1987
Nonpolarized secretion of truncated forms of the influenza hemagglutinin and the vesicular stomatitus virus G protein from MDCK cells.
Gonzalez A, Rizzolo L, Rindler M, Adesnik M, Sabatini D, Gottlieb T. Nonpolarized secretion of truncated forms of the influenza hemagglutinin and the vesicular stomatitus virus G protein from MDCK cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 3738-3742. PMID: 3035552, PMCID: PMC304951, DOI: 10.1073/pnas.84.11.3738.Peer-Reviewed Original ResearchConceptsApical plasma membrane domainMDCK cellsPlasma membrane domainsPolarity of secretionVirus G proteinViral glycoproteinsVesicular stomatitus virusMembrane anchoringCorresponding genesMembrane domainsCytoplasmic segmentNonpolarized fashionG proteinsEndogenous glycoproteinsApical surfaceProteinEpithelial cellsGlycoprotein GG glycoproteinGlycoproteinConfluent monolayersCellsInfluenza hemagglutininCDNAGenes
1986
Secretion of endogenous and exogenous proteins from polarized MDCK cell monolayers.
Gottlieb T, Beaudry G, Rizzolo L, Colman A, Rindler M, Adesnik M, Sabatini D. Secretion of endogenous and exogenous proteins from polarized MDCK cell monolayers. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 2100-2104. PMID: 3083413, PMCID: PMC323238, DOI: 10.1073/pnas.83.7.2100.Peer-Reviewed Original ResearchConceptsMDCK cellsCellular endomembrane systemEpithelial cell polarityMembrane-associated precursorEndogenous productsMature secretory productCell polarityPolarized MDCK cell monolayersEndomembrane systemAlpha 2u-globulinCorresponding genesSecretory pathwayMDCK cell monolayersRegulated pathwaySecretory productsImmunoglobulin kappa chainConstitutive secretionExogenous proteinsProteolytic cleavageSorting mechanismBasolateral surfaceApical surfaceProteinVectorial secretionCell of origin