Energetics of S-Adenosylmethionine Synthetase Catalysis †
McQueney M, Anderson K, Markham G. Energetics of S-Adenosylmethionine Synthetase Catalysis †. Biochemistry 2000, 39: 4443-4454. PMID: 10757994, DOI: 10.1021/bi992876s.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmino Acid SequenceBinding SitesCatalysisComputer SimulationDiphosphatesEscherichia coliFluorescenceHydrolysisIsomerismKineticsLigandsMethionineMethionine AdenosyltransferaseOxygenOxygen IsotopesPhosphatesPolyphosphatesS-AdenosylmethionineSolventsThermodynamicsTitrimetryWaterConceptsFree energy profilesSubstrate bindingLoop movementEnergy profilesFormation of AdoMetS-adenosylmethionineChemical interconversion stepPre-steady-state kineticsS-adenosylmethionine synthetaseProduct releaseP(i) complexEquilibrium binding measurementsEnzyme-catalyzed reactionsAdoMet formationBiological alkylating agentsConcentration of substrateFormation reactionCrystallographic studiesEnzyme turnoverEquilibrium constantsCatalyze formationRate constantsInterconversion stepActive siteBinding energy