2004
Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells
Al-Nakkash L, Iserovich P, Coca-Prados M, Yang H, Reinach P. Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells. The Journal Of Membrane Biology 2004, 201: 41-49. PMID: 15635811, DOI: 10.1007/s00232-004-0706-5.Peer-Reviewed Original ResearchConceptsCorneal epithelial cellsRabbit corneal epithelial cellsVolume-regulated anion channelsEpithelial cellsVoltage-dependent inhibitionHypotonic challengeVolume-activated chloride channelsRegulatory volume decrease (RVD) responseInactive phorbol ester analoguePresence of DIDSExtracellular calciumChloride currentsNiflumic acidRVD responseCell swellingDecrease responseNorthern blot analysisBlot analysisPhorbol dibutyratePhorbol ester analogsChloride channelsVACCNPPBMOsm mediumSV40-immortalized rabbit corneal epithelial cells
2001
Molecular Evidence That Human Ocular Ciliary Epithelium Expresses Components Involved in Phototransduction
Bertazolli-Filho R, Ghosh S, Huang W, Wollmann G, Coca-Prados M. Molecular Evidence That Human Ocular Ciliary Epithelium Expresses Components Involved in Phototransduction. Biochemical And Biophysical Research Communications 2001, 284: 317-325. PMID: 11394879, DOI: 10.1006/bbrc.2001.4970.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalArrestinBlotting, WesternCalcium-Binding ProteinsCattleCell LineCiliary BodyCyclic Nucleotide-Gated Cation ChannelsEpithelial CellsEye ProteinsFluorescent Antibody Technique, IndirectG-Protein-Coupled Receptor Kinase 1HippocalcinHumansImmunoblottingImmunohistochemistryIon ChannelsLight Signal TransductionLipoproteinsNerve Tissue ProteinsPhosphoric Diester HydrolasesProtein KinasesProtein SubunitsRecoverinRetinaReverse Transcriptase Polymerase Chain ReactionRhodopsinRNA, MessengerSequence Analysis, DNATransducinConceptsHuman ocular ciliary epitheliumCiliary epitheliumOcular ciliary epitheliumNPE cellsCiliary epithelial cell linePars plicata regionIntact ciliary epitheliumWestern blot analysisCultured NPE cellsEpithelial cell lineExpression of rhodopsinIndirect immunofluorescenceVisual arrestinHuman retinaMonoclonal antibodiesHuman ciliary epitheliumThree- to fourfoldEpitheliumRT-PCRPhotoreceptor cellsRhodopsin mRNABlot analysisCell linesRetinaRhodopsin kinase
1999
Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells
Sánchez-Torres J, Huang W, Civan M, Coca-Prados M. Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells. Current Eye Research 1999, 18: 408-416. PMID: 10435827, DOI: 10.1076/ceyr.18.6.408.5266.Peer-Reviewed Original ResearchConceptsOpen reading frameCiliary epithelial cellsPlasma membraneFusion proteinWestern blot analysisEpithelial cellsCellular distributionTag fusion proteinCandidate gene productsBlot analysisHuman nonpigmented ciliary epithelial cellsTotal cell extractsHypotonic treatmentHuman ciliary epithelial cellsMammalian cellsPolyclonal antibodiesReading frameGene productsNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsPerinuclear regionCellular localizationCell extractsCultured cellsProtein
1998
Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume
Reeves R, Sanchez-Torres J, Coca-Prados M, Cammarata P. Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume. Current Eye Research 1998, 17: 861-869. PMID: 9746432, DOI: 10.1076/ceyr.17.9.861.5140.Peer-Reviewed Original ResearchConceptsBovine lens epithelial cellsCultured bovine lens epithelial cellsLens epithelial cellsHypertonic mediumChannel regulatory proteinRapid cellular swellingEpithelial cellsNorthern blot analysisTranscriptional regulationMyo-inositol effluxRegulatory proteinsMRNA expressionAnion channelCellular shrinkageCell volume changesCell volumeBlot analysisCell volume reductionExpression levelsMRNAChloride movementExpressionCellsMedium conditionsCellular swelling
1997
Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells
ORTEGO J, ESCRIBANO J, COCA-PRADOS M. Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells. Experimental Eye Research 1997, 65: 289-299. PMID: 9268597, DOI: 10.1006/exer.1997.0333.Peer-Reviewed Original ResearchMeSH KeywordsAgedAlpha 1-AntitrypsinAlpha-MacroglobulinsBlotting, NorthernBlotting, WesternCarcinogensCathepsin DCathepsin KCathepsinsCell LineCiliary BodyCysteine EndopeptidasesDNA, ComplementaryEndopeptidasesEpithelial CellsGene ExpressionGlycoproteinsHumansMiddle AgedPolymerase Chain ReactionPrecipitin TestsPregnancy ProteinsProtease InhibitorsSerine Proteinase InhibitorsTetradecanoylphorbol AcetateConceptsODM-2 cellsPP5/TFPICiliary epithelial cellsComplementary DNARegulation of transcriptionProteinase inhibitorsEpithelial cellsNon-protein kinase CCathepsin DProtein kinase C activationKinase C activationPattern of expressionCiliary bodyImmunoprecipitation experimentsGene expressionNorthern analysisCultured ciliary epithelial cellsKinase CWestern blot analysisHuman ciliary epitheliumAlpha-phorbol didecanoatePhorbol esterC activationBlot analysisHuman proteinasesThe prostaglandin transporter is widely expressed in ocular tissues
Schuster V, Lu R, Coca-Prados M. The prostaglandin transporter is widely expressed in ocular tissues. Survey Of Ophthalmology 1997, 41: s41-s45. PMID: 9154275, DOI: 10.1016/s0039-6257(97)80006-9.Peer-Reviewed Original Research
1996
Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor.
