2002
Functional analysis of basic transcription element (BTE)-binding protein (BTEB) 3 and BTEB4, a novel Sp1-like protein, reveals a subfamily of transcriptional repressors for the BTE site of the cytochrome P4501A1 gene promoter
KACZYNSKI J, CONLEY A, ZAPICO M, DELGADO S, ZHANG J, URRUTIA R. Functional analysis of basic transcription element (BTE)-binding protein (BTEB) 3 and BTEB4, a novel Sp1-like protein, reveals a subfamily of transcriptional repressors for the BTE site of the cytochrome P4501A1 gene promoter. Biochemical Journal 2002, 366: 873-882. PMID: 12036432, PMCID: PMC1222816, DOI: 10.1042/bj20020388.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBlotting, NorthernBlotting, WesternCarcinogensCell Cycle ProteinsCell LineCHO CellsChromatinCricetinaeCytochrome P-450 CYP1A1DNA, ComplementaryDNA-Binding ProteinsDose-Response Relationship, DrugGlutathione TransferaseHumansKruppel-Like Transcription FactorsMolecular Sequence DataPlasmidsPromoter Regions, GeneticProtein BindingProtein Structure, TertiaryRecombinant Fusion ProteinsRepressor ProteinsSequence Homology, Amino AcidSp1 Transcription FactorTrans-ActivatorsTranscription FactorsTranscription, GeneticZinc FingersConceptsGene promoterCYP1A1 gene promoterProtein familyC-terminal zinc-finger motifC-terminal zinc-finger domainBind GC-rich sequencesControl of gene expressionCo-repressor mSin3ASp1-like proteinsZinc-finger motifGC-rich sequencesFamily of proteinsN-terminal regionRepress transcriptionTranscriptional repressionCo-repressorTranscriptional repressorCellular machineryExpressed memberTranscriptional elementsTranscription factorsFunctional characterizationBTEB3Gene expressionTranscriptionSignaling disrupts mSin3A binding to the Mad1‐like Sin3‐interacting domain of TIEG2, an Sp1‐like repressor
Ellenrieder V, Zhang J, Kaczynski J, Urrutia R. Signaling disrupts mSin3A binding to the Mad1‐like Sin3‐interacting domain of TIEG2, an Sp1‐like repressor. The EMBO Journal 2002, 21: 2451-2460. PMID: 12006497, PMCID: PMC126002, DOI: 10.1093/emboj/21.10.2451.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SequenceAnimalsBinding SitesCell Cycle ProteinsCHO CellsConsensus SequenceCricetinaeGenes, ReporterKruppel-Like Transcription FactorsMicePhosphorylationProtein BindingRecombinant ProteinsRepressor ProteinsSignal TransductionSin3 Histone Deacetylase and Corepressor ComplexSp1 Transcription FactorTranscription FactorsTransfectionZinc FingersConceptsSin3 interaction domainTranscriptional repressionAnti-proliferative functionMad proteinsRepressor proteinRepression activitySerine/threonine sitesTranscription factorsConstitutive mannerSignaling pathwayRepressionGrowth suppressionFunctional impactTIEG2ProteinRepressorSerine/threonineTIEGTranscriptionPhosphorylationDomainSignalPathwayInteractionBinding
2001
A Conserved α-Helical Motif Mediates the Interaction of Sp1-Like Transcriptional Repressors with the Corepressor mSin3A
Zhang J, Moncrieffe M, Kaczynski J, Ellenrieder V, Prendergast F, Urrutia R. A Conserved α-Helical Motif Mediates the Interaction of Sp1-Like Transcriptional Repressors with the Corepressor mSin3A. Molecular And Cellular Biology 2001, 21: 5041-5049. PMID: 11438660, PMCID: PMC87230, DOI: 10.1128/mcb.21.15.5041-5049.2001.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceAnimalsApoptosis Regulatory ProteinsBlotting, WesternCell Cycle ProteinsCell DivisionCHO CellsCircular DichroismCricetinaeGenetic VectorsGlutathione TransferaseLuciferasesMolecular Sequence DataMutationPeptide BiosynthesisPlasmidsPrecipitin TestsProtein BindingProtein BiosynthesisProtein Structure, TertiaryRecombinant Fusion ProteinsRepressor ProteinsSequence Homology, Amino AcidSin3 Histone Deacetylase and Corepressor ComplexSp1 Transcription FactorTranscription, GeneticTransforming Growth Factor betaZinc FingersConceptsSp1-like proteinsRepress transcriptionDeacetylase complexRepression motifTranscriptional repressionSin3 histone deacetylase complexBind GC-rich sequencesMechanism of transcriptional repressionSp1-like transcription factorsMSin3A-histone deacetylase complexGC-rich sequencesMammalian cell homeostasisCorepressor mSin3ARepression domainTranscriptional repressorA-helicesMSin3ATranscription factorsTIEG2Antiproliferative functionCell homeostasisMotifTranscriptionRepressionProteinThe Sp1-like Protein BTEB3 Inhibits Transcription via the Basic Transcription Element Box by Interacting with mSin3A and HDAC-1 Co-repressors and Competing with Sp1*
Kaczynski J, Zhang J, Ellenrieder V, Conley A, Duenes T, Kester H, van der Burg B, Urrutia R. The Sp1-like Protein BTEB3 Inhibits Transcription via the Basic Transcription Element Box by Interacting with mSin3A and HDAC-1 Co-repressors and Competing with Sp1*. Journal Of Biological Chemistry 2001, 276: 36749-36756. PMID: 11477107, DOI: 10.1074/jbc.m105831200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAnimalsBinding, CompetitiveBlotting, WesternCell Cycle ProteinsCHO CellsCricetinaeGenes, ReporterGlutathione TransferaseHistone Deacetylase 1Histone DeacetylasesKineticsKruppel-Like Transcription FactorsMolecular Sequence DataPlasmidsPrecipitin TestsPromoter Regions, GeneticProtein BindingProtein Structure, TertiaryRepressor ProteinsSin3 Histone Deacetylase and Corepressor ComplexSp1 Transcription FactorTrans-ActivatorsTranscription, GeneticZinc FingersConceptsCo-repressorN-terminusHDAC-1Bind GC-rich sequencesC-terminal zinc finger motifsGC-rich DNA elementsCo-repressor mSin3ASp1-like proteinsZinc finger motifsDNA-binding domainGC-rich sequencesSp1-like activityGel shift assaysMammalian cell homeostasisMechanism of repressionChinese hamster ovary cellsFinger motifDNA elementsSp1 familyRepressor domainRepressor proteinBTEB3GAL4 assayTranscriptional repressionTranscriptional repressorMolecular mechanisms of transcriptional repression shared by the growth regulatory Sp1-like proteins and the tumor suppressor Mad1
Zhang J, Moncrieffe M, Kaczynski J, Prendergast F, Urrutia R. Molecular mechanisms of transcriptional repression shared by the growth regulatory Sp1-like proteins and the tumor suppressor Mad1. Gastroenterology 2001, 120: a139. DOI: 10.1016/s0016-5085(08)80685-3.Peer-Reviewed Original ResearchMolecular mechanisms of transcriptional repression shared by the growth regulatory Sp1-like proteins and the tumor suppressor Mad1
ZHANG J, MONCRIEFFE M, KACZYNSKI J, PRENDERGAST F, URRUTIA R. Molecular mechanisms of transcriptional repression shared by the growth regulatory Sp1-like proteins and the tumor suppressor Mad1. Gastroenterology 2001, 120: a139-a139. DOI: 10.1016/s0016-5085(01)80685-5.Peer-Reviewed Original Research