2024
On the Ultrastructure of Aberrant Basaloid Cells in Idiopathic Pulmonary Fibrosis.
Schipke J, Panpeng S, Wrede C, Hegermann J, Ruwisch J, Werlein C, Jonigk D, Shin H, Schupp J, Mühlfeld C, Knudsen L. On the Ultrastructure of Aberrant Basaloid Cells in Idiopathic Pulmonary Fibrosis. American Journal Of Respiratory Cell And Molecular Biology 2024, 71: 746-750. PMID: 39601536, DOI: 10.1165/rcmb.2024-0218le.Peer-Reviewed Original ResearchStudying the Pulmonary Endothelium in Health and Disease: An Official American Thoracic Society Workshop Report
Hough R, Alvira C, Bastarache J, Erzurum S, Kuebler W, Schmidt E, Shimoda L, Abman S, Alvarez D, Belvitch P, Bhattacharya J, Birukov K, Chan S, Cornfield D, Dudek S, Garcia J, Harrington E, Hsia C, Islam M, Jonigk D, Kalinichenko V, Kolb T, Lee J, Mammoto A, Mehta D, Rounds S, Schupp J, Shaver C, Suresh K, Tambe D, Ventetuolo C, Yoder M, Stevens T, Damarla M. Studying the Pulmonary Endothelium in Health and Disease: An Official American Thoracic Society Workshop Report. American Journal Of Respiratory Cell And Molecular Biology 2024, 71: 388-406. PMID: 39189891, PMCID: PMC11450313, DOI: 10.1165/rcmb.2024-0330st.Peer-Reviewed Original ResearchVascular diseaseEndothelial cell injuryAmerican Thoracic SocietyEndothelial cell functionPulmonary endotheliumMechanical propertiesLung endotheliumChronic vascular diseaseEndothelial functionPathophysiological mechanismsHealth and DiseaseVascular locationThoracic SocietyCell injuryEndotheliumCell functionTissue compartmentsDiseaseVascular circuitBidirectional signalingTranslation of discoveriesBloodDissecting the immune cell niche in pulmonary sarcoidosis – CXCL10+ monocyte-derived macrophages are potential drivers of TH17.1 inflammation
Ruwisch J, Schupp J, Bartkute B, Artysh N, Kaminski N, Prasse A. Dissecting the immune cell niche in pulmonary sarcoidosis – CXCL10+ monocyte-derived macrophages are potential drivers of TH17.1 inflammation. 2024, oa953. DOI: 10.1183/13993003.congress-2024.oa953.Peer-Reviewed Original ResearchSingle-nuclei RNA-seq reveals aberrant cell populations in restrictive allograft syndrome after lung transplantation
Leiber L, Christian L, Neubert L, Yilmaz H, Kamp J, Plucinski E, Welte T, Falk C, Kaminski N, Jonigk* D, Gottlieb* J, Schupp* J. Single-nuclei RNA-seq reveals aberrant cell populations in restrictive allograft syndrome after lung transplantation. 2024, oa985. DOI: 10.1183/13993003.congress-2024.oa985.Peer-Reviewed Original ResearchNovel insight into alveolar capillary dysplasia from multi-omics and 3D imaging
Park D, Kamp J, Neubert L, Freitag H, Schupp J, Braubach P, Christoph W, Florian L, Salditt T, Reichmann J, Welte T, Ruhparwar A, Ius F, Schwerk N, Bergmann A, Von Hardenberg S, Griese M, Rapp C, Olsson K, Fuge J, Hoeper M, Jonigk D, Knudsen L, Kuehnel M. Novel insight into alveolar capillary dysplasia from multi-omics and 3D imaging. 2024, oa1900. DOI: 10.1183/13993003.congress-2024.oa1900.Peer-Reviewed Original Research129Xe magnetic resonance imaging for monitoring lung function and microstructure in patients on treatment for progressive fibrosing interstitial lung disease
Kern A, Voskrebenzev A, Klimeš F, Pink I, Schupp J, Seeliger B, Konietzke M, Peter D, Risse F, Welte T, Wacker F, Hohlfeld J, Shin H, Prasse A, Vogel-Claussen J. 129Xe magnetic resonance imaging for monitoring lung function and microstructure in patients on treatment for progressive fibrosing interstitial lung disease. 2024, oa5465. DOI: 10.1183/13993003.congress-2024.oa5465.Peer-Reviewed Original ResearchSingle-Cell Profiling Reveals Immune Aberrations in Progressive Idiopathic Pulmonary Fibrosis.
