCalcium-induced stimulationCiliary epithelial cellsNa-K-adenosinetriphosphataseMM quinidineThapsigargin stimulationIntracellular calciumPotassium lossCiliary epitheliumCalcium-dependent regulationEpithelial cellsAcetoxymethyl esterCalcium bufferCultured humanChannel activityCell linesCotransporterBumetanide
1989
Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium.
Helbig H, Korbmacher C, Wohlfarth J, Coca-Prados M, Wiederholt M. Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium. Investigative Ophthalmology & Visual Science 1989, 30: 882-9. PMID: 2470692.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidCalciumCell MembraneChloridesCiliary BodyClone CellsCyclamatesElectric ConductivityEpithelial CellsEpitheliumExtracellular SpaceHumansHydrogen-Ion ConcentrationOuabainPigmentationPotassiumSodiumConceptsIntracellular potentialsCiliary epitheliumCell clonesPresence of SITSPresence of ouabainElectrical membrane propertiesAddition of ouabainCiliary epithelial cellsMM EGTAEpithelial cellsTransient depolarizationMicroelectrode techniquesMembrane voltageCultured humanExtracellular ClCl- conductanceEpitheliumOuabainMembrane potentialIntracellular voltageFree solutionPotential responseEffect of acetylcholine on membrane potential of cultured human nonpigmented ciliary epithelial cells.
Helbig H, Korbmacher C, Wohlfarth J, Coroneo M, Lindschau C, Quass P, Haller H, Coca-Prados M, Wiederholt M. Effect of acetylcholine on membrane potential of cultured human nonpigmented ciliary epithelial cells. Investigative Ophthalmology & Visual Science 1989, 30: 890-6. PMID: 2722445.Peer-Reviewed Original ResearchConceptsAcetylcholine-induced responsesCiliary epithelial cellsCytoplasmic Ca2Epithelial cellsEffects of acetylcholineAddition of acetylcholineMembrane potentialMobilization of Ca2M atropineRole of Ca2M acetylcholineMM quinidineChannel blockersFura-2Sustained depolarizationAcetylcholineIntracellular storesInitial hyperpolarizationTransient hyperpolarizationCell membrane potentialMicroelectrode techniquesCultured humanHyperpolarizationFree mediumOrigin-defective mutant