2017
Quantitative distribution of Zn, Fe and Cu in the human lens and study of the Zn–metallothionein redox system in cultured lens epithelial cells by elemental MS
González-Iglesias H, Petrash C, Rodríguez-Menéndez S, García M, Álvarez L, Fernández-Vega Cueto L, Fernández B, Pereiro R, Sanz-Medel A, Coca-Prados M. Quantitative distribution of Zn, Fe and Cu in the human lens and study of the Zn–metallothionein redox system in cultured lens epithelial cells by elemental MS. Journal Of Analytical Atomic Spectrometry 2017, 32: 1746-1756. DOI: 10.1039/c6ja00431h.Peer-Reviewed Original ResearchLens epithelial cellsMolecular mass proteinsMass proteinsCultured human lens epithelial cellsHigh molecular mass proteinsEpithelial cellsCultured lens epithelial cellsHuman lens epithelial cellsHuman lensOxidative cellular damageLocalization of ZnEndogenous stressorsImportant enzymeMT proteinCellular distributionLens epithelial cell layerProteinRedox systemEpithelial cell layerElemental mass spectrometryCellular damageMetallothioneinOxidative stressHuman lensesCells
2014
The Blood-Aqueous Barrier in Health and Disease
Coca-Prados M. The Blood-Aqueous Barrier in Health and Disease. Journal Of Glaucoma 2014, 23: s36-s38. PMID: 25275903, DOI: 10.1097/ijg.0000000000000107.Peer-Reviewed Original ResearchConceptsBlood-aqueous barrierTight junctionsInflammatory ocular diseasesBlood-borne moleculesInner wall endotheliumInflammatory cellsBarrier dysfunctionVascular leakagePharmacologic agentsAnterior segmentOcular diseasesSchlemm's canalIris vasculatureCiliary processesEndothelial cellsJunctional complexesGap junctionsParacellular transportPhysiological changesDiseaseEyesCanalCellsApical junctional complex
2013
MRP4 and MRP5 in the ciliary epithelium of the eye and their potential role in regulating extracellular cAMP levels
Hotchkiss A, Pasumarthi K, Coca‐Prados M, Pelis R. MRP4 and MRP5 in the ciliary epithelium of the eye and their potential role in regulating extracellular cAMP levels. The FASEB Journal 2013, 27: 732.3-732.3. DOI: 10.1096/fasebj.27.1_supplement.732.3.Peer-Reviewed Original ResearchMultidrug resistance-associated proteinCAMP levelsExtracellular cAMP levelsCiliary epitheliumAqueous humor dynamicsResistance-associated proteinCiliary epithelial cell lineHuman ciliary bodyCE cellsEpithelial cell lineMRP inhibitorsCiliary bodyAdrenergic stimulationIntracellular cAMPIntracellular accumulationPotential roleCell linesIndomethacinFurosemideMRP4Cyclic nucleotidesMRP5EpitheliumIbuprofenCells
2010
Neuroendocrine Properties of the Ciliary Epithelium
Coca-Prados M. Neuroendocrine Properties of the Ciliary Epithelium. 2010, 107-112. DOI: 10.1016/b978-0-12-374203-2.00088-9.Peer-Reviewed Original ResearchCiliary epitheliumAqueous humorOcular hypotensive actionSame embryological originRetinal pigment epitheliumEye aqueous humorOcular ciliary epitheliumHypotensive actionAqueous humor secretionAnterior segmentEndocrine factorsPigment epitheliumNeuroendocrine propertiesEndocrine systemEmbryological originNeuroepithelial cellsEpitheliumAvascular tissueNeuropeptidesBioactive peptidesCellsCell communicationRetinaHormoneSecretion
2007
Characterization of the Intracellular Proteolytic Cleavage of Myocilin and Identification of Calpain II as a Myocilin-processing Protease*
