2005
Construction and Characterization of Genetically Modified Synechocystis sp. PCC 6803 Photosystem II Core Complexes Containing Carotenoids with Shorter π-Conjugation than β-Carotene*
Bautista J, Tracewell C, Schlodder E, Cunningham F, Brudvig G, Diner B. Construction and Characterization of Genetically Modified Synechocystis sp. PCC 6803 Photosystem II Core Complexes Containing Carotenoids with Shorter π-Conjugation than β-Carotene*. Journal Of Biological Chemistry 2005, 280: 38839-38850. PMID: 16159754, DOI: 10.1074/jbc.m504953200.Peer-Reviewed Original ResearchMeSH KeywordsBeta CaroteneCarotenoidsCationsChlorophyllChromatographyChromatography, High Pressure LiquidElectronsGene DeletionLightManganeseModels, ChemicalModels, MolecularMutationOxidation-ReductionOxidoreductasesOxygenPhotosynthetic Reaction Center Complex ProteinsPhotosystem II Protein ComplexPigmentationRhodobacter capsulatusSpectrophotometrySpectrophotometry, InfraredSynechocystisTemperatureTime FactorsTyrosineConceptsPhytoene desaturase geneII core complexesDesaturase genePS II core complexesSynechocystis spCore complexPS II assemblyCarotene desaturase genePhotosystem II core complexPCC 6803Rhodobacter capsulatusWild typeMutant strainRedox functionPhotosystem IISecondary electron transfer pathwayGenesElectron transfer pathwayLight-induced formationCarotenoidsSpChlorophyllConjugated pi-electron systemPathwayComplexes
1996
Isolation and Characterization of Spinach Photosystem II Membrane-Associated Catalase and Polyphenol Oxidase †
Sheptovitsky Y, Brudvig G. Isolation and Characterization of Spinach Photosystem II Membrane-Associated Catalase and Polyphenol Oxidase †. Biochemistry 1996, 35: 16255-16263. PMID: 8973199, DOI: 10.1021/bi9613842.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsCatalaseCatechol OxidaseChloroplastsChromatography, High Pressure LiquidChromatography, Ion ExchangeCopperElectrophoresis, Polyacrylamide GelHot TemperatureIntracellular MembranesKineticsMolecular WeightOxygenOxygen ConsumptionPeptide MappingPhotosynthetic Reaction Center Complex ProteinsPhotosystem II Protein ComplexSpectrophotometrySpinacia oleraceaSubstrate SpecificityConceptsPSII membranesCentre of PSIIUV-visible absorptionO2 evolution activityThylakoid membranesSpinach thylakoid membranesAnion-exchange chromatographyMembrane-associated catalaseHeme enzymesBatch adsorptionHeme catalasesMild heat treatmentHeat treatmentMolecular weightAnion-exchange beadsNative molecular weightPPOIrreversible aggregationGel filtrationFurther manipulationAdsorptionAmino acid compositionTriazoleMembraneChromatography