2018
Conventional wastewater treatment and reuse site practices modify bacterial community structure but do not eliminate some opportunistic pathogens in reclaimed water
Kulkarni P, Olson N, Paulson J, Pop M, Maddox C, Claye E, Goldstein R, Sharma M, Gibbs S, Mongodin E, Sapkota A. Conventional wastewater treatment and reuse site practices modify bacterial community structure but do not eliminate some opportunistic pathogens in reclaimed water. The Science Of The Total Environment 2018, 639: 1126-1137. PMID: 29929281, PMCID: PMC8290890, DOI: 10.1016/j.scitotenv.2018.05.178.Peer-Reviewed Original ResearchConceptsWastewater treatment plantsTreatment plantsWastewater treatmentSpray irrigation siteMunicipal wastewater treatment plantU.S. wastewater treatment plantsConventional wastewater treatmentReclaimed waterIrrigated sitesCommunity structureBacterial communitiesFuture treatment technologiesOpen-air storageOn-site treatmentTreatment technologiesAlpha diversity of bacterial communitiesUltraviolet treatmentOperational taxonomic unitsRRNA gene sequencesInfluentBacterial community structureSprayWastewaterPumphouseWater recycling
2017
Temporal Variations in Cigarette Tobacco Bacterial Community Composition and Tobacco-Specific Nitrosamine Content Are Influenced by Brand and Storage Conditions
Chopyk J, Chattopadhyay S, Kulkarni P, Smyth E, Hittle L, Paulson J, Pop M, Buehler S, Clark P, Mongodin E, Sapkota A. Temporal Variations in Cigarette Tobacco Bacterial Community Composition and Tobacco-Specific Nitrosamine Content Are Influenced by Brand and Storage Conditions. Frontiers In Microbiology 2017, 08: 358. PMID: 28326071, PMCID: PMC5339245, DOI: 10.3389/fmicb.2017.00358.Peer-Reviewed Original ResearchOperational taxonomic unitsCore bacterial operational taxonomic unitsStorage conditionsBacterial operational taxonomic unitsV3V4 hypervariable regionBacterial community compositionTaxonomic unitsDay 0Illumina MiSeqRRNA geneCommunity compositionMicrobial ecosystemBacterial communitiesHypervariable regionTobacco-specific nitrosamine contentTime series experimentsBacillus pumilusNitrosamine contentAbundanceRelative humidityStorageIlluminaMiSeqLiquid chromatographyV3V4