2009
Structural basis for biosynthetic programming of fungal aromatic polyketide cyclization
Crawford JM, Korman TP, Labonte JW, Vagstad AL, Hill EA, Kamari-Bidkorpeh O, Tsai SC, Townsend CA. Structural basis for biosynthetic programming of fungal aromatic polyketide cyclization. Nature 2009, 461: 1139-1143. PMID: 19847268, PMCID: PMC2872118, DOI: 10.1038/nature08475.Peer-Reviewed Original ResearchConceptsProduct templateX-ray crystal structurePt domainsIntramolecular aldol cyclizationCo-crystal structureIterative polyketide synthaseBicyclic compoundsPolyketide cyclizationNatural productsCrystal structureAldol cyclizationDiverse structuresBiological activityBiosynthetic reactionsCyclizationPolyketide synthaseAflatoxin B1Structural basisStructureIntermediatesCompoundsReactionPotent hepatocarcinogen aflatoxin B1TemplateHepatocarcinogen aflatoxin B1
2008
Deconstruction of Iterative Multidomain Polyketide Synthase Function
Crawford J, Thomas P, Scheerer J, Vagstad A, Kelleher N, Townsend C. Deconstruction of Iterative Multidomain Polyketide Synthase Function. Science 2008, 320: 243-246. PMID: 18403714, PMCID: PMC2480491, DOI: 10.1126/science.1154711.Peer-Reviewed Original ResearchConceptsIterative polyketide synthasesIndividual catalytic domainsProduct template (PT) domainsPolyketide synthasesStarter unitCatalytic domainAromatic polyketidesBiosynthetic enzymesTemplate domainSynthase functionCarcinogen aflatoxin B1PksAMechanistic featuresEnzymePt domainsKetosynthaseDomainPolyketidesBiosynthesisSynthasesCascadeReconstitutionAccumulationFamily
2006
Identification of a starter unit acyl-carrier protein transacylase domain in an iterative type I polyketide synthase
Crawford JM, Dancy BC, Hill EA, Udwary DW, Townsend CA. Identification of a starter unit acyl-carrier protein transacylase domain in an iterative type I polyketide synthase. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 16728-16733. PMID: 17071746, PMCID: PMC1636523, DOI: 10.1073/pnas.0604112103.Peer-Reviewed Original Research