Sánchez-Sánchez F, Martínez-Redondo F, Aroca-Aguilar JD, Coca-Prados M, Escribano J. Characterization of the Intracellular Proteolytic Cleavage of Myocilin and Identification of Calpain II as a Myocilin-processing Protease*. Journal Of Biological Chemistry 2007, 282: 27810-27824. PMID: 17650508, DOI: 10.1074/jbc.m609608200.Peer-Reviewed Original ResearchConceptsExtracellular calciumCalpain IICalcium-activated proteaseIntraocular pressureT cellsIntracellular proteolytic cleavageCalpain inhibitorsCalcium uptakeProteolytic cleavageCalpain inhibitor IVOlfactomedin-like domainCalpain IInhibitor IVMyocilinEndoplasmic reticulumIntracellular processingLumenRNA interference knockdownCalciumProteolytic processingCellsCulture mediumGlaucomaSubcellular fractionationEndoproteolytic processing
2003
CRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells
Kennedy BN, Li C, Ortego J, Coca-Prados M, Sarthy VP, Crabb JW. CRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells. Experimental Eye Research 2003, 76: 257-260. PMID: 12565814, DOI: 10.1016/s0014-4835(02)00308-1.Peer-Reviewed Original ResearchConceptsTranscriptional regulationBinding Protein FunctionsCell-specific expressionPromoter analysisProtein functionRepressor elementEnhancer elementsPromoter constructsReporter activityRetinal pigment epithelial cellsPigment epithelial cellsCiliary epitheliumEpithelial cellsVisual cycleRPE cellsBPRegulationRetinal pigment epitheliumCellsMüller cellsGenesMutationsRLBP1 geneEpitheliumWildtype
2001
PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells
Fleischhauer J, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2001, 281: c1614-c1623. PMID: 11600425, DOI: 10.1152/ajpcell.2001.281.5.c1614.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsCiliary epithelial cellsCl- channelsEpithelial cellsPE cellsCl- channel activityAction of nucleotidesProtein kinaseNonpigmented ciliary epithelial cellsSecond messengerCell sortingChannel activityATP activationElectronic cell sortingATPSequential cascadeChannel activationNucleotidesP2Y receptorsPatch clampingATP assayATP releaseCellsMicroM ATPCAMP
1998
Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume
Reeves R, Sanchez-Torres J, Coca-Prados M, Cammarata P. Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume. Current Eye Research 1998, 17: 861-869. PMID: 9746432, DOI: 10.1076/ceyr.17.9.861.5140.Peer-Reviewed Original ResearchConceptsBovine lens epithelial cellsCultured bovine lens epithelial cellsLens epithelial cellsHypertonic mediumChannel regulatory proteinRapid cellular swellingEpithelial cellsNorthern blot analysisTranscriptional regulationMyo-inositol effluxRegulatory proteinsMRNA expressionAnion channelCellular shrinkageCell volume changesCell volumeBlot analysisCell volume reductionExpression levelsMRNAChloride movementExpressionCellsMedium conditionsCellular swelling
1996
Crocin, safranal and picrocrocin from saffron (Crocus sativus L.) inhibit the growth of human cancer cells in vitro
Escribano J, Alonso G, Coca-Prados M, Fernández J. Crocin, safranal and picrocrocin from saffron (Crocus sativus L.) inhibit the growth of human cancer cells in vitro. Cancer Letters 1996, 100: 23-30. PMID: 8620447, DOI: 10.1016/0304-3835(95)04067-6.Peer-Reviewed Original ResearchConceptsExtract of saffronCytotoxic effectsCell growth inhibitionCancer therapeutic agentsHuman tumor cellsHuman cancer cellsPyknotic nucleiInhibitory growth effectTherapeutic agentsTumor cellsCancer cellsEthanolic extractApoptosis inductionCrocinGrowth inhibitionReduced cytoplasmCell growthCellsCrocetinCell shrinkageDosesHeLa cellsIdentification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium
