2019
Discovery and Contribution of Nontypeable Haemophilus influenzae NTHI1441 to Human Respiratory Epithelial Cell Invasion
Ahearn C, Kirkham C, Chaves L, Kong Y, Pettigrew M, Murphy T. Discovery and Contribution of Nontypeable Haemophilus influenzae NTHI1441 to Human Respiratory Epithelial Cell Invasion. Infection And Immunity 2019, 87: 10.1128/iai.00462-19. PMID: 31427451, PMCID: PMC6803334, DOI: 10.1128/iai.00462-19.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary diseaseRespiratory epithelial cellsEnzyme-linked immunosorbent assayEpithelial cellsAirways of adultsObstructive pulmonary diseaseCell invasionHuman respiratory epithelial cellsNontypeable Haemophilus influenzaeCell enzyme-linked immunosorbent assayAlveolar epithelial cellsAcute exacerbationLower airwaysPulmonary diseaseSerum IgGNTHi strainsTherapeutic interventionsHaemophilus influenzaeHuman bronchialSurface-exposed epitopesEpithelial cell invasionIsogenic knockout mutantsNTHiImmunosorbent assayIntracellular survival
2016
Association of sputum microbiota profiles with severity of community-acquired pneumonia in children
Pettigrew MM, Gent JF, Kong Y, Wade M, Gansebom S, Bramley AM, Jain S, Arnold SL, McCullers JA. Association of sputum microbiota profiles with severity of community-acquired pneumonia in children. BMC Infectious Diseases 2016, 16: 317. PMID: 27391033, PMCID: PMC4939047, DOI: 10.1186/s12879-016-1670-4.Peer-Reviewed Original ResearchConceptsCommunity-acquired pneumoniaCAP severityMicrobiota profilesClinical courseRespiratory microbiotaPediatric community-acquired pneumoniaIntensive care unit admissionNasopharyngeal/oropharyngeal samplesCare unit admissionOdds of lengthInduced sputum samplesRespiratory tract microbiotaOP samplesRibosomal RNA sequencingUnit admissionDecreased oddsSputum samplesChildren 6Oropharyngeal samplesMicrobiota influenceChildren 5Logistic regressionSputumSeverityPneumonia
2012
Upper Respiratory Tract Microbial Communities, Acute Otitis Media Pathogens, and Antibiotic Use in Healthy and Sick Children
Pettigrew MM, Laufer AS, Gent JF, Kong Y, Fennie KP, Metlay JP. Upper Respiratory Tract Microbial Communities, Acute Otitis Media Pathogens, and Antibiotic Use in Healthy and Sick Children. Applied And Environmental Microbiology 2012, 78: 6262-6270. PMID: 22752171, PMCID: PMC3416608, DOI: 10.1128/aem.01051-12.Peer-Reviewed Original ResearchConceptsAcute otitis mediaAntibiotic useS. pneumoniaeHealthy childrenM. catarrhalisH. influenzaeAcute otitis media pathogensConcurrent acute otitis mediaUpper respiratory tract infectionRespiratory tract infectionsOtitis media pathogensUpper respiratory tractCross-sectional studyYears of ageAOM pathogensTract infectionsOtitis mediaMoraxella catarrhalisRespiratory tractReal-time PCRNasal swabsSick childrenPrevention strategiesHaemophilus influenzaeStreptococcus pneumoniae
2011
Streptococcus pneumoniae Serotype 6C: an Intra- and Interclonal Complex Comparison
Thomas JC, Kong Y, Sabharwal V, Pelton SI, Pettigrew MM. Streptococcus pneumoniae Serotype 6C: an Intra- and Interclonal Complex Comparison. Journal Of Bacteriology 2011, 193: 3409-3410. PMID: 21551297, PMCID: PMC3133277, DOI: 10.1128/jb.05079-11.Peer-Reviewed Original Research
2010
Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine
Laufer AS, Thomas JC, Figueira M, Gent JF, Pelton SI, Pettigrew MM. Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine. Vaccine 2010, 28: 2450-2457. PMID: 20067753, PMCID: PMC2851619, DOI: 10.1016/j.vaccine.2009.12.078.Peer-Reviewed Original ResearchConceptsSerotype 19ASerotype 15B/CChinchilla otitis media modelMiddle ear infectionOtitis media modelDisease prevention effortsStreptococcus pneumoniae isolatesExperimental otitis mediaStreptococcus pneumoniae serotypesQuantitative PCRConjugate vaccineOtitis mediaEar infectionsPneumoniae isolatesVaccine formulationsPneumoniae serotypesSame sequence typeVaccine targetsPrevention effortsPathogenic potentialDifferent serotypesSequence typesSerotypesIsolatesVaccine
