2015
Canalicular membrane MRP2/ABCC2 internalization is determined by Ezrin Thr567 phosphorylation in human obstructive cholestasis
Chai J, Cai SY, Liu X, Lian W, Chen S, Zhang L, Feng X, Cheng Y, He X, He Y, Chen L, Wang R, Wang H, Boyer JL, Chen W. Canalicular membrane MRP2/ABCC2 internalization is determined by Ezrin Thr567 phosphorylation in human obstructive cholestasis. Journal Of Hepatology 2015, 63: 1440-1448. PMID: 26212029, PMCID: PMC4686151, DOI: 10.1016/j.jhep.2015.07.016.Peer-Reviewed Original ResearchMeSH KeywordsAdultBile CanaliculiCase-Control StudiesCholestasisCytoskeletal ProteinsFemaleGallstonesGene Knockdown TechniquesHep G2 CellsHumansLiverMaleMembrane ProteinsMiddle AgedModels, BiologicalMultidrug Resistance-Associated Protein 2Multidrug Resistance-Associated ProteinsPhosphorylationProtein Kinase CReceptors, Autocrine Motility FactorRNA, MessengerThreonineConceptsObstructive cholestasisCholestatic liverMRP2 expressionMrp2 internalizationHepG2 cellsHuman obstructive cholestasisMRP2 protein expressionMembrane expressionHepatic MRP2 expressionNon-cholestatic controlsExpression of PKCαTotal protein levelsBile ductCholestatic patientsCholestasisBile acidsPatientsAbstractTextHuman liverProtein expressionProtein levelsLiverMRP2JaundiceAIMS
2001
Cl−-dependent secretory mechanisms in isolated rat bile duct epithelial units
Singh S, Mennone A, Gigliozzi A, Fraioli F, Boyer J. Cl−-dependent secretory mechanisms in isolated rat bile duct epithelial units. AJP Gastrointestinal And Liver Physiology 2001, 281: g438-g446. PMID: 11447024, DOI: 10.1152/ajpgi.2001.281.2.g438.Peer-Reviewed Original ResearchMeSH KeywordsAmmonium ChlorideAnimalsBariumBicarbonatesBile Ducts, IntrahepaticBumetanideCarbon DioxideCarrier ProteinsCell PolarityCells, CulturedChloridesColforsinEpithelial CellsHydrogen-Ion ConcentrationIon TransportMaleModels, BiologicalRatsRats, Sprague-DawleySodiumSodium-Potassium-Chloride SymportersConceptsCO2/HCOFluid secretionCotransport activityIntrahepatic bile duct epitheliumBile duct epitheliumDependent fluid secretionHigh intracellular Cl(-) concentrationIntracellular Cl- concentrationBile duct unitsApical Cl(-) secretionNH entryInitial acidification rateIntracellular cAMP concentrationNH4Cl exposureDuct epitheliumInhibitor bumetanideChannel inhibitorsCl(-) secretionCanalicular bileDuct unitsSecretionDependent secretionSecretory mechanismBumetanideForskolin
1999
Molecular regulation of hepatocellular transport systems in cholestasis
Trauner M, Meier P, Boyer J. Molecular regulation of hepatocellular transport systems in cholestasis. Journal Of Hepatology 1999, 31: 165-178. PMID: 10424299, DOI: 10.1016/s0168-8278(99)80179-2.Peer-Reviewed Original Research
1998
Intracellular pH regulation in bombesin-stimulated secretion in isolated bile duct units from rat liver
Cho W, Mennone A, Boyer J. Intracellular pH regulation in bombesin-stimulated secretion in isolated bile duct units from rat liver. American Journal Of Physiology 1998, 275: g1028-g1036. PMID: 9815033, DOI: 10.1152/ajpgi.1998.275.5.g1028.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiportersBicarbonatesBile Ducts, IntrahepaticBombesinCarrier ProteinsChloride-Bicarbonate AntiportersChloridesFluoresceinsFluorescent DyesHEPESHydrogen-Ion ConcentrationIn Vitro TechniquesMaleModels, BiologicalRatsRats, Sprague-DawleySodiumSodium-Bicarbonate SymportersSodium-Hydrogen ExchangersConceptsHCO-3 secretionBile duct unitsDuct unitsAcid-extruding mechanismsEffect of bombesinRat liverCl-/HCOBombesin-stimulated secretionRegulation of intracellularBiliary secretionNormal rat liverBombesinCholangiocytesSecretionUnderlying mechanismLiverBasalPH regulationCounterregulatoryNeuropeptidesExchange activityActivity
