2023
2368. SARS-CoV-2 mRNA vaccination induces B cell immunity in the tonsils and adenoids of children
Xu Q, Mudd P, Behzadpour H, Bellusci L, Grubbs G, Pourhashemi S, Tang J, Liu C, Newman D, Shi L, Milanez-Almeida P, Kardava L, Tsang J, Moir S, Khurana S, Schwartzberg P, Manthiram K. 2368. SARS-CoV-2 mRNA vaccination induces B cell immunity in the tonsils and adenoids of children. Open Forum Infectious Diseases 2023, 10: ofad500.1989. PMCID: PMC10677082, DOI: 10.1093/ofid/ofad500.1989.Peer-Reviewed Original ResearchSARS-CoV-2-specific B cellsMemory B cellsB cellsAdenoids of childrenMRNA vaccinesVaccinated subjectsPeripheral bloodInfected subjectsUpper respiratory tract lymphoid tissueSARS-CoV-2IgA+ memory B cellsSARS-CoV-2 mRNA vaccinesGerminal center B cellsRobust adaptive immune responsesPost-vaccinationCOVID-19 mRNA vaccinesB-cell phenotypeB cell immunityAdaptive immune responsesImmunity to SARS-CoV-2SARS-CoV-2 infectionPost-infectionLymphoid tissueAdenoidsMucosal IgA
2022
Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children
Xu Q, Milanez-Almeida P, Martins A, Radtke A, Hoehn K, Oguz C, Chen J, Liu C, Tang J, Grubbs G, Stein S, Ramelli S, Kabat J, Behzadpour H, Karkanitsa M, Spathies J, Kalish H, Kardava L, Kirby M, Cheung F, Preite S, Duncker P, Kitakule M, Romero N, Preciado D, Gitman L, Koroleva G, Smith G, Shaffer A, McBain I, McGuire P, Pittaluga S, Germain R, Apps R, Schwartz D, Sadtler K, Moir S, Chertow D, Kleinstein S, Khurana S, Tsang J, Mudd P, Schwartzberg P, Manthiram K. Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children. Nature Immunology 2022, 24: 186-199. PMID: 36536106, PMCID: PMC10777159, DOI: 10.1038/s41590-022-01367-z.Peer-Reviewed Original ResearchConceptsT cell receptorImmune responseGerminal centersPrevious SARS-CoV-2 infectionSARS-CoV-2 infectionB-cell receptor sequencingTissue-specific immunityCell receptor sequencingAdaptive immune responsesUpper respiratory tractMemory B cellsT cell clonotypesSite of infectionSARS-CoV-2Pharyngeal lymphoid tissuePeripheral bloodLymphocyte populationsLymphoid tissueRespiratory tractCell clonotypesAdaptive immunityB cellsCDR3 sequencesAdenoidsCell receptor
2019
Overexpression of T-bet in HIV infection is associated with accumulation of B cells outside germinal centers and poor affinity maturation
Austin JW, Buckner CM, Kardava L, Wang W, Zhang X, Melson VA, Swanson RG, Martins AJ, Zhou JQ, Hoehn KB, Fisk JN, Dimopoulos Y, Chassiakos A, O'Dell S, Smelkinson MG, Seamon CA, Kwan RW, Sneller MC, Pittaluga S, Doria-Rose NA, McDermott A, Li Y, Chun TW, Kleinstein SH, Tsang JS, Petrovas C, Moir S. Overexpression of T-bet in HIV infection is associated with accumulation of B cells outside germinal centers and poor affinity maturation. Science Translational Medicine 2019, 11 PMID: 31776286, PMCID: PMC7479651, DOI: 10.1126/scitranslmed.aax0904.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntibodies, NeutralizingAntibody AffinityAntigens, CD19B-LymphocytesCytokinesFemaleGerminal CenterHIV InfectionsHumansImmunologic MemoryLymph NodesMaleMiddle AgedMutation RatePhenotypeReceptors, Antigen, B-CellT-Box Domain ProteinsT-Lymphocytes, Helper-InducerTranscriptomeYoung AdultConceptsHIV-specific B cellsT-betGC B cellsGerminal centersB cellsLymph nodesPoor affinity maturationChronic immune activationMemory B cell compartmentAntibody-mediated immunityChronic infectious diseaseOptimal antibody responseB cell compartmentChronic human infectionsB cell receptorHIV viremiaImmunologic outcomesHIV infectionViremic individualsChronic viremiaImmune activationPeripheral bloodProtective antibodiesAntibody responseCD19
2014
Abnormal B cell memory subsets dominate HIV-specific responses in infected individuals
Kardava L, Moir S, Shah N, Wang W, Wilson R, Buckner CM, Santich BH, Kim LJ, Spurlin EE, Nelson AK, Wheatley AK, Harvey CJ, McDermott AB, Wucherpfennig KW, Chun TW, Tsang JS, Li Y, Fauci AS. Abnormal B cell memory subsets dominate HIV-specific responses in infected individuals. Journal Of Clinical Investigation 2014, 124: 3252-3262. PMID: 24892810, PMCID: PMC4071400, DOI: 10.1172/jci74351.Peer-Reviewed Original ResearchConceptsHIV-specific responsesMemory B cellsB cellsInfected individualsMemory subsetsUninfected individualsVirus-specific B cell responsesMemory B cell subsetsPeripheral blood B cellsCertain infected individualsHIV-specific antibodiesB cell abnormalitiesAbnormal B cellsB-cell dysfunctionB cell responsesBlood B cellsB cell subsetsStage of infectionPlasma viremiaAntiretroviral therapyHIV antibodiesHIV diseaseHIV infectionImmune activationCell subsets