2013
Anr and Its Activation by PlcH Activity in Pseudomonas aeruginosa Host Colonization and Virulence
Jackson A, Gross M, Daniels E, Hampton T, Hammond J, Vallet-Gely I, Dove S, Stanton B, Hogan D. Anr and Its Activation by PlcH Activity in Pseudomonas aeruginosa Host Colonization and Virulence. Journal Of Bacteriology 2013, 195: 3093-3104. PMID: 23667230, PMCID: PMC3697539, DOI: 10.1128/jb.02169-12.Peer-Reviewed Original ResearchConceptsANR activityGlycine betainePlcH activityBiofilm formationHemolytic phospholipase CEnhanced biofilm formationGlucose-containing mediumP. aeruginosa PAO1Host colonizationLow oxygen tensionWild typeAbiotic surfacesMicroarray analysisAeruginosa PAO1Epithelial cell monolayersAbundant lipidsLines of evidenceAddition of cholinePhospholipase CCell membraneVirulenceBacterial metabolismProtein levelsMutantsRespiratory activity
2012
Prevalence of Streptococci and Increased Polymicrobial Diversity Associated with Cystic Fibrosis Patient Stability
Filkins L, Hampton T, Gifford A, Gross M, Hogan D, Sogin M, Morrison H, Paster B, O'Toole G. Prevalence of Streptococci and Increased Polymicrobial Diversity Associated with Cystic Fibrosis Patient Stability. Journal Of Bacteriology 2012, 194: 4709-4717. PMID: 22753064, PMCID: PMC3415522, DOI: 10.1128/jb.00566-12.Peer-Reviewed Original ResearchConceptsDiverse microbial communitiesHigher bacterial diversityCystic fibrosis lung environmentQuantitative PCRGenomic DNA samplesCystic fibrosis patientsMicrobial communitiesRRNA geneBacterial communitiesBacterial diversityOverall diversityTaxonomic profilesGroup speciesMicroarray analysisDominant organismsPseudomonas aeruginosaPatient stabilityPredominant generaFibrosis patientsSpeciesHypervariable regionSpecies of StreptococcusCurrent dogmaStable lung diseaseDiversity
2011
Hemolytic Phospholipase C Inhibition Protects Lung Function during Pseudomonas aeruginosa Infection
Wargo M, Gross M, Rajamani S, Allard J, Lundblad L, Allen G, Vasil M, Leclair L, Hogan D. Hemolytic Phospholipase C Inhibition Protects Lung Function during Pseudomonas aeruginosa Infection. American Journal Of Respiratory And Critical Care Medicine 2011, 184: 345-354. PMID: 21562128, PMCID: PMC3175536, DOI: 10.1164/rccm.201103-0374oc.Peer-Reviewed Original ResearchConceptsLung functionP. aeruginosa infectionAeruginosa infectionSignificant lung function impairmentLung function impairmentLung function declineBronchoalveolar lavage fluidPseudomonas aeruginosa infectionChronic lung infectionVirulence factorsP. aeruginosaP. aeruginosa virulence factorsPulmonary surfactant functionSingle virulence factorFunction declineMechanical ventilationFunction impairmentLavage fluidRespiratory endpointsSurfactant dysfunctionComputer-controlled ventilatorLung infectionAeruginosa virulence factorsStrain infectionLung physiology
2008
GbdR Regulates Pseudomonas aeruginosa plcH and pchP Transcription in Response to Choline Catabolites
Wargo M, Ho T, Gross M, Whittaker L, Hogan D. GbdR Regulates Pseudomonas aeruginosa plcH and pchP Transcription in Response to Choline Catabolites. Infection And Immunity 2008, 77: 1103-1111. PMID: 19103776, PMCID: PMC2643652, DOI: 10.1128/iai.01008-08.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAraC Transcription FactorBetaineCholineDNA Mutational AnalysisElectrophoretic Mobility Shift AssayGene Expression Regulation, BacterialMaleMiceMice, Inbred C57BLPhosphoric Monoester HydrolasesPhosphorylcholinePromoter Regions, GeneticPseudomonas aeruginosaPseudomonas InfectionsReverse Transcriptase Polymerase Chain ReactionSarcosineTranscription, GeneticTransferases (Other Substituted Phosphate Groups)ConceptsGlycine betainePlcH activityAraC family transcription factorElectrophoretic mobility shift assaysFamily transcription factorsEukaryotic cell membranesHemolytic phospholipase CMobility shift assaysP. aeruginosa virulenceProtein fusionsPromoter mappingTranscriptional activationTranscription factorsGbdRPhosphorylcholine phosphatasePromoter sequencesShift assaysGene expressionPromoter mutantsFeedback inductionMutantsTranscriptionPhospholipase CPrimary regulatorCell membrane