1996
Bile duct epithelium: frontiers in transport physiology
Boyer J. Bile duct epithelium: frontiers in transport physiology. American Journal Of Physiology 1996, 270: g1-g5. PMID: 8772494, DOI: 10.1152/ajpgi.1996.270.1.g1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBicarbonatesBileBile DuctsBiological TransportCystic Fibrosis Transmembrane Conductance RegulatorEpitheliumHumansModels, Biological
1992
Anion channels in rat liver canalicular plasma membranes reconstituted into planar lipid bilayers
Sellinger M, Weinman S, Henderson R, Zweifach A, Boyer J, Graf J. Anion channels in rat liver canalicular plasma membranes reconstituted into planar lipid bilayers. American Journal Of Physiology 1992, 262: g1027-g1032. PMID: 1377450, DOI: 10.1152/ajpgi.1992.262.6.g1027.Peer-Reviewed Original Research
1991
Effect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers
Strazzabosco M, Sakisaka S, Hayakawa T, Boyer J. Effect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers. American Journal Of Physiology 1991, 260: g58-g69. PMID: 1987808, DOI: 10.1152/ajpgi.1991.260.1.g58.Peer-Reviewed Original ResearchConceptsBiliary HCO3- concentrationUrsodeoxycholic acidRat hepatocyte coupletsBiliary pHAcid loadEffect of UDCABile duct lumenHepatocyte coupletsIsolated rat hepatocyte coupletsBiliary epitheliumHCO3- concentrationDuct lumenRemoval of ClHCO3- exchangeRat liverLiverIPRLHepatocytesHypercholeresisPerfusateBileExcretionEpitheliumAmiloride
1987
Cell membrane and transepithelial voltages and resistances in isolated rat hepatocyte couplets
Graf J, Henderson R, Krumpholz B, Boyer J. Cell membrane and transepithelial voltages and resistances in isolated rat hepatocyte couplets. The Journal Of Membrane Biology 1987, 95: 241-254. PMID: 3585979, DOI: 10.1007/bf01869486.Peer-Reviewed Original ResearchIsolated rat hepatocyte couplets in short‐term culture: Structural characteristics and plasma membrane reorganization
Gautam A, Ng O, Boyer J. Isolated rat hepatocyte couplets in short‐term culture: Structural characteristics and plasma membrane reorganization. Hepatology 1987, 7: 216-223. PMID: 3557300, DOI: 10.1002/hep.1840070203.Peer-Reviewed Original Research
1982
Cellular mechanisms of bile formation
Blitzer B, Boyer J. Cellular mechanisms of bile formation. Gastroenterology 1982, 82: 346-357. PMID: 6274732, DOI: 10.1016/0016-5085(82)90027-0.Peer-Reviewed Original Research
1977
Evaluation of a New Monkey Model for the Repeated Study of Bile Secretory Physiology
WOOD R, BAKER A, HALL A, BOYER J, MOOSSA A. Evaluation of a New Monkey Model for the Repeated Study of Bile Secretory Physiology. Annals Of Surgery 1977, 185: 349-355. PMID: 402891, PMCID: PMC1396621, DOI: 10.1097/00000658-197703000-00017.Peer-Reviewed Original ResearchConceptsBile flowSecretory physiologyMonkey modelBile salt secretion rateFinal infusion rateBile salt excretionBile salt outputHigher bile flowNew monkey modelSalt secretion rateBicarbonate outputPostoperative managementInfusion rateTaurocholate infusionSalt outputSalt excretionSecretion rateLong-term studiesRandom orderPeriod of monthsSecretinClearance