Unterman A, Zhao A, Neumark N, Schupp J, Ahangari F, Cosme C, Sharma P, Flint J, Stein Y, Ryu C, Ishikawa G, Sumida T, Gomez J, Herazo-Maya J, Dela Cruz C, Herzog E, Kaminski N. Single-Cell Profiling Reveals Immune Aberrations in Progressive Idiopathic Pulmonary Fibrosis. American Journal Of Respiratory And Critical Care Medicine 2024, 210: 484-496. PMID: 38717443, PMCID: PMC11351796, DOI: 10.1164/rccm.202306-0979oc.Peer-Reviewed Original ResearchStable idiopathic pulmonary fibrosisIdiopathic pulmonary fibrosisPeripheral blood mononuclear cellsProgressive idiopathic pulmonary fibrosisPeripheral immune systemT cellsPulmonary fibrosisCohort of IPF patientsAssociated with decreased survivalIdiopathic pulmonary fibrosis patientsPeripheral blood mononuclear cell samplesPeripheral blood cell populationsImmune systemFraction of TregsRegulatory T cellsBlood mononuclear cellsBlood cell populationsFlow cytometry analysisImmune aberrationsIPF patientsTregsMononuclear cellsSingle-cell RNA sequencingLung homogenatesMonocyte chemoattractantSingle-Cell Analysis Reveals Novel Immune Perturbations in Fibrotic Hypersensitivity Pneumonitis.
Zhao A, Unterman A, Abu Hussein N, Sharma P, Nikola F, Flint J, Yan X, Adams T, Justet A, Sumida T, Zhao J, Schupp J, Raredon M, Ahangari F, Deluliis G, Zhang Y, Buendia-Roldan I, Adegunsoye A, Sperling A, Prasse A, Ryu C, Herzog E, Selman M, Pardo A, Kaminski N. Single-Cell Analysis Reveals Novel Immune Perturbations in Fibrotic Hypersensitivity Pneumonitis. American Journal Of Respiratory And Critical Care Medicine 2024, 210: 1252-1266. PMID: 38924775, PMCID: PMC11568434, DOI: 10.1164/rccm.202401-0078oc.Peer-Reviewed Original ResearchFibrotic hypersensitivity pneumonitisIdiopathic pulmonary fibrosisPeripheral blood mononuclear cellsBronchoalveolar lavage cellsBlood mononuclear cellsClassical monocytesHypersensitivity pneumonitisPulmonary fibrosisT cellsImmune perturbationsLavage cellsMononuclear cellsCD8+ T cellsCytotoxic T cellsInterstitial lung diseaseHypersensitivity pneumonitis patientsCytotoxic CD4Immune aberrationsPneumonic patientsPneumonitisLung diseaseHealthy controlsImmune mechanismsPatient cellsSingle-cell transcriptomicsCellular Mechanisms Underlying the Pathogenetic Role of the Primary Cilium in Pulmonary Arterial Hypertension
Melnikov A, Solymosi P, Pennitz P, Schupp J, Nouailles G, Perret P, Gallo K, Hegemann N, Nambiar Veetil N, Klepetko W, Sinn K, Erfinanda L, Simmons S, Kucherenko M, Knosalla C, Preissner R, Kwapiszewska G, Witzenrath M, Kuebler W. Cellular Mechanisms Underlying the Pathogenetic Role of the Primary Cilium in Pulmonary Arterial Hypertension. 2024, a6790-a6790. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a6790.Peer-Reviewed Original ResearchsnRNAseq of IPF Stromal Cells Reveals Dysfunctional Gene Co-expression Profile
Sharma P, Taylor S A, Anderson S, Nekola F, Giuseppe D, Yan X, Schupp J, Balayev A, Justet A, Wuyts W, Vanaudenaerde B, Kaminski N. snRNAseq of IPF Stromal Cells Reveals Dysfunctional Gene Co-expression Profile. 2024, a4903-a4903. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a4903.Peer-Reviewed Original ResearchIPF Aberrant Basaloid Cells Have Chromatin Accessibility Features Distinct From Other Lung Epithelial Cells
