2016
Ral-Arf6 crosstalk regulates Ral dependent exocyst trafficking and anchorage independent growth signalling
Pawar A, Meier JA, Dasgupta A, Diwanji N, Deshpande N, Saxena K, Buwa N, Inchanalkar S, Schwartz MA, Balasubramanian N. Ral-Arf6 crosstalk regulates Ral dependent exocyst trafficking and anchorage independent growth signalling. Cellular Signalling 2016, 28: 1225-1236. PMID: 27269287, PMCID: PMC4973806, DOI: 10.1016/j.cellsig.2016.05.023.Peer-Reviewed Original ResearchMeSH KeywordsADP-Ribosylation Factor 6ADP-Ribosylation FactorsAnimalsCell AdhesionCell Line, TumorCell ProliferationCell Transformation, NeoplasticEmbryo, MammalianExocytosisExtracellular Signal-Regulated MAP KinasesFibroblastsHumansMembrane MicrodomainsMiceProtein TransportRal GTP-Binding ProteinsSignal TransductionConceptsARF6 activationRegulatory crosstalkActive RalIntegrin-dependent regulationOncogenic H-RasIndependent growthIntegrin-dependent activationAdhesion-dependent pathwayKey downstream mediatorSmall GTPase RalActive RalABladder cancer T24 cellsDependent traffickingRal functionIsoforms functionRaft microdomainsOncogenic RasPlasma membraneDependent regulationH-RasArf6Dependent activationDependent pathwayDownstream mediatorVital mediators
2007
A fluorescence resonance energy transfer activation sensor for Arf6
Hall B, McLean MA, Davis K, Casanova JE, Sligar SG, Schwartz MA. A fluorescence resonance energy transfer activation sensor for Arf6. Analytical Biochemistry 2007, 374: 243-249. PMID: 18162163, PMCID: PMC2277471, DOI: 10.1016/j.ab.2007.11.032.Peer-Reviewed Original ResearchConceptsMembrane targetingPlatelet-derived growth factorARF6 activationFluorescent proteinGreen fluorescent protein derivativesNormal membrane targetingRas family GTPasesDownstream effector proteinsSmall GTPase Arf6Small GTPase activationFluorescent reporter proteinFluorescent protein derivativesEffector proteinsExchange factorGTPase Arf6Effector domainReporter proteinGTPase activationRac activationN-terminusArf6Intact cellsCell migrationNormal regulationProteinArf6 and microtubules in adhesion-dependent trafficking of lipid rafts
Balasubramanian N, Scott DW, Castle JD, Casanova JE, Schwartz MA. Arf6 and microtubules in adhesion-dependent trafficking of lipid rafts. Nature Cell Biology 2007, 9: 1381-1391. PMID: 18026091, PMCID: PMC2715295, DOI: 10.1038/ncb1657.Peer-Reviewed Original ResearchConceptsPlasma membraneLipid raftsAnchorage-dependent signalingArf6-dependent mannerCaveolin-dependent internalizationSmall GTPase Arf6Microtubule-dependent traffickingIntegrin-mediated adhesionRecycling endosomesGTPase Arf6Membrane raftsDetachment of cellsCell spreadingF-actinRaftsArf6MicrotubulesEndosomesRac1TraffickingMembraneCellsEndocytosisSignalingPathway