2018
Label‐free high‐speed wide‐field imaging of single microtubules using interference reflection microscopy
MAHAMDEH M, SIMMERT S, LUCHNIAK A, SCHÄFFER E, HOWARD J. Label‐free high‐speed wide‐field imaging of single microtubules using interference reflection microscopy. Journal Of Microscopy 2018, 272: 60-66. PMID: 30044498, PMCID: PMC6486835, DOI: 10.1111/jmi.12744.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleFluorescenceLightMicroscopy, FluorescenceMicroscopy, InterferenceMicrotubulesConceptsDifferential interference contrastReflection microscopyInterference contrastCostly optical componentsWide-field imagingLabel-free imagingHollow cylindrical beamsDark-field condenserInterference contrast imagesDifferential interference contrast (DIC) imagesEfficient fluorescent labelingOptical componentsInterference reflection microscopyOptical misalignmentMicroscope modificationsProtein building blocksNomarski prismOptical qualityHigh-speed imagingNumber of drawbacksFree imagingLight interferenceCylindrical beamsContrast imagesHigh contrast
2008
XMAP215 Is a Processive Microtubule Polymerase
Brouhard GJ, Stear JH, Noetzel TL, Al-Bassam J, Kinoshita K, Harrison SC, Howard J, Hyman AA. XMAP215 Is a Processive Microtubule Polymerase. Cell 2008, 132: 79-88. PMID: 18191222, PMCID: PMC2311386, DOI: 10.1016/j.cell.2007.11.043.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesBiological AssayCatalytic DomainCell DifferentiationCell EnlargementCell LineCytoskeletonDiffusionDimerizationFetal ProteinsForminsGreen Fluorescent ProteinsMicrofilament ProteinsMicroscopy, FluorescenceMicrotubule-Associated ProteinsMicrotubulesNuclear ProteinsPolymersProtein BindingProtein Structure, TertiaryProtein TransportSpodopteraSus scrofaTubulinXenopus laevisXenopus ProteinsConceptsFamily of proteinsSingle-molecule assaysActin polymerasesMicrotubule cytoskeletonProcessive polymeraseTubulin subunit additionDynamic microtubulesMicrotubule polymeraseMicrotubule growthCytoskeletal polymersXMAP215Plus endsMicrotubule latticePolymeraseSubunit additionCell proliferationFree tubulinTubulin dimersMicrotubulesCommon mechanismFast growthTip trackingGrowthCytoskeletonRapid assembly