1999
Differential gene expression in the human ciliary epithelium
Coca-Prados M, Escribano J, Ortego J. Differential gene expression in the human ciliary epithelium. Progress In Retinal And Eye Research 1999, 18: 403-429. PMID: 10192520, DOI: 10.1016/s1350-9462(98)00026-3.Peer-Reviewed Original ResearchConceptsDifferential gene expressionCell-specific expressionDiversity of functionsExpression of genesOcular ciliary bodySubtractive libraryNeuropeptide processing enzymesMolecular basisGene expressionCultured ciliary epithelial cellsCell differentiationCiliary epitheliumHuman ciliary epitheliumAntioxidative enzymesGenesCiliary epithelial cellsRegulatory peptidesLight-dark cycleEpithelial cellsMuscle cellsCiliary muscle cellsAutocrine mechanismExpressionPlasma proteinsParacrine mechanismsEffects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells
Sánchez-Torres J, Huang W, Civan M, Coca-Prados M. Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells. Current Eye Research 1999, 18: 408-416. PMID: 10435827, DOI: 10.1076/ceyr.18.6.408.5266.Peer-Reviewed Original ResearchConceptsOpen reading frameCiliary epithelial cellsPlasma membraneFusion proteinWestern blot analysisEpithelial cellsCellular distributionTag fusion proteinCandidate gene productsBlot analysisHuman nonpigmented ciliary epithelial cellsTotal cell extractsHypotonic treatmentHuman ciliary epithelial cellsMammalian cellsPolyclonal antibodiesReading frameGene productsNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsPerinuclear regionCellular localizationCell extractsCultured cellsProtein
1997
Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells
ORTEGO J, ESCRIBANO J, COCA-PRADOS M. Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells. Experimental Eye Research 1997, 65: 289-299. PMID: 9268597, DOI: 10.1006/exer.1997.0333.Peer-Reviewed Original ResearchMeSH KeywordsAgedAlpha 1-AntitrypsinAlpha-MacroglobulinsBlotting, NorthernBlotting, WesternCarcinogensCathepsin DCathepsin KCathepsinsCell LineCiliary BodyCysteine EndopeptidasesDNA, ComplementaryEndopeptidasesEpithelial CellsGene ExpressionGlycoproteinsHumansMiddle AgedPolymerase Chain ReactionPrecipitin TestsPregnancy ProteinsProtease InhibitorsSerine Proteinase InhibitorsTetradecanoylphorbol AcetateConceptsODM-2 cellsPP5/TFPICiliary epithelial cellsComplementary DNARegulation of transcriptionProteinase inhibitorsEpithelial cellsNon-protein kinase CCathepsin DProtein kinase C activationKinase C activationPattern of expressionCiliary bodyImmunoprecipitation experimentsGene expressionNorthern analysisCultured ciliary epithelial cellsKinase CWestern blot analysisHuman ciliary epitheliumAlpha-phorbol didecanoatePhorbol esterC activationBlot analysisHuman proteinases
1996
Identification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium
Ortego J, Escribano J, Crabb J, Coca‐Prados M. Identification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1996, 66: 787-796. PMID: 8592153, DOI: 10.1046/j.1471-4159.1996.66020787.x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnimalsAqueous HumorBase SequenceCarboxypeptidase HCarboxypeptidasesCell LineChild, PreschoolChromograninsCiliary BodyEpithelial CellsEpitheliumFluorescent Antibody Technique, IndirectHumansMixed Function OxygenasesMolecular Sequence DataMultienzyme ComplexesNeurosecretory SystemsProteinsRatsConceptsNeuropeptide processing enzymesCarboxypeptidase ECiliary epithelial cellsOcular ciliary epitheliumSecretogranin IICell-cell communicationEpithelial cellsHuman ocular ciliary epitheliumCDNA clonesCell signalingNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsProcessing enzymesMolecular markersPeptidylglycine alphaPhorbol esterSpecialized groupSpecific probesMammalian eyeNeuroendocrine tissuesEnzymeCiliary epitheliumKey functionsCellsPolymerase chain reaction
1989
Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells
Martin‐Vasallo P, Ghosh S, Coca‐Prados M. Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells. Journal Of Cellular Physiology 1989, 141: 243-252. PMID: 2553750, DOI: 10.1002/jcp.1041410203.Peer-Reviewed Original ResearchConceptsK-ATPase alpha subunit isoformsAlpha-subunit isoformsSubunit isoformsCultured cellsK-ATPase alpha subunitPE cellsExpression of NaBasolateral membrane domainsNorthern hybridization analysisNPE cell linesAlpha 2 mRNACell linesAlpha 3 isoformMembrane domainsAlpha 3 mRNAAlpha 1Alpha subunitCultured ciliary epithelial cellsWestern blot analysisHybridization analysisIsoformsCiliary epithelial cellsNPE layerBlot analysisK-ATPase
1987
Evidence that the α and α(+) isoforms of the catalytic subunit of (Na+,K+)-ATPase reside in distinct ciliary epithelial cells of the mammalian eye
Coca-Prados M, López-Briones L. Evidence that the α and α(+) isoforms of the catalytic subunit of (Na+,K+)-ATPase reside in distinct ciliary epithelial cells of the mammalian eye. Biochemical And Biophysical Research Communications 1987, 145: 460-466. PMID: 3036129, DOI: 10.1016/0006-291x(87)91343-x.Peer-Reviewed Original ResearchConceptsCatalytic subunitCiliary epithelial cellsEpithelial cellsNPE cellsCultured ciliary epithelial cellsBasolateral surfaceImmunoblot analysisCiliary processesIntact ciliary processesMammalian eyeATPasePE cellsSubunitsPolyclonal antibodiesCanine kidneyCellsAlpha formAlphaIndirect immunofluorescenceIsoformsProteinBovine eyes
1985
Regulation of protein phosphorylation of the intermediate-sized filament vimentin in the ciliary epithelium of the mammalian eye.
Coca-Prados M. Regulation of protein phosphorylation of the intermediate-sized filament vimentin in the ciliary epithelium of the mammalian eye. Journal Of Biological Chemistry 1985, 260: 10332-10338. PMID: 4019517, DOI: 10.1016/s0021-9258(17)39252-9.Peer-Reviewed Original ResearchConceptsBeta-adrenergic agonistsMammalian eyeAlpha-adrenergic agonist phenylephrineBeta-adrenergic antagonist timololCiliary epitheliumCultured ciliary epithelial cellsCyclic AMPCellular cyclic AMPEpithelial-derived cellsAgonist phenylephrineCiliary epithelial cellsProstaglandin E1Catecholamine hormonesMicroM forskolinSerum-free mediumCiliary processesMonoclonal antibodiesRabbit ciliary processesVimentinAdenylate cyclaseEpithelial cellsPhosphorylation of vimentinIndirect immunofluorescence microscopyIntermediate-sized filamentsAgonists