Ortego J, Escribano J, Becerra S, Coca-Prados M. Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor. Investigative Ophthalmology & Visual Science 1996, 37: 2759-67. PMID: 8977492.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAqueous HumorBlotting, NorthernCattleCell LineCells, CulturedChromatography, AffinityCiliary BodyDNA PrimersEye ProteinsFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoenzyme TechniquesNerve Growth FactorsPigment Epithelium of EyePolymerase Chain ReactionProtein BiosynthesisProteinsRabbitsSerpinsTranscription, GeneticConceptsPigment epithelium-derived factorEpithelium-derived factorPolymerase chain reactionCiliary epitheliumCiliary bodyOcular tissuesBlot analysisHuman ciliary epitheliumNeuronal survival activityHuman ocular ciliary epitheliumRetinal pigment epitheliumNorthern blot analysisCell linesBovine ocular tissuesPars plicata regionOcular ciliary bodyOcular ciliary epitheliumWestern blot analysisFactor antibodyPEDF expressionTrophic factorsPigment epitheliumPEDF proteinPEDF transcriptAqueous humor
1995
Cell-specific expression of the human Na+,K(+)-ATPase beta 2 subunit isoform in the nonpigmented ciliary epithelium.
Coca-Prados M, Fernández-Cabezudo M, Sánchez-Torres J, Crabb J, Ghosh S. Cell-specific expression of the human Na+,K(+)-ATPase beta 2 subunit isoform in the nonpigmented ciliary epithelium. Investigative Ophthalmology & Visual Science 1995, 36: 2717-28. PMID: 7499094.Peer-Reviewed Original ResearchConceptsBeta 2 isoformNonpigmented ciliary epithelial cellsOcular tissuesCiliary epitheliumBeta 1NPE cell linesNonpigmented ciliary epitheliumNPE cellsHuman ocular tissuesSubunit isoformsBlot analysisATPase subunit isoformsSpecific anti-peptide antibodiesRestricted cellular distributionPolymerase chain reactionCiliary epithelial cellsBeta-subunit isoformsAlpha-subunit isoformsAnti-peptide antibodiesODM-2Polymerase chain reaction amplificationChain reaction amplificationHuman ciliary epitheliumCell-specific distributionPattern of expression
1989
Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells
Martin‐Vasallo P, Ghosh S, Coca‐Prados M. Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells. Journal Of Cellular Physiology 1989, 141: 243-252. PMID: 2553750, DOI: 10.1002/jcp.1041410203.Peer-Reviewed Original ResearchConceptsK-ATPase alpha subunit isoformsAlpha-subunit isoformsSubunit isoformsCultured cellsK-ATPase alpha subunitPE cellsExpression of NaBasolateral membrane domainsNorthern hybridization analysisNPE cell linesAlpha 2 mRNACell linesAlpha 3 isoformMembrane domainsAlpha 3 mRNAAlpha 1Alpha subunitCultured ciliary epithelial cellsWestern blot analysisHybridization analysisIsoformsCiliary epithelial cellsNPE layerBlot analysisK-ATPase
1986
Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors.
Coca-Prados M, Wax M. Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 8754-8758. PMID: 3022303, PMCID: PMC387010, DOI: 10.1073/pnas.83.22.8754.Peer-Reviewed Original ResearchConceptsSimian virus 40Ciliary epithelial cellsSouthern blot analysisLarge tumor antigenHuman ciliary epithelial cellsEpithelial cellsFunctional conservationProtein phosphorylationDirect bindingCell DNAParent cellsBlot analysisCell proliferationCellular cAMP productionReceptor-mediated processEpithelial cell DNAViral DNAImmunoprecipitationDNACAMP productionSelective drugsCell clonesMembrane preparationsCellsReceptors