2016
Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection
Wiśniewski M, Jaros S, Bąska P, Cappello M, Długosz E, Wędrychowicz H. Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection. Experimental Parasitology 2016, 163: 1-7. PMID: 26795262, DOI: 10.1016/j.exppara.2016.01.006.Peer-Reviewed Original ResearchConceptsA. ceylanicum infectionAncylostoma ceylanicum infectionHookworm speciesWorm burden reductionsSyrian golden hamstersProtective immunityDNA vaccineGut mucosaGastrointestinal tractIntestinal nematodesGolden hamstersBurden reductionAdult wormsHemoglobin digestionEgg countsImmunizationVaccineInfectionHamstersMetalloproteasesMucosaHookwormBloodTractImmunity
2013
Hookworm Infection among School Age Children in Kintampo North Municipality, Ghana: Nutritional Risk Factors and Response to Albendazole Treatment
Humphries D, Simms BT, Davey D, Otchere J, Quagraine J, Terryah S, Newton S, Berg E, Harrison LM, Boakye D, Wilson M, Cappello M. Hookworm Infection among School Age Children in Kintampo North Municipality, Ghana: Nutritional Risk Factors and Response to Albendazole Treatment. American Journal Of Tropical Medicine And Hygiene 2013, 89: 540-548. PMID: 23836564, PMCID: PMC3771297, DOI: 10.4269/ajtmh.12-0605.Peer-Reviewed Original ResearchConceptsKintampo North MunicipalityHookworm infectionCure rateSchool-aged childrenModest cure ratesSingle-dose albendazoleNutritional risk factorsLevels of antibodiesYears of ageAge childrenMalaria parasitemiaAlbendazole treatmentRisk factorsChildren 6InfectionInfection statusHealth careSignificant predictorsAnimal foodsHookwormAlbendazoleFurther investigationAgeChildrenLow consumption
2012
Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum
Davey D, Manickam N, Simms BT, Harrison LM, Vermeire JJ, Cappello M. Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum. Experimental Parasitology 2012, 133: 243-249. PMID: 23232252, PMCID: PMC3580025, DOI: 10.1016/j.exppara.2012.11.010.Peer-Reviewed Original ResearchConceptsExperimental infectionThird-stage hookworm larvaeParasite-specific IgAHumoral immune responseStudy of pathogenesisResource-limited countriesHuman hookworm diseaseHookworm infectionLifelong susceptibilityPrimary infectionImmune responseProlonged susceptibilitySubsequent challengeAntibody productionVaccine developmentHookworm larvaeHookworm diseaseIntestinal nematodesInfectionPathological effectsRepeated exposureAncylostoma ceylanicumContinued susceptibilityMajor causeMediate resistance
2010
CD4+ T cells mediate mucosal and systemic immune responses to experimental hookworm infection
DONDJI B, SUN T, BUNGIRO RD, VERMEIRE JJ, HARRISON LM, BIFULCO C, CAPPELLO M. CD4+ T cells mediate mucosal and systemic immune responses to experimental hookworm infection. Parasite Immunology 2010, 32: 406-413. PMID: 20500671, PMCID: PMC3241967, DOI: 10.1111/j.1365-3024.2010.01204.x.Peer-Reviewed Original ResearchConceptsT cellsImmune responseHookworm infectionHost cellular immune responseExperimental hookworm infectionT-cell depletionMesenteric lymph nodesSystemic immune responsesCellular immune responsesHumoral immune responseIntestinal nematode infectionsIntestinal worm burdenResource-limited countriesHookworm antigensLymph nodesSevere anemiaIntraperitoneal injectionLymphocyte proliferationHookworm Ancylostoma ceylanicumIsotype controlDisease pathogenesisWorm burdenSyrian hamstersAncylostoma ceylanicumEffector role
2008
Mucosal antibody responses in experimental hookworm infection
BUNGIRO RD, SUN T, HARRISON LM, SHOEMAKER CB, CAPPELLO M. Mucosal antibody responses in experimental hookworm infection. Parasite Immunology 2008, 30: 293-303. PMID: 18312503, DOI: 10.1111/j.1365-3024.2008.01023.x.Peer-Reviewed Original ResearchConceptsMucosal IgA responsesSystemic immune responsesIgA responsesImmune responseHookworm infectionInfected hamstersRobust systemic immune responseAncylostoma ceylanicum infectionExperimental hookworm infectionFecal IgA responsesParasite-specific IgAMucosal immune responsesMucosal antibody responsesIntestinal worm burdenExcretory-secretory productsMucosal antibodiesProtective immunityChallenge infectionSerum IgGAntibody responsePrimary infectionSecretory IgAHamster modelIntestinal mucosaWorm burden
2005
Detection of excretory/secretory coproantigens in experimental hookworm infection.
