2008
Dolichyl-Phosphate-Glucose Is Used To Make O-Glycans on Glycoproteins of Trichomonas vaginalis▿ †
Grabińska K, Ghosh S, Guan Z, Cui J, Raetz C, Robbins P, Samuelson J. Dolichyl-Phosphate-Glucose Is Used To Make O-Glycans on Glycoproteins of Trichomonas vaginalis▿ †. MSphere 2008, 7: 1344-1351. PMID: 18552282, PMCID: PMC2519777, DOI: 10.1128/ec.00061-08.Peer-Reviewed Original ResearchConceptsDol-P-Man synthase activityO-glycansSynthase activityNumerous gene duplicationsSaccharomyces cerevisiae membranesDolichyl-P-mannose synthaseN-glycosylation defectGene duplicationHuge genomeDPM1 geneNumerous genesGlycosylation defectsDolichyl-PPGene productsRecombinant proteinsSugar donorIsoprene unitsEndogenous glycoproteinsGenesDolichyl phosphateMembraneGlycoproteinFirst demonstrationProtistsALG5
1997
Polyprenol formation in the yeast Saccharomyces cerevisiae: effect of farnesyl diphosphate synthase overexpression
Szkopińska A, Grabińska K, Delourme D, Karst F, Rytka J, Palamarczyk G. Polyprenol formation in the yeast Saccharomyces cerevisiae: effect of farnesyl diphosphate synthase overexpression. Journal Of Lipid Research 1997, 38: 962-968. PMID: 9186913, DOI: 10.1016/s0022-2275(20)37220-5.Peer-Reviewed Original ResearchConceptsWild-type yeastType yeastSaccharomyces cerevisiaeYeast Saccharomyces cerevisiaeEffects of farnesylSynthesis of dolicholSame genetic backgroundHand overexpressionErg mutantsFPP synthaseSqualene synthase activitySynthesis of polyprenolsExogenous FPPMevalonate pathwayMutantsGenetic backgroundYeastOverexpressionSynthase overexpressionSynthase activityCerevisiaeFarnesylGenesSynthasePolyprenols