2014
Effects of biomechanical forces on signaling in the cortical collecting duct (CCD)
Carrisoza-Gaytan R, Liu Y, Flores D, Else C, Lee HG, Rhodes G, Sandoval RM, Kleyman TR, Lee FY, Molitoris B, Satlin LM, Rohatgi R. Effects of biomechanical forces on signaling in the cortical collecting duct (CCD). American Journal Of Physiology. Renal Physiology 2014, 307: f195-f204. PMID: 24872319, PMCID: PMC4152160, DOI: 10.1152/ajprenal.00634.2013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutocrine CommunicationCell LineDinoprostoneEnzyme ActivationExtracellular Signal-Regulated MAP KinasesFurosemideInjectionsIon TransportKidney CortexKidney Tubules, CollectingMechanotransduction, CellularMiceMicroscopy, Fluorescence, MultiphotonP38 Mitogen-Activated Protein KinasesParacrine CommunicationPhosphorylationPotassiumRatsRats, Sprague-DawleySodium ChlorideStress, MechanicalTime FactorsConceptsTubular fluid flow rateP-p38Biomechanical forcesCircumferential stretchIntravital microscopic approachP-ERK expressionFurosemide injectionPGE2 releaseIntracellular MAPKK secretionCollagen type IVCell culture modelTubular diameterEx vivoPGE2Fluid shear stressUnstretched controlsCCD cellsType IVP38 activationCulture modelMAPK signalingVivoPhysiological forcesCation transportProteomic identification of 14‐3‐3ϵ as a linker protein between pERK1/2 inhibition and BIM upregulation in human osteosarcoma cells
Kim KO, Hsu AC, Lee HG, Patel N, Chandhanayingyong C, Hickernell T, Lee F. Proteomic identification of 14‐3‐3ϵ as a linker protein between pERK1/2 inhibition and BIM upregulation in human osteosarcoma cells. Journal Of Orthopaedic Research® 2014, 32: 848-854. PMID: 24536031, DOI: 10.1002/jor.22598.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsApoptosisApoptosis Regulatory ProteinsBcl-2-Like Protein 11Cell Line, TumorExtracellular Signal-Regulated MAP KinasesFlavonoidsHumansMembrane ProteinsOsteosarcomaProteomicsProto-Oncogene ProteinsRNA, Small InterferingUp-RegulationConceptsPERK1/2 inhibitionHuman osteosarcoma cellsBim expressionSubset of patientsOsteosarcoma cellsConventional cytotoxic agentsConventional cytotoxic treatmentAnti-cancer effectsInhibition of pERK1/2Pro-apoptotic mechanismsMultifocal osteosarcomaMultimodality chemotherapyCytotoxic treatmentUpregulation of BimOsteosarcoma treatmentOsteosarcoma cell deathCytotoxic agentsExpression of BimPotential mediatorsTreatment of PD98059UpregulationTreatmentPD98059 treatmentInhibitionCell death
2011
Actin and ERK1/2-CEBPβ signaling mediates phagocytosis-induced innate immune response of osteoprogenitor cells
Lee HG, Minematsu H, Kim KO, Aydemir A, Shin MJ, Nizami SA, Chung KJ, Hsu AC, Jacobs CR, Lee FY. Actin and ERK1/2-CEBPβ signaling mediates phagocytosis-induced innate immune response of osteoprogenitor cells. Biomaterials 2011, 32: 9197-9206. PMID: 21899882, PMCID: PMC3193180, DOI: 10.1016/j.biomaterials.2011.08.059.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdhesivenessAnimalsBenzimidazolesBone and BonesCCAAT-Enhancer-Binding Protein-betaCyclooxygenase 2Cytochalasin DExtracellular Signal-Regulated MAP KinasesGene Expression RegulationHumansImmunity, InnateInflammationInterleukin-6MAP Kinase Signaling SystemMiceModels, BiologicalOsteogenesisOsteolysisPhagocytosisProtein Kinase InhibitorsSignal TransductionSkullStem CellsTime FactorsTitaniumConceptsInflammatory osteolysisInflammatory responseMacrophage-mediated inflammatory responsesKey inflammatory pathwaysSuitable therapeutic targetInflammatory gene expressionInnate immune responseOsteoprogenitor cellsInflammatory cascadeInflammatory pathwaysImmune responseTherapeutic targetHost bone-implant interfaceAZD6244 treatmentIntracellular mechanismsBone-implant interfaceBone formationCellular mechanismsERK pathwayInflammationΒ pathwayOsteoclastogenesisOsteolysisIntracellular signalingImplant osteointegration
2007
ERK Signaling Regulates Macrophage Colony‐Stimulating Factor Expression Induced by Titanium Particles in MC3T3.E1 Murine Calvarial Preosteoblastic Cells
SEO SW, LEE D, CHO SK, KIM AD, MINEMATSU H