Thinned-skull cranial window technique for long-term imaging of the cortex in live mice
Yang G, Pan F, Parkhurst CN, Grutzendler J, Gan WB. Thinned-skull cranial window technique for long-term imaging of the cortex in live mice. Nature Protocols 2010, 5: 201-208. PMID: 20134419, PMCID: PMC4690457, DOI: 10.1038/nprot.2009.222.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsCerebral CortexDendritesEquipment DesignIndicators and ReagentsLuminescent ProteinsMiceMice, TransgenicMicroscopy, ConfocalNeuronsPhotonsSkullSynapsesConceptsTwo-photon laser scanning microscopyHigh optical resolutionThinned-skull cranial windowImportant experimental toolOptical resolutionLiving brainCranial window techniqueLive miceLong-term imagingCranial windowExperimental toolInvasive approachAvoids exposureFunctional changesCortical structuresLongitudinal imagingPathological conditionsCortexBrainLaser scanning microscopyScanning microscopyMiceImagingMicroscopyResolution