2020
A Decoy Library Uncovers U-Box E3 Ubiquitin Ligases That Regulate Flowering Time in Arabidopsis
Feke AM, Hong J, Liu W, Gendron JM. A Decoy Library Uncovers U-Box E3 Ubiquitin Ligases That Regulate Flowering Time in Arabidopsis. Genetics 2020, 215: 699-712. PMID: 32434795, PMCID: PMC7337086, DOI: 10.1534/genetics.120.303199.Peer-Reviewed Original ResearchConceptsE3 ubiquitin ligasesUbiquitin ligasesU-box E3 ubiquitin ligasesReproductive developmentReverse genetic screenMOS4-associated complexDominant-negative strategyGenetic redundancyGenetic screenTransgenic populationsTargeted degradationLigasesUnidentified regulatorDevelopmental transitionsBiological processesGenetic characterizationU-boxBiochemical studiesWidespread importanceRegulatorArabidopsisOrganismsPlantsProteinComplexes
2017
Mapping Protein–Protein Interactions Using Affinity Purification and Mass Spectrometry
Lee CM, Adamchek C, Feke A, Nusinow DA, Gendron JM. Mapping Protein–Protein Interactions Using Affinity Purification and Mass Spectrometry. Methods In Molecular Biology 2017, 1610: 231-249. PMID: 28439867, DOI: 10.1007/978-1-4939-7003-2_15.Peer-Reviewed Original ResearchConceptsAP-MSHigher-order protein complexesAffinity purificationMapping protein-protein interactionsProtein-protein interaction networkProtein-protein interactionsMeaningful complexesProtein interactomeProtein complexesProtein-specific antibodiesMass spectrometry technologyInteraction networksCellular pathwaysTransient interactionsMass spectrometryInteractomeNonspecific interactionsSpectrometry technologyUnbiased methodComplexesPurificationComprehensive knowledgeCrucial stepPlantsProtein