Bungiro RD, Cappello M. Detection of excretory/secretory coproantigens in experimental hookworm infection. American Journal Of Tropical Medicine And Hygiene 2005, 73: 915-20. PMID: 16282303, DOI: 10.4269/ajtmh.2005.73.915.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayMolecular host-parasite interactionsFuture diagnostic methodsExcretory/secretoryFecal extractsImmunosorbent assayA. ceylanicum infectionExperimental hookworm infectionIntestinal worm burdenRabbit polyclonal IgG antibodyPolyclonal IgG antibodiesES proteinsDiagnostic methodsHookworm antigensDetectionHookworm infectionIgG antibodiesStool samplesWorm burdenDay 17Hamster serumCoproantigenFaecal eggMug/Degrees C.
2004
Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms
Bungiro RD, Solis CV, Harrison LM, Cappello M. Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms. Infection And Immunity 2004, 72: 2203-2213. PMID: 15039344, PMCID: PMC375217, DOI: 10.1128/iai.72.4.2203-2213.2004.Peer-Reviewed Original ResearchMeSH KeywordsAdministration, OralAmino Acid SequenceAncylostomaAncylostomiasisAnimalsAntibodies, HelminthAntigens, HelminthBase SequenceCloning, MolecularCricetinaeHelminth ProteinsImmunizationImmunization, PassiveInjections, SubcutaneousMesocricetusMolecular Sequence DataRecombinant ProteinsSequence Analysis, DNAVaccinesConceptsAdult worm RNANovel immunoreactive proteinsCDNA predictsImmunization of hamstersES productsEnzyme-linked immunosorbentProtein 2Immunoreactive proteinExcretory-secretory productsHookworm anaemiaOral immunizationGlobal morbidityReverse transcription-PCRInfected hamstersInduced antibodiesHigh-pressure liquid chromatographyReverse-phase high-pressure liquid chromatographyNormal serumAdult hookwormsComplete recoveryVaccinationHyperimmune rabbitAnemiaImmunizationAdult wormsMolecular Characterization of Ancylostoma ceylanicum Kunitz-Type Serine Protease Inhibitor: Evidence for a Role in Hookworm-Associated Growth Delay
Chu D, Bungiro RD, Ibanez M, Harrison LM, Campodonico E, Jones BF, Mieszczanek J, Kuzmic P, Cappello M. Molecular Characterization of Ancylostoma ceylanicum Kunitz-Type Serine Protease Inhibitor: Evidence for a Role in Hookworm-Associated Growth Delay. Infection And Immunity 2004, 72: 2214-2221. PMID: 15039345, PMCID: PMC375216, DOI: 10.1128/iai.72.4.2214-2221.2004.Peer-Reviewed Original ResearchConceptsGrowth delayAdult hookwormsIron deficiency anemiaPolyclonal immunoglobulin GDeficiency anemiaHookworm infectionReverse transcription-PCRKunitz-type serine protease inhibitorImmunohistochemistry studiesBroad-spectrum inhibitorPartial protectionMajor causeImmunoglobulin GSerine protease inhibitorIntestinal proteasesThird-stage larvaeTranscription-PCRProtease inhibitorsAnemiaHookwormVivo roleMalnutritionInhibitorsInfected hostPancreatic elastase
2003
Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum
Del Valle A, Jones BF, Harrison LM, Chadderdon RC, Cappello M. Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum. Molecular And Biochemical Parasitology 2003, 129: 167-177. PMID: 12850261, DOI: 10.1016/s0166-6851(03)00121-x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntibodies, HelminthChromatography, High Pressure LiquidCloning, MolecularCollagenDNA, ComplementaryFibrinogenHelminth ProteinsImmunoglobulin GImmunohistochemistryIntegrin alpha2beta1Molecular Sequence DataPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexRecombinant ProteinsSpectrometry, Mass, Electrospray IonizationConceptsIron deficiency anemiaCell