2017
Extracellular vesicles carry microRNA‐195 to intrahepatic cholangiocarcinoma and improve survival in a rat model
Li L, Piontek K, Ishida M, Fausther M, Dranoff JA, Fu R, Mezey E, Gould SJ, Fordjour FK, Meltzer SJ, Sirica AE, Selaru FM. Extracellular vesicles carry microRNA‐195 to intrahepatic cholangiocarcinoma and improve survival in a rat model. Hepatology 2017, 65: 501-514. PMID: 27474881, PMCID: PMC5258762, DOI: 10.1002/hep.28735.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBile Duct NeoplasmsCarcinogenesisCell MovementCholangiocarcinomaDisease Models, AnimalDown-RegulationExtracellular VesiclesFibroblastsHumansImmunohistochemistryMaleMicroRNAsRandom AllocationRatsRats, Inbred F344Real-Time Polymerase Chain ReactionSensitivity and SpecificitySurvival RateTransfectionTumor Cells, CulturedTumor MicroenvironmentConceptsExtracellular vesiclesMiR speciesCancer cellsCancer-associated fibroblastsFibroblasts-derived extracellular vesiclesMiR-195Rat modelMicroRNA speciesQuantitative reverse transcription polymerase chain reactionCCA cellsSpeciesCancer developmentCancer fibroblastsHuman cholangiocarcinomaMiR contentReverse transcription-polymerase chain reactionNovel therapeuticsFibroblastsCentral roleSize of cancerVesiclesCellsPolymerase chain reactionMicroRNA-195Cancer microenvironment
2002
The ecto‐nucleoside triphosphate diphosphohydrolase NTPDase2/CD39L1 is expressed in a novel functional compartment within the liver
Dranoff JA, Kruglov EA, Robson SC, Braun N, Zimmermann H, Sévigny J. The ecto‐nucleoside triphosphate diphosphohydrolase NTPDase2/CD39L1 is expressed in a novel functional compartment within the liver. Hepatology 2002, 36: 1135-1144. PMID: 12395323, DOI: 10.1053/jhep.2002.36823.Peer-Reviewed Original ResearchConceptsIntrahepatic bile ductsExtracellular nucleotidesBile ductDiverse biological functionsBlot analysisEcto-nucleoside triphosphate diphosphohydrolasesNTPDase2/CD39L1Portal fibroblastsNorthern blot analysisCellular compartmentsBiological functionsPotential regulatorConfocal immunofluorescenceWestern blot analysisHepatic blood flowBile duct epitheliumReverse transcription-polymerase chain reactionFunctional assaysTriphosphate diphosphohydrolasesImmunoelectron microscopyFunctional compartmentsHepatic central veinNucleotidesNTPDase1NTPDase2Isolation of Primary Rat Liver Fibroblasts
Kruglov EA, Jain D, Dranoff JA. Isolation of Primary Rat Liver Fibroblasts. Journal Of Investigative Medicine 2002, 50: 179. PMID: 12033282, DOI: 10.2310/6650.2002.33431.Peer-Reviewed Original ResearchConceptsLiver fibroblastsProcollagen-1 mRNAReverse transcription-polymerase chain reactionRat liverTranscription-polymerase chain reactionDistinct liver cell populationsSmooth muscle actinSmooth muscle cellsStandard cell culture methodsLiver cell populationsRole of fibroblastsVon Willebrand factorPolymerase chain reactionStellate cellsProcollagen 1Muscle actinCell markersMuscle cellsLiver physiologyAppearance of cellsWillebrand factorFibroblast morphologyChain reactionLiver researchCell populations