2020
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy.
Jacob L, Brito J, Thomas JL. Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy. Journal Of Visualized Experiments 2020 PMID: 32510513, DOI: 10.3791/61099.Peer-Reviewed Original ResearchMeSH KeywordsCentral Nervous SystemHumansImaging, Three-DimensionalLightLymphatic VesselsMicroscopy, FluorescenceNeovascularization, PhysiologicSpineConceptsLight sheet fluorescence microscopySheet fluorescence microscopyLymphatic vasculatureFluorescence microscopyCentral nervous systemLymphatic vesselsBiologyLymphatic systemLymphatic networkJoint biologyWhole-mount preparationsUnprecedented opportunityImmune surveillanceNervous systemImmune cellsTissueMount preparationsVertebral columnCNS tissueCellsMagnaMacromolecules
2019
Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis
Genet G, Boyé K, Mathivet T, Ola R, Zhang F, Dubrac A, Li J, Genet N, Henrique Geraldo L, Benedetti L, Künzel S, Pibouin-Fragner L, Thomas JL, Eichmann A. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis. Nature Communications 2019, 10: 2350. PMID: 31138815, PMCID: PMC6538628, DOI: 10.1038/s41467-019-10359-x.Peer-Reviewed Original ResearchMeSH KeywordsAcyltransferasesAnimalsCell MovementCell PolarityCell ProliferationCell SurvivalEndocytosisEndothelial CellsIntercellular Signaling Peptides and ProteinsMAP Kinase Signaling SystemMiceMice, KnockoutNeovascularization, PhysiologicNerve Tissue ProteinsP21-Activated KinasesReceptors, ImmunologicRetinal VesselsVascular Endothelial Growth Factor Receptor-2ConceptsEndophilin A2Endothelial cell migrationSprouting angiogenesisCell migrationFront-rear polarityBAR domain proteinsFront-rear polarizationClathrin-independent internalizationSpecific endocytic pathwaysVEGFR2 endocytosisEndocytic pathwayAngiogenesis defectsEffector PAKTip cellsSlit-RoboActivation of VEGFR2Downstream activationVEGFR2 internalizationCell behaviorPathological angiogenesisCritical mediatorEndocytosisPathological conditions
2015
Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization
Dubrac A, Genet G, Ola R, Zhang F, Pibouin-Fragner L, Han J, Zhang J, Thomas JL, Chedotal A, Schwartz MA, Eichmann A. Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization. Circulation 2015, 133: 409-421. PMID: 26659946, PMCID: PMC4729599, DOI: 10.1161/circulationaha.115.017537.Peer-Reviewed Original ResearchConceptsFront-rear polaritySprouting angiogenesisSignal integration mechanismImportant drug targetsNck adaptorsCytoskeletal dynamicsEndothelial cell migrationEmbryonic developmentAngiogenesis defectsPAK2 activationVessel sproutsNumber of diseasesBlood vessel growthDrug targetsCell migrationPostnatal retinaAngiogenic growthNckNck1AdaptorVessel growthKey processesEndothelial cellsPathological ocular neovascularizationInhibits neovascularization
2011
Vascular endothelial growth factor receptor 3 directly regulates murine neurogenesis
Calvo CF, Fontaine RH, Soueid J, Tammela T, Makinen T, Alfaro-Cervello C, Bonnaud F, Miguez A, Benhaim L, Xu Y, Barallobre MJ, Moutkine I, Lyytikkä J, Tatlisumak T, Pytowski B, Zalc B, Richardson W, Kessaris N, Garcia-Verdugo JM, Alitalo K, Eichmann A, Thomas JL. Vascular endothelial growth factor receptor 3 directly regulates murine neurogenesis. Genes & Development 2011, 25: 831-844. PMID: 21498572, PMCID: PMC3078708, DOI: 10.1101/gad.615311.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedEnzyme-Linked Immunosorbent AssayImmunohistochemistryLymphangiogenesisMiceMice, Mutant StrainsMicroscopy, Electron, TransmissionNeovascularization, PhysiologicNeural Stem CellsNeurogenesisOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionVascular Endothelial Growth Factor Receptor-3ConceptsNeural stem cellsSubventricular zoneVEGF receptorsNeural cellsVEGFR-3Vascular endothelial growth factor (VEGF) familyEndothelial growth factor familyVascular endothelial growth factor receptor 3VEGFR-3 expressionMultipotent neural stem cellsCapillary endothelial cellsGrowth factor receptor 3Overexpression of VEGFGrowth factor familyAdult neurogenesisSVZ neurogenesisReporter miceReceptor 3NeurogenesisNeurodegenerative diseasesConditional deletionEndothelial cellsGrowth factorLigand VEGFInducible deletion
2010
Thalidomide stimulates vessel maturation and reduces epistaxis in individuals with hereditary hemorrhagic telangiectasia
Lebrin F, Srun S, Raymond K, Martin S, van den Brink S, Freitas C, Bréant C, Mathivet T, Larrivée B, Thomas JL, Arthur HM, Westermann CJ, Disch F, Mager JJ, Snijder RJ, Eichmann A, Mummery CL. Thalidomide stimulates vessel maturation and reduces epistaxis in individuals with hereditary hemorrhagic telangiectasia. Nature Medicine 2010, 16: 420-428. PMID: 20364125, DOI: 10.1038/nm.2131.Peer-Reviewed Original Research