2001
Autostimulation of the Epstein-Barr Virus BRLF1 Promoter Is Mediated through Consensus Sp1 and Sp3 Binding Sites
Ragoczy T, Miller G. Autostimulation of the Epstein-Barr Virus BRLF1 Promoter Is Mediated through Consensus Sp1 and Sp3 Binding Sites. Journal Of Virology 2001, 75: 5240-5251. PMID: 11333906, PMCID: PMC114930, DOI: 10.1128/jvi.75.11.5240-5251.2001.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesB-LymphocytesCell Line, TransformedDNA-Binding ProteinsGene DeletionGene Expression Regulation, ViralHerpesvirus 4, HumanHeterotrimeric GTP-Binding ProteinsHumansImmediate-Early ProteinsMolecular Sequence DataMutagenesis, Site-DirectedPromoter Regions, GeneticProtein BindingReceptors, Cell SurfaceSp1 Transcription FactorSp3 Transcription FactorTrans-ActivatorsTranscription FactorsViral ProteinsVirus ActivationConceptsSp1/Sp3 siteLytic cycleSp3 transcription factorsBinding of Sp1Transcriptional start siteSite-directed mutagenesisGel shift analysisBRLF1 promoterReporter-based assaysEpstein–Barr virus Rta proteinCellular Sp1Own geneConsensus Sp1Transcriptional activationCellular proteinsTranscription factorsStart siteDNA bindingOwn expressionMutagenesis studiesRta proteinSp1Reporter activityTranscription factor Zif268B cellsCD4+ T-Cell Effectors Inhibit Epstein-Barr Virus-Induced B-Cell Proliferation
Nikiforow S, Bottomly K, Miller G. CD4+ T-Cell Effectors Inhibit Epstein-Barr Virus-Induced B-Cell Proliferation. Journal Of Virology 2001, 75: 3740-3752. PMID: 11264363, PMCID: PMC114865, DOI: 10.1128/jvi.75.8.3740-3752.2001.Peer-Reviewed Original ResearchMeSH KeywordsAdultB-LymphocytesCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell DivisionCell SizeCells, CulturedEpstein-Barr Virus InfectionsEpstein-Barr Virus Nuclear AntigensFlow CytometryHerpesvirus 4, HumanHumansImmunologic MemoryLymphocyte CountReceptors, IgESerologyTacrolimusT-Lymphocyte SubsetsConceptsEpstein-Barr virusB cell proliferationT cellsB cellsB-cell lymphoproliferative diseaseRemoval of CD4Effector T cellsMemory T cellsT cell effectorsT cell surveillanceCytolytic T cellsEarly proliferative phaseCell linesImmune controlLymphoproliferative diseaseImmunodeficient hostsImmune surveillanceLymphoblastoid cell linesCD4Proliferative phaseImmortalized cell linesCD8CD23VirusInfection
1999
Role of the Epstein-Barr Virus Rta Protein in Activation of Distinct Classes of Viral Lytic Cycle Genes
Ragoczy T, Miller G. Role of the Epstein-Barr Virus Rta Protein in Activation of Distinct Classes of Viral Lytic Cycle Genes. Journal Of Virology 1999, 73: 9858-9866. PMID: 10559298, PMCID: PMC113035, DOI: 10.1128/jvi.73.12.9858-9866.1999.Peer-Reviewed Original ResearchConceptsLytic cycle genesRaji cellsEpstein–Barr virus Rta proteinEpstein-Barr virus (EBV) lytic cycleViral targetsLytic cycleVirus lytic cycleLymphoma cell line RajiBurkitt's lymphoma cell line RajiBZLF1 expressionB cell linesCell line RajiBRLF1 geneImmediate early genesInhibitory effectCycle genesViral lytic cycle genesEBVRta proteinViral genesBLRF2CellsExpressionTransactivation functionLate genesAmino Acid Substitutions Reveal Distinct Functions of Serine 186 of the ZEBRA Protein in Activation of Early Lytic Cycle Genes and Synergy with the Epstein-Barr Virus R Transactivator
Francis A, Ragoczy T, Gradoville L, Heston L, El-Guindy A, Endo Y, Miller G. Amino Acid Substitutions Reveal Distinct Functions of Serine 186 of the ZEBRA Protein in Activation of Early Lytic Cycle Genes and Synergy with the Epstein-Barr Virus R Transactivator. Journal Of Virology 1999, 73: 4543-4551. PMID: 10233912, PMCID: PMC112494, DOI: 10.1128/jvi.73.6.4543-4551.1999.Peer-Reviewed Original ResearchConceptsLytic cycle genesLatent virusZEBRA proteinOverexpression of RtaEpstein-Barr virusLatent EBV genomeBRLF1 expressionEarly lytic cycle genesAmino acid substitutionsEBV genomeBRLF1BRLF1 promoterCycle genesAcid substitutionsVirusEpstein-Barr virus R transactivatorSerine 186Distinct phenotypesLytic life cycleR transactivatorActivationDifferent amino acid substitutionsResponse elementS186Expression