Adams T, Schupp J, Balayev A, Justet A, Sharma P, Anderson S, Nekola F, Deiuliis G, Yan X, Wuyts W, Vanaudenaerde B, Kaminski N. IPF Aberrant Basaloid Cells Have Chromatin Accessibility Features Distinct From Other Lung Epithelial Cells. 2024, a4898-a4898. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a4898.Peer-Reviewed Original ResearchCXCL10+ Monocyte-derived Macrophages Are Potential Drivers of TH17.1 Inflammation in Pulmonary Sarcoidosis
Ruwisch J, Schupp J, Bartkute B, Artysh N, Kaminski N, Prasse A. CXCL10+ Monocyte-derived Macrophages Are Potential Drivers of TH17.1 Inflammation in Pulmonary Sarcoidosis. 2024, a2510-a2510. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a2510.Peer-Reviewed Original ResearchThree-dimensional Analysis of the Microvasculature in Alveolar Capillary Dysplasia
Kamp J, Neubert L, Schupp J, Braubach P, Wrede C, Laenger F, Salditt T, Reichmann J, Welte T, Ruhparwar A, Ius F, Schwerk N, Bergmann A, von Hardenberg S, Griese M, Rapp C, Olsson K, Fuge J, Park D, Hoeper M, Jonigk D, Knudsen L, Kuehnel M. Three-dimensional Analysis of the Microvasculature in Alveolar Capillary Dysplasia. 2024, a5157-a5157. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a5157.Peer-Reviewed Original ResearchEx Vivo Testing of Antifibrotic Drugs in a Long-term Culture of Precision-cut Lung Slices (PCLS)
Artysh N, Jäger B, Schupp J, Sewald K, Ruwisch J, Schenk A, Zeug A, Prasse A. Ex Vivo Testing of Antifibrotic Drugs in a Long-term Culture of Precision-cut Lung Slices (PCLS). 2024, a2540-a2540. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a2540.Peer-Reviewed Original ResearchThe nature of chronic rejection after lung transplantation: a murine orthotopic lung transplant study
Heigl T, Kaes J, Aelbrecht C, Serré J, Yamada Y, Geudens V, Van Herck A, Vanstapel A, Sacreas A, Ordies S, Frick A, Gimenez B, Van Slambrouck J, Beeckmans H, Oztürk N, Orlitova M, Vaneylen A, Claes S, Schols D, Velde G, Schupp J, Kaminski N, Boesch M, Korf H, van der Merwe S, Dupont L, Vanoirbeek J, Godinas L, Van Raemdonck D, Janssens W, Gayan-Ramirez G, Ceulemans L, McDonough J, Verbeken E, Vos R, Vanaudenaerde B. The nature of chronic rejection after lung transplantation: a murine orthotopic lung transplant study. Frontiers In Immunology 2024, 15: 1369536. PMID: 38736881, PMCID: PMC11084670, DOI: 10.3389/fimmu.2024.1369536.Peer-Reviewed Original ResearchConceptsChronic lung allograft dysfunctionChronic rejectionLung transplantationSingle cell RNASingle cell RNA profilingOrthotopic left lung transplantationComplications post-transplantationLung allograft dysfunctionMouse lung transplantationLeft lung transplantationLung transplantation studiesAllograft dysfunctionPleural infiltrationPost-transplantationBronchiolitis obliteransInnate inflammationChronic complicationsPrimary onsetEndothelial destructionTransplantationDay 7Immunological perspectiveArterial responseTransplantation studiesLungComparison of organ involvement clusters in Black and White American sarcoidosis patients from a prospectively collected patient registry
