2008
Flexibility in the site of exon junction complex deposition revealed by functional group and RNA secondary structure alterations in the splicing substrate
Mishler DM, Christ AB, Steitz JA. Flexibility in the site of exon junction complex deposition revealed by functional group and RNA secondary structure alterations in the splicing substrate. RNA 2008, 14: 2657-2670. PMID: 18952819, PMCID: PMC2590960, DOI: 10.1261/rna.1312808.Peer-Reviewed Original ResearchConceptsExon junction complexRNA secondary structureEJC depositionSplicing substrateMammalian nonsense-mediated mRNA decayNonsense-mediated mRNA decaySecondary structureStretches of DNATranslational regulationMRNA decayCoimmunoprecipitation assaysJunction complexSecondary structure alterationsDNA nucleotidesStructure alterationsH protectionUpstream shiftToeprintingExonsSitesNucleotidesDNACrystal structureDeposition sitesMRNA
2001
Internal Modification of U2 Small Nuclear (Snrna) Occurs in Nucleoli of Xenopus Oocytes
Yu Y, Shu M, Narayanan A, Terns R, Terns M, Steitz J. Internal Modification of U2 Small Nuclear (Snrna) Occurs in Nucleoli of Xenopus Oocytes. Journal Of Cell Biology 2001, 152: 1279-1288. PMID: 11257127, PMCID: PMC2199211, DOI: 10.1083/jcb.152.6.1279.Peer-Reviewed Original ResearchConceptsNucleolar localizationCajal bodiesU2 RNAInternal modificationSmall nuclearSm binding siteNucleolar localization signalSmall nucleolar RNAsXenopus oocytesCytoplasm of oocytesU2 small nuclearGuanosine capLocalization signalNucleolar RNAsRNAs showSubcellular sitesIntranuclear localizationIsolated nucleiRNABinding sitesNucleoliOocytesNucleotidesCytoplasmU2
1999
Guided tours: from precursor snoRNA to functional snoRNP
Weinstein L, Steitz J. Guided tours: from precursor snoRNA to functional snoRNP. Current Opinion In Cell Biology 1999, 11: 378-384. PMID: 10395551, DOI: 10.1016/s0955-0674(99)80053-2.Peer-Reviewed Original Research
1997
A new method for detecting sites of 2'-O-methylation in RNA molecules.
Yu YT, Shu MD, Steitz JA. A new method for detecting sites of 2'-O-methylation in RNA molecules. RNA 1997, 3: 324-31. PMID: 9056769, PMCID: PMC1369484.Peer-Reviewed Original ResearchConceptsRNA moleculesEukaryotic ribosomal RNALong RNA moleculesSpecific rRNARibosomal RNAModification sitesRRNAMethylationCell nucleoliChimeric oligonucleotideSnoRNAsRNase H cleavagePrecursor moleculesRRNA transportSitesMoleculesRNANucleotidesCytoplasmNucleoliResiduesCleavageOligonucleotideH cleavageNucleus
1993
The Cellular RNA-Binding Protein EAP Recognizes a Conserved Stem-Loop in the Epstein-Barr Virus Small RNA EBER 1
Toczyski D, Steitz J. The Cellular RNA-Binding Protein EAP Recognizes a Conserved Stem-Loop in the Epstein-Barr Virus Small RNA EBER 1. Molecular And Cellular Biology 1993, 13: 703-710. DOI: 10.1128/mcb.13.1.703-710.1993.Peer-Reviewed Original ResearchSmall RNAsCellular RNA-binding proteinsConserved stem loopRNA-binding proteinDetailed mutational analysisStem-loop structureSequence-specific mannerRNase protection experimentsDouble-stranded regionsDeletion mutantsEBER-1Mutational analysisStem loopFusion proteinProtection experimentsViral proteinsRNAProteinEpstein-Barr virus small RNAsEpstein-Barr virusBindingMutantsHerpesvirus papioNucleotidesHairpinThe cellular RNA-binding protein EAP recognizes a conserved stem-loop in the Epstein-Barr virus small RNA EBER 1.
Toczyski DP, Steitz JA. The cellular RNA-binding protein EAP recognizes a conserved stem-loop in the Epstein-Barr virus small RNA EBER 1. Molecular And Cellular Biology 1993, 13: 703-710. PMID: 8380232, PMCID: PMC358948, DOI: 10.1128/mcb.13.1.703.Peer-Reviewed Original ResearchConceptsCellular RNA-binding proteinsRNA-binding proteinDetailed mutational analysisStem-loop structureSequence-specific mannerRNase protection experimentsEpstein‐Barr virus small RNA EBER‐1Double-stranded regionsSmall RNAsDeletion mutantsEBER-1Mutational analysisFusion proteinProtection experimentsViral proteinsProteinRNAEpstein-Barr virusBindingMutantsHerpesvirus papioNucleotidesHairpinAntibodiesCells
1990
Spliced leader RNA sequences can substitute for the essential 5′ end of U1 RNA during splicing in a mammalian in vitro system
Bruzik J, Steitz J. Spliced leader RNA sequences can substitute for the essential 5′ end of U1 RNA during splicing in a mammalian in vitro system. Cell 1990, 62: 889-899. PMID: 2168293, DOI: 10.1016/0092-8674(90)90264-f.Peer-Reviewed Original Research
1989
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
Tyc K, Steitz JA. U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus. The EMBO Journal 1989, 8: 3113-3119. PMID: 2531075, PMCID: PMC401391, DOI: 10.1002/j.1460-2075.1989.tb08463.x.Peer-Reviewed Original Research
1983
A mammalian tRNA His-Containing antigen is recognized by the polymyosits-specific antibody antijo-1
Rosa M, Hendrick J, Lerner M, Steitz J, Reichlin M. A mammalian tRNA His-Containing antigen is recognized by the polymyosits-specific antibody antijo-1. Nucleic Acids Research 1983, 11: 853-870. PMID: 6188108, PMCID: PMC325757, DOI: 10.1093/nar/11.3.853.Peer-Reviewed Original ResearchConceptsMammalian cell cytoplasmWobble positionMammalian tRNAHuman HeLaProteinase K treatmentRNA sequencesBase queuineTerminal nucleotidesCell extractsTRNACell cytoplasmAntigenic proteinsImmunofluorescence studiesPhenol extractionK treatmentSephacryl SMethylated guaninesAntigenic formsTRNAHisAntigenic complexQueuineAnticodonUnusual featuresColumn chromatographyNucleotides
1977
RNA·RNA and Protein·RNA Interactions During the Initiation of Protein Synthesis
STEITZ J, SPRAGUE K, STEEGE D, YUAN R, LAUGHREA M, MOORE P, WAHBA A. RNA·RNA and Protein·RNA Interactions During the Initiation of Protein Synthesis. 1977, 491-508. DOI: 10.1016/b978-0-12-722560-9.50032-9.Peer-Reviewed Original Research
1974
Specific recognition of the isolated R17 replicase initiator region by R17 coat protein
STEITZ J. Specific recognition of the isolated R17 replicase initiator region by R17 coat protein. Nature 1974, 248: 223-225. PMID: 4819415, DOI: 10.1038/248223a0.Peer-Reviewed Original Research