1998
A Functionally Distinct TATA Box Required for Late Progression through the Epstein-Barr Virus Life Cycle
Serio T, Cahill N, Prout M, Miller G. A Functionally Distinct TATA Box Required for Late Progression through the Epstein-Barr Virus Life Cycle. Journal Of Virology 1998, 72: 8338-8343. PMID: 9733880, PMCID: PMC110205, DOI: 10.1128/jvi.72.10.8338-8343.1998.Peer-Reviewed Original ResearchThe Epstein-Barr Virus Rta Protein Activates Lytic Cycle Genes and Can Disrupt Latency in B Lymphocytes
Ragoczy T, Heston L, Miller G. The Epstein-Barr Virus Rta Protein Activates Lytic Cycle Genes and Can Disrupt Latency in B Lymphocytes. Journal Of Virology 1998, 72: 7978-7984. PMID: 9733836, PMCID: PMC110133, DOI: 10.1128/jvi.72.10.7978-7984.1998.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceB-LymphocytesCell LineChloramphenicol O-AcetyltransferaseDNA PrimersDNA ReplicationDNA-Binding ProteinsGene Expression Regulation, ViralHerpesvirus 4, HumanHumansImmediate-Early ProteinsPromoter Regions, GeneticTrans-ActivatorsTranscription FactorsViral ProteinsVirus LatencyConceptsEpstein-Barr virusLytic cycle genesB lymphocytesEpstein–Barr virus Rta proteinEpithelial cellsLytic cycleDisruption of latencyViral lytic cycleB cell linesEBV entryImmediate early viral genesBZLF1LymphocytesCycle genesExpression of RTARTA functionBRLF1Rta proteinDownstream targetsViral genesViral DNA replicationExpressionCellsA viral gene that activates lytic cycle expression of Kaposi’s sarcoma-associated herpesvirus
Sun R, Lin S, Gradoville L, Yuan Y, Zhu F, Miller G. A viral gene that activates lytic cycle expression of Kaposi’s sarcoma-associated herpesvirus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10866-10871. PMID: 9724796, PMCID: PMC27987, DOI: 10.1073/pnas.95.18.10866.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCloning, MolecularDNA PrimersDNA, ComplementaryGene Expression Regulation, NeoplasticGenes, ViralHerpesvirus 4, HumanHerpesvirus 8, HumanHumansMolecular Sequence DataSarcoma, KaposiSequence Homology, Amino AcidTranscription, GeneticTumor Cells, CulturedVirus ActivationConceptsEpstein-Barr virusKaposi's sarcoma-associated herpesvirusSarcoma-associated herpesvirusSmall viral capsid antigenPathogenesis of KSHVLytic cycle gene expressionViral capsid antigenLatent viral genomeCapsid antigenLytic cycle genesInterleukin-6Immediate early genesViral cytokineEarly lytic genesLytic genesHerpesvirusVirusViral genesViral genomeExpressionGene expressionCytokinesPathogenesisCycle genesGenes
1997
The Locus of Epstein–Barr Virus Terminal Repeat Processing Is Bound with Enhanced Affinity by Sp1 and Sp3
Spain T, Sun R, Miller G. The Locus of Epstein–Barr Virus Terminal Repeat Processing Is Bound with Enhanced Affinity by Sp1 and Sp3. Virology 1997, 237: 137-147. PMID: 9344916, DOI: 10.1006/viro.1997.8770.Peer-Reviewed Original ResearchConceptsRecombination eventsRepeat binding proteinMinimal binding siteAntibody supershift assaysRepeat processingSp1 sitesCellular proteinsLarge internal repeatRecombinogenic regionsInternal repeatsSp1Supershift assaysRecombinant proteinsTerminal repeatBinding proteinLytic cycle inductionEBV lytic cycle inductionCycle inductionProteinAffinity of bindingBinding sitesRepeatsSite 1DNASp3
1995
Transmissible Retrovirus in Epstein-Burr Virus-Producer B95-8 Cells