Harper L, Tauquir A, Huang S, Wang X, Schupp J, Baughman R, Culver D. Comparison of organ involvement clusters in Black and White American sarcoidosis patients from a prospectively collected patient registry. Respiratory Medicine 2024, 226: 107605. PMID: 38537782, DOI: 10.1016/j.rmed.2024.107605.Peer-Reviewed Original ResearchFibrotic cocktail treated human precision lung slices replicate the cellular diversity of the IPF lung
Justet A, Pineda H, Adams T, Balayev A, Mitash N, Ishizuka M, Kim H, Khoury J, Cala-García J, Flint J, Schupp J, Ahangari F, Yan X, Rosas I, Kaminski N, Königshoff M. Fibrotic cocktail treated human precision lung slices replicate the cellular diversity of the IPF lung. Revue Des Maladies Respiratoires 2024, 41: 218. DOI: 10.1016/j.rmr.2024.01.074.Peer-Reviewed Original ResearchCellular repertoireCell typesSingle cell platformsSequence readsCDNA libraryIllumina platformHuman genomeNucleus transcriptomicsCellular diversityIPF lungsPulmonary fibrosisEMT markersAirway epithelial cellsBasaloid cellsCellular populationsEpithelial cellsFibrotic fibroblastsCell platformLung slicesLung cell populationsHuman precision-cut lung slicesCell populationsSenescence markersCellsBasal markersCXCL10+ Monozyten abgeleitete Makrophagen sind potentielle Treiber der TH17.1 Entzündung bei pulmonaler Sarkoidose
Ruwisch J, Artysh N, Bartkute B, Schupp J, Prasse A. CXCL10+ Monozyten abgeleitete Makrophagen sind potentielle Treiber der TH17.1 Entzündung bei pulmonaler Sarkoidose. Pneumologie 2024, 78: s119-s119. DOI: 10.1055/s-0044-1778996.Peer-Reviewed Original ResearchTherapie der Sarkoidose. Ein Positionspapier der Deutschen Gesellschaft für Pneumologie und Beatmungsmedizin (DGP)
Skowasch D, Bonella F, Buschulte K, Kneidinger N, Korsten P, Kreuter M, Müller-Quernheim J, Pfeifer M, Prasse A, Quadder B, Sander O, Schupp J, Sitter H, Stachetzki B, Grohé C. Therapie der Sarkoidose. Ein Positionspapier der Deutschen Gesellschaft für Pneumologie und Beatmungsmedizin (DGP). Pneumologie 2024, 78: 151-166. PMID: 38408486, DOI: 10.1055/a-2259-1046.Peer-Reviewed Original ResearchSingle-cell transcriptomic analysis of human pleura reveals stromal heterogeneity and informs in vitro models of mesothelioma
Obacz J, Valer J, Nibhani R, Adams T, Schupp J, Veale N, Lewis-Wade A, Flint J, Hogan J, Aresu G, Coonar A, Peryt A, Biffi G, Kaminski N, Francies H, Rassl D, Garnett M, Rintoul R, Marciniak S. Single-cell transcriptomic analysis of human pleura reveals stromal heterogeneity and informs in vitro models of mesothelioma. European Respiratory Journal 2024, 63: 2300143. PMID: 38212075, PMCID: PMC10809128, DOI: 10.1183/13993003.00143-2023.Peer-Reviewed Original ResearchConceptsSingle-cell transcriptome atlasSingle-cell levelSingle-cell transcriptome analysisTranscriptome dataTranscriptomic atlasTranscriptomic characterisationMesothelial cellsCell atlasDevelopment of targeted therapiesMalignant mesothelial cellsModel of mesotheliomaUniversal fibroblastsIn vitro model