1983
Acidification of macrophage and fibroblast endocytic vesicles in vitro.
Galloway C, Dean G, Marsh M, Rudnick G, Mellman I. Acidification of macrophage and fibroblast endocytic vesicles in vitro. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 3334-3338. PMID: 6190176, PMCID: PMC394037, DOI: 10.1073/pnas.80.11.3334.Peer-Reviewed Original ResearchConceptsAcidic prelysosomal compartmentEndosome-lysosome fusionATP-dependent acidificationF0-ATPaseEndocytic vesiclesPrelysosomal compartmentAddition of ATPPrelysosomal vacuolesMitochondrial F1LysosomesEndosomesSecretory granulesCultured macrophagesN-ethylmaleimideInternal pHPercoll density gradientsAcidificationPH gradientFibroblastsCompartmentsOrganellesInhibitorsPermeant anionsFluorescein-dextranVacuoles
1982
Bioenergetics of serotonin transport by membrane vesicles derived from platelet dense granules.
Fishkes H, Rudnick G. Bioenergetics of serotonin transport by membrane vesicles derived from platelet dense granules. Journal Of Biological Chemistry 1982, 257: 5671-5677. PMID: 7068613, DOI: 10.1016/s0021-9258(19)83830-9.Peer-Reviewed Original ResearchConceptsPlatelet dense granulesMembrane vesiclesSerotonin transport activitySoluble enzymatic activityPresence of ATPCarbonyl cyanide p-trifluoromethoxyphenylhydrazoneAddition of ATPOsmotic lysatesTransport activityCyanide p-trifluoromethoxyphenylhydrazoneEnzymatic activityDensity gradient centrifugationVesiclesSerotonin transportVesicle interiorDense granulesMembrane potentialP-trifluoromethoxyphenylhydrazoneGradient centrifugationATPAssay mediumAdenine nucleotidesBioenergeticsNucleotidesGranules