Sun R, Grogan E, Shedd D, Bykovsky A, Kushnaryov V, Grossberg S, Miller G. Transmissible Retrovirus in Epstein-Burr Virus-Producer B95-8 Cells. Virology 1995, 209: 374-383. PMID: 7778272, DOI: 10.1006/viro.1995.1269.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBetaretrovirusB-LymphocytesCallithrixCell LineCloning, MolecularDNA PrimersDNA, ComplementaryGene LibraryGene Products, gagGenes, gagHerpesvirus 4, HumanHumansMicroscopy, ElectronMolecular Sequence DataPolymerase Chain ReactionRepetitive Sequences, Nucleic AcidSaimiriT-LymphocytesVirus ReplicationConceptsEpstein-Barr virusB95-8 cellsAbsence of EBVCell linesB95-8 isolateB95-8 strainMarmoset cell linesHuman lymphoid cell linesT cell linesLymphoid cell linesType D retrovirusHuman BLymphocyte immortalizationInfectious virusViral replicationHuman isolatesGag regionBiologic propertiesTransmissible retrovirusUltrastructural appearanceD retrovirusesAmino acid changesVirusSMRVGag sequences
1992
Detection of Epstein‐Barr virus in the brain by the polymerase chain reaction
Pedneault L, Katz B, Miller G. Detection of Epstein‐Barr virus in the brain by the polymerase chain reaction. Annals Of Neurology 1992, 32: 184-192. PMID: 1324632, DOI: 10.1002/ana.410320210.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusEBV DNAPolymerase chain reactionRole of EBVChain reactionCentral nervous system syndromeDiverse neurological syndromesKidney transplant recipientsHerpes group virusesTransplant recipientsMetabolic encephalopathiesBrain biopsyImmunodeficiency syndromeNeurological syndromeImmunodeficient hostsEBV genomeBrain specimensViral isolationPatientsGroup virusesSerological testsSyndromeBrainVirusEncephalitis
1990
Differences in the extent of activation of Epstein-Barr virus replicative gene expression among four nonproducer cell lines stably transformed by OriP/BZLF1 plasmids
Gradoville L, Grogan E, Taylor N, Miller G. Differences in the extent of activation of Epstein-Barr virus replicative gene expression among four nonproducer cell lines stably transformed by OriP/BZLF1 plasmids. Virology 1990, 178: 345-354. PMID: 2171186, DOI: 10.1016/0042-6822(90)90331-k.Peer-Reviewed Original ResearchConceptsCell linesEffects of mutationsStable cell linesExtent of activationProtein functionCellular genesGene productsExtrachromosomal plasmidsGene expressionNonproducer cell linesExpression vectorEarly antigenEarly genesGenesLymphoid cell linesCellular subclonesEBV early genesReplicative gene expressionX50-7 cellsZEBRA proteinPlasmidZebraBZLF1 gene productLatent EBVEBV genesInhibition of specific binding of EBNA 1 to DNA by Murine Monoclonal and certain human polyclonal antibodies
Orlowski R, Polvino-Bodnar M, Hearing J, Miller G. Inhibition of specific binding of EBNA 1 to DNA by Murine Monoclonal and certain human polyclonal antibodies. Virology 1990, 176: 638-642. PMID: 2161154, DOI: 10.1016/0042-6822(90)90036-q.Peer-Reviewed Original ResearchConceptsEBNA-1 proteinGel mobility shift experimentsMobility shift experimentsSequence-specific DNARecognition of DNAEBNA-1Binding of DNASpecific bindingLac promoterDNA bindingMonoclonal antibodiesDimerization siteSpecific DNANonfusion proteinBurkitt's lymphoma cellsShift experimentsMolecular massEscherichia coliProteinDNASame monoclonal antibodyBindingPolyclonal antibodiesEB virusLymphoma cellsTopological Effects of EBNA 1 on oriP
Orlowski R, Miller G. Topological Effects of EBNA 1 on oriP. Advances In Experimental Medicine And Biology 1990, 278: 115-124. PMID: 1963028, DOI: 10.1007/978-1-4684-5853-4_12.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigens, ViralBase SequenceBinding SitesDeoxyribonuclease IV (Phage T4-Induced)DNA, SuperhelicalDNA, ViralEndodeoxyribonucleasesEpstein-Barr Virus Nuclear AntigensEscherichia coliEscherichia coli ProteinsGenes, ViralHerpesvirus 4, HumanMolecular Sequence DataNucleic Acid ConformationPlasmidsRecombinant ProteinsRepetitive Sequences, Nucleic AcidTransformation, Bacterial
1988
Polymorphisms of the region of the Epstein-Barr virus genome which disrupts latency
Jenson H, Miller G. Polymorphisms of the region of the Epstein-Barr virus genome which disrupts latency. Virology 1988, 165: 549-564. PMID: 2841800, DOI: 10.1016/0042-6822(88)90599-5.Peer-Reviewed Original ResearchTHE FREQUENCY OF EPSTEIN-BARR VIRUS INFECTION AND ASSOCIATED LYMPHOPROLIFERATIVE SYNDROME AFTER TRANSPLANTATION AND ITS MANIFESTATIONS IN CHILDREN