surface integrin receptorsIdentical N-terminalAmino acid sequence homologyAmino acid sequenceSingle gene productSurface integrin receptorsDeficiency anemiaMammalian hemostasisCorresponding native proteinsCDNA correspondingSignificant homologyMolecular cloningSimilar molecular massSecreted proteinsGene productsSequence homologyAcid sequenceInhibitor proteinHPI proteinVaccine-based strategiesNative proteinN-terminalAdult Ancylostoma caninumAdult stageOral Transfer of Adult Ancylostoma ceylanicum Hookworms into Permissive and Nonpermissive Host Species
Bungiro RD, Anderson BR, Cappello M. Oral Transfer of Adult Ancylostoma ceylanicum Hookworms into Permissive and Nonpermissive Host Species. Infection And Immunity 2003, 71: 1880-1886. PMID: 12654804, PMCID: PMC152085, DOI: 10.1128/iai.71.4.1880-1886.2003.Peer-Reviewed Original ResearchConceptsBALB/c miceHigh-dose groupHumoral immune responseCD-1 miceLong-term survivalRecovery of parasitesExcretory-secretory productsHemoglobin levelsPrechallenge levelsC57BL/6 miceHookworm infectionC miceWeanling hamstersOral infectionImmune responseMurine modelHookworm larvaeSyrian hamstersMouse strainsTypical infectionsInfectionPrior exposureMiceAdult wormsOral transfer
2001
Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic
Harrison L, Córdova J, Cappello M. Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic. Molecular And Biochemical Parasitology 2001, 115: 101-107. PMID: 11377744, DOI: 10.1016/s0166-6851(01)00276-6.Peer-Reviewed Original ResearchConceptsPolyclonal IgGAnticoagulant activityFuture immunization strategiesGastrointestinal blood lossIron deficiency anemiaPartial thromboplastin timeFactor Xa activityHuman coagulation factor XaBlood lossDeficiency anemiaHookworm infectionThromboplastin timeXa activityAmphidial glandsAdult Ancylostoma caninum hookwormsCoagulation factor XaAdult hookwormsImmunization strategiesA. ceylanicumIgGMajor causeHookwormSerine protease inhibitorAdult wormsViable targetMitigation of Hookworm Disease by Immunization with Soluble Extracts of Ancylostoma ceylanicum
Bungiro R, Greene J, Kruglov E, Cappello M. Mitigation of Hookworm Disease by Immunization with Soluble Extracts of Ancylostoma ceylanicum. The Journal Of Infectious Diseases 2001, 183: 1380-1387. PMID: 11294670, DOI: 10.1086/319867.Peer-Reviewed Original ResearchConceptsHookworm infectionMajor clinical sequelaeHuman vaccine developmentCause of anemiaSyrian golden hamstersAdult worm burdenGlobal control effortsClinical sequelaeActive vaccinationPrimary infectionWorm burdenAdult hookwormsGrowth delayVaccine developmentHookworm diseaseGolden hamstersPooled seraAncylostoma ceylanicumPartial protectionAnemiaWeight lossAbsence of reductionInfectionAdult parasitesHamsters
1988
Induction of resistance to Schistosoma mansoni by immunization with subfractions of worms.
Knopf P, Goldberg M, Grossi C, Cappello M, Coulter S. Induction of resistance to Schistosoma mansoni by immunization with subfractions of worms. American Journal Of Tropical Medicine And Hygiene 1988, 38: 515-28. PMID: 3152780, DOI: 10.4269/ajtmh.1988.38.515.Peer-Reviewed Original ResearchConceptsInfection 3 weeksSchistosoma mansoni infectionExposure of ratsInduction of resistanceGlutaraldehyde-fixed parasitesLive infectionMansoni infectionBooster injectionChallenge infectionNaive ratsImmunization protocolDifferent adjuvantsLow dosesDose sizeRatsSchistosoma mansoniPartial protectionInfectionMiceInjectionWeeksInductionSubfractionsParasitesBuffered saline