2001
Isolation of functional polarized bile duct units from mouse liver
Cho W, Mennone A, Boyer J. Isolation of functional polarized bile duct units from mouse liver. AJP Gastrointestinal And Liver Physiology 2001, 280: g241-g246. PMID: 11208546, DOI: 10.1152/ajpgi.2001.280.2.g241.Peer-Reviewed Original ResearchConceptsVasoactive intestinal peptideBile duct epitheliumMurine animal modelBile duct unitsPortal perfusionIntestinal peptideSecretory responseAnimal modelsBlunt dissectionDuct epitheliumSecretory stimuliFluid secretionDuct unitsCholangiocyte secretionFurther enzymatic digestionMouse liverSecretionMurine animalsIBDUMice
1995
Isolation of small polarized bile duct units.
Mennone A, Alvaro D, Cho W, Boyer J. Isolation of small polarized bile duct units. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 6527-6531. PMID: 7541542, PMCID: PMC41551, DOI: 10.1073/pnas.92.14.6527.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesBile DuctsBucladesineChloridesCholestasisColforsinFluoresceinsGluconatesHydrogen-Ion ConcentrationKineticsLiverMaleMicroscopy, ElectronMicroscopy, VideoRatsRats, Sprague-DawleySecretinTime FactorsConceptsBile duct unitsInterlobular bile ductsBile ductDuct unitsSmall interlobular bile ductsBile duct syndromeBile duct segmentsCholestatic liver disordersCl-/HCO3- exchange activityRat liverLiver disordersPrimary siteForskolin administrationMicroM forskolinDextran 40Duct segmentsDibutyryl cAMPUseful preparationLiverSecretionForskolinImaging techniquesDuctLuminal spaceSyndrome
1993
Effect of secretion on intracellular pH regulation in isolated rat bile duct epithelial cells.
Alvaro D, Cho W, Mennone A, Boyer J. Effect of secretion on intracellular pH regulation in isolated rat bile duct epithelial cells. Journal Of Clinical Investigation 1993, 92: 1314-1325. PMID: 8397224, PMCID: PMC288273, DOI: 10.1172/jci116705.Peer-Reviewed Original ResearchConceptsBile duct epithelial cellsDuct epithelial cellsCl-/HCO3Cell depolarizationEpithelial cellsEffect of secretinCl- channel blockerEffects of secretionsCl removalCl- channel activationRat bile duct epithelial cellsKRB mediumMaximal rateRegulation of intracellularBicarbonate secretionNormal rat liverIntracellular pH regulationMM DIDSHCO3- excretionAcid loadBDE cellsSecretinMicrofluorimetric methodRat liverHCO3- influx