Ortego J, Escribano J, Crabb J, Coca‐Prados M. Identification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1996, 66: 787-796. PMID: 8592153, DOI: 10.1046/j.1471-4159.1996.66020787.x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnimalsAqueous HumorBase SequenceCarboxypeptidase HCarboxypeptidasesCell LineChild, PreschoolChromograninsCiliary BodyEpithelial CellsEpitheliumFluorescent Antibody Technique, IndirectHumansMixed Function OxygenasesMolecular Sequence DataMultienzyme ComplexesNeurosecretory SystemsProteinsRatsConceptsNeuropeptide processing enzymesCarboxypeptidase ECiliary epithelial cellsOcular ciliary epitheliumSecretogranin IICell-cell communicationEpithelial cellsHuman ocular ciliary epitheliumCDNA clonesCell signalingNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsProcessing enzymesMolecular markersPeptidylglycine alphaPhorbol esterSpecialized groupSpecific probesMammalian eyeNeuroendocrine tissuesEnzymeCiliary epitheliumKey functionsCellsPolymerase chain reaction
1993
Studies on regulation of the ascorbic acid transporter in a cell line derived from rabbit non-pigmented ciliary epithelium
Delamere N, Coca-Prados M, Aggarwal S. Studies on regulation of the ascorbic acid transporter in a cell line derived from rabbit non-pigmented ciliary epithelium. Biochimica Et Biophysica Acta 1993, 1149: 102-108. PMID: 8391316, DOI: 10.1016/0005-2736(93)90030-4.Peer-Reviewed Original ResearchConceptsProtein kinase CKinase CAscorbate uptakeCell linesProtein kinase C inhibitorProtein kinase C activatorCultured cell linesPresence of staurosporineKinase C inhibitorAscorbic acid transporterNon-pigmented ciliary epitheliumAscorbate transporterUptake rateAcid transportersC activatorCiliary epitheliumC inhibitorNon-pigmented epithelial cellsPDBu treatmentUptake mechanismEpithelial cellsPDBu effectSodium gradientTransportersCells
1991
Mammalian ciliary-body epithelial cells in culture produce transforming growth factor-beta
Helbig H, Kittredge K, Coca-Prados M, Davis J, Palestine A, Nussenblatt R. Mammalian ciliary-body epithelial cells in culture produce transforming growth factor-beta. Graefe's Archive For Clinical And Experimental Ophthalmology 1991, 229: 84-87. PMID: 2004731, DOI: 10.1007/bf00172268.Peer-Reviewed Original Research
1988
Na+/H+ exchange regulates intracellular pH in a cell clone derived from bovine pigmented ciliary epithelium
Helbig H, Korbmacher C, Stumpff F, Coca‐Prados M, Wiederholt M. Na+/H+ exchange regulates intracellular pH in a cell clone derived from bovine pigmented ciliary epithelium. Journal Of Cellular Physiology 1988, 137: 384-389. PMID: 2848042, DOI: 10.1002/jcp.1041370225.Peer-Reviewed Original ResearchConceptsPigmented ciliary epitheliumMM amilorideAcid loadCiliary epitheliumCell clonesPH-sensitive absorbanceBicarbonate-free mediumRegulation of intracellularCiliary bodyAmilorideExtracellular protonsBovine ciliary bodyEpitheliumConfluent monolayersIntracellularCellsCellular metabolismSlow acidificationAlkalinizationPlastic coverslips
1987
Evidence that the α and α(+) isoforms of the catalytic subunit of (Na+,K+)-ATPase reside in distinct ciliary epithelial cells of the mammalian eye
Coca-Prados M, López-Briones L. Evidence that the α and α(+) isoforms of the catalytic subunit of (Na+,K+)-ATPase reside in distinct ciliary epithelial cells of the mammalian eye. Biochemical And Biophysical Research Communications 1987, 145: 460-466. PMID: 3036129, DOI: 10.1016/0006-291x(87)91343-x.Peer-Reviewed Original ResearchConceptsCatalytic subunitCiliary epithelial cellsEpithelial cellsNPE cellsCultured ciliary epithelial cellsBasolateral surfaceImmunoblot analysisCiliary processesIntact ciliary processesMammalian eyeATPasePE cellsSubunitsPolyclonal antibodiesCanine kidneyCellsAlpha formAlphaIndirect immunofluorescenceIsoformsProteinBovine eyes
1986
Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors.