Ho M, Jaffe R, Miller G, Breinig M, Dummer J, Makowka L, Atchison R, Karrer F, Nalesnik M, Starzl T. THE FREQUENCY OF EPSTEIN-BARR VIRUS INFECTION AND ASSOCIATED LYMPHOPROLIFERATIVE SYNDROME AFTER TRANSPLANTATION AND ITS MANIFESTATIONS IN CHILDREN. Transplantation 1988, 45: 719-726. PMID: 2833828, PMCID: PMC2993427, DOI: 10.1097/00007890-198804000-00011.Peer-Reviewed Original ResearchConceptsLymphoproliferative syndromePrimary infectionEBV infectionTransplant recipientsEpstein-Barr virus infectionMonoclonal B-cell lymphomaPediatric liver recipientsPrimary EBV infectionPediatric transplant recipientsEpstein-Barr virusEBV nuclear antigenB-cell lymphomaLiver recipientsPediatric EBVCyclosporine monitoringEBV DNAClinical typesPediatric casesSecond syndromeVirus infectionCell lymphomaAdditional casesGreater riskInfectionNuclear antigen
1987
Novel nuclear antigens recognized by human sera in lymphocytes latently infected by Epstein-Barr virus
Rowe D, Farrell P, Miller G. Novel nuclear antigens recognized by human sera in lymphocytes latently infected by Epstein-Barr virus. Virology 1987, 156: 153-162. PMID: 3027963, DOI: 10.1016/0042-6822(87)90446-6.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, ViralCell NucleusEpstein-Barr Virus Nuclear AntigensHerpesvirus 4, HumanHumansLymphocytesMolecular WeightConceptsEBNA seraAntigen complexX50-7Immortalization of lymphocytesEpstein-Barr virusEBNA INovel nuclear antigenIV groupNew antigensNuclear antigenLymphocytesCell clonesWestern immunoblottingSerumCell linesEBVAntigenMolecular weight proteinsHuman serumHigh molecular weight proteinsRajiVirusViral genomeGroupWeight proteins
1986
Primary central nervous system lymphoma related to Epstein‐Barr virus in a patient with acquired immune deficiency syndrome
Rosenberg N, Hochberg F, Miller G, Kleinschmidt‐DeMasters B. Primary central nervous system lymphoma related to Epstein‐Barr virus in a patient with acquired immune deficiency syndrome. Annals Of Neurology 1986, 20: 98-102. PMID: 3017188, DOI: 10.1002/ana.410200118.Peer-Reviewed Original ResearchConceptsCentral nervous system lymphomaEpstein-Barr virus infectionNervous system lymphomaImmune deficiency syndromeSystem lymphomaDeficiency syndromeVirus infectionPrimary central nervous system lymphomaEpstein-Barr virus genomeEpstein-Barr virusCentral nervous systemPrimary lymphomaNervous systemTumor tissueLymphomaPatientsSyndromeInfectionAcid preparationsHybridization studiesDeoxyribonucleic acid preparationsVirus genomeK fragmentAnimal model for Epstein-Barr lymphoma
MILLER G. Animal model for Epstein-Barr lymphoma. Nature 1986, 319: 626-626. PMID: 3005864, DOI: 10.1038/319626c0.Peer-Reviewed Original Research
1985
Identification and expression of a nuclear antigen from the genomic region of the Jijoye strain of Epstein-Barr virus that is missing in its nonimmortalizing deletion mutant, P3HR-1.
Rowe D, Heston L, Metlay J, Miller G. Identification and expression of a nuclear antigen from the genomic region of the Jijoye strain of Epstein-Barr virus that is missing in its nonimmortalizing deletion mutant, P3HR-1. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 7429-7433. PMID: 2997790, PMCID: PMC391358, DOI: 10.1073/pnas.82.21.7429.Peer-Reviewed Original ResearchConceptsChronic active EBV infectionActive EBV infectionEpstein-Barr virusJijoye strainEBV infectionEBV DNACertain patientsP3HR-1Human antiseraVirus strainsNuclear antigenHr-1JijoyeVirus deletion mutantsLtk- cellsMouse Ltk- cellsImmortalization processPatientsGene transferNeoantigensLymphocytesInfectionAntigenAn identification of a transforming region of Epstein-Barr viral DNA cannot be confirmed
Miller G, Sugden B. An identification of a transforming region of Epstein-Barr viral DNA cannot be confirmed. Virology 1985, 143: 478-484. PMID: 2998023, DOI: 10.1016/0042-6822(85)90387-3.Peer-Reviewed Original Research