Altered V-ATPase expression in renal intercalated cells isolated from B1 subunit-deficient mice by fluorescence-activated cell sorting
Vedovelli L, Rothermel JT, Finberg KE, Wagner CA, Azroyan A, Hill E, Breton S, Brown D, Păunescu T. Altered V-ATPase expression in renal intercalated cells isolated from B1 subunit-deficient mice by fluorescence-activated cell sorting. American Journal Of Physiology. Renal Physiology 2012, 304: f522-f532. PMID: 23269648, PMCID: PMC3602708, DOI: 10.1152/ajprenal.00394.2012.Peer-Reviewed Original ResearchConceptsV-ATPase expressionVacuolar proton-pumping ATPaseProtein levelsV-ATPase subunitsFluorescence-assisted cellProton-pumping ATPaseV-ATPase AWestern blotFluorescence-activated cell sortingV-ATPasesSubunit protein levelsE1 subunitSubunit promoterQuantitative Western blotApical membraneEGFP expressionH subunitAcid-base homeostasisEGFPSubunitsCell sortingExpressionCytosol fractionCellsMembrane