Coca-Prados M, Wax M. Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 8754-8758. PMID: 3022303, PMCID: PMC387010, DOI: 10.1073/pnas.83.22.8754.Peer-Reviewed Original ResearchConceptsSimian virus 40Ciliary epithelial cellsSouthern blot analysisLarge tumor antigenHuman ciliary epithelial cellsEpithelial cellsFunctional conservationProtein phosphorylationDirect bindingCell DNAParent cellsBlot analysisCell proliferationCellular cAMP productionReceptor-mediated processEpithelial cell DNAViral DNAImmunoprecipitationDNACAMP productionSelective drugsCell clonesMembrane preparationsCellsReceptorsGrowth of non-pigmented ciliary epithelial cells in serum-free hormone-supplemented media
Coca-Prados M, Chatt G. Growth of non-pigmented ciliary epithelial cells in serum-free hormone-supplemented media. Experimental Eye Research 1986, 43: 617-629. PMID: 3792464, DOI: 10.1016/s0014-4835(86)80028-8.Peer-Reviewed Original ResearchConceptsNPE cellsClathrin-coated vesiclesCiliary epithelial cellsNerve growth factorEpithelial cellsNon-pigmented ciliary epithelial cellsCellular polarityGrowth factorCellular junctionsPrimary culturesCoated pitsArtificial substratesTransport activityCulture mediumMicrofilament bundlesCell growthSerum-free hormone-supplemented mediumJunctional complexesIntermediate filamentsCell proliferationAdjacent cellsCellsSerum-free culture medium
1983
Ciliary epithelia of the mammalian eye in cultured explants
Kondo K, Coca-Prados M, Sears M. Ciliary epithelia of the mammalian eye in cultured explants. Cell And Tissue Research 1983, 233: 629-638. PMID: 6627352, DOI: 10.1007/bf00212230.Peer-Reviewed Original ResearchConceptsPigment granulesCultured explantsDulbecco's Modified Eagle's MediumFocal junctionsColumnar cellsFetal bovine serumJunctional complexesCuboidal cellsMammalian eyeCiliary epitheliumChannel-like structuresEpithelial cellsPlastic culture dishesNonpigmented cellsExplantsCellsBovine serumUltrastructural characteristicsOutermost layerPlastic dishesModified Eagle MediumPigmented epitheliumBasement membraneEagle's mediumCulture dishes
1980
Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA.
Ford J, Coca-Prados M, Hsu M. Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA. Journal Of Biological Chemistry 1980, 255: 7544-7547. PMID: 6249806, DOI: 10.1016/s0021-9258(19)43861-1.Peer-Reviewed Original ResearchConceptsEukaryotic gene expressionSimian virus 40 DNAEukaryotic cellsEukaryotic DNASimian virus 40Gene expressionHost DNAViral genomeCell DNADNAMonkey kidney cellsIntracellular viral DNAEnzymatic analysisViral DNAKidney cellsGenomeCellsAcid hydrolysis procedureThymineExact quantitationDeaminationExpressionEnzymatic methodVivo
1979
Electron microscopic evidence for the circular form of RNA in the cytoplasm of eukaryotic cells
HSU M, COCA-PRADOS M. Electron microscopic evidence for the circular form of RNA in the cytoplasm of eukaryotic cells. Nature 1979, 280: 339-340. PMID: 460409, DOI: 10.1038/280339a0.Peer-Reviewed Original Research