2009
Molecular Epidemiology of Pediatric Pneumococcal Empyema from 2001 to 2007 in Utah
Byington CL, Hulten KG, Ampofo K, Sheng X, Pavia AT, Blaschke AJ, Pettigrew M, Korgenski K, Daly J, Mason EO. Molecular Epidemiology of Pediatric Pneumococcal Empyema from 2001 to 2007 in Utah. Journal Of Clinical Microbiology 2009, 48: 520-525. PMID: 20018815, PMCID: PMC2815589, DOI: 10.1128/jcm.01200-09.Peer-Reviewed Original ResearchConceptsUtah childrenPCV-7Nonvaccine serotypesPneumococcal empyemaMolecular epidemiologyStreptococcus pneumoniae serotype 1Pneumococcal conjugate vaccineSerotype 1Sequence typesCommon sequence typesEmpyema ratesSerotype replacementConjugate vaccineSerotype switchReplacement diseasePPE casesEmpyemaChildrenSerotypesHigh rateEpidemiologyLicensureMLSTVaccineIncidence
2004
An array of diverse microbial genomes
Pettigrew MM. An array of diverse microbial genomes. Trends In Biotechnology 2004, 22: 491-493. PMID: 15450738, DOI: 10.1016/j.tibtech.2004.08.008.Peer-Reviewed Original ResearchMeSH KeywordsBacteriaDNA, BacterialGenetic VariationGenome, BacterialGenomic LibraryGenomicsOligonucleotide Array Sequence AnalysisDiversity and sharing of Haemophilus influenzae strains colonizing healthy children attending day-care centers
FARJO RS, FOXMAN B, PATEL MJ, ZHANG L, PETTIGREW MM, MCCOY SI, MARRS CF, GILSDORF JR. Diversity and sharing of Haemophilus influenzae strains colonizing healthy children attending day-care centers. The Pediatric Infectious Disease Journal 2004, 23: 41-46. PMID: 14743045, DOI: 10.1097/01.inf.0000106981.89572.d1.Peer-Reviewed Original ResearchMeSH KeywordsCarrier StateChild Day Care CentersChild, PreschoolCohort StudiesColony Count, MicrobialDNA, BacterialElectrophoresis, Gel, Pulsed-FieldFemaleGenotypeHaemophilus InfectionsHaemophilus influenzaeHumansIncidenceMaleMass ScreeningMichiganOtitis MediaPolymerase Chain ReactionReference ValuesRisk AssessmentSerotypingConceptsDay care centersH. influenzaeHealthy childrenH. influenzae organismsAcute otitis mediaH. influenzae colonizationH. influenzae strainsUnique strainsEnterobacterial repetitive intergenic consensus PCRThroat culturesOtitis mediaInitial screening techniqueNasopharyngeal colonizationPrevalence ratesColonizing strainsHaemophilus influenzaeHeterogeneity of strainInfluenzaeConsensus PCRRate of colonizationChildrenField gel electrophoresisSame strainPathogenic microorganismsEvidence of sharing
2002
Identification of the Lipooligosaccharide Biosynthesis Gene lic2B as a Putative Virulence Factor in Strains of Nontypeable Haemophilus influenzae That Cause Otitis Media
Pettigrew M, Foxman B, Marrs C, Gilsdorf J. Identification of the Lipooligosaccharide Biosynthesis Gene lic2B as a Putative Virulence Factor in Strains of Nontypeable Haemophilus influenzae That Cause Otitis Media. Infection And Immunity 2002, 70: 3551-3556. PMID: 12065495, PMCID: PMC128108, DOI: 10.1128/iai.70.7.3551-3556.2002.Peer-Reviewed Original ResearchConceptsMiddle ear isolatesNT H. influenzaeOtitis mediaH. influenzaeEar isolatesThroat isolatesHaemophilus influenzaeMiddle earAcute otitis mediaDay careNontypeable Haemophilus influenzaeVirulence factorsSpecific virulence factorsType B isolatesOM pathogenesisPutative virulence factorsH. influenzae geneImportant causeClinical isolatesPathogenic processesInfluenzaeB isolatesAcid levelsAmino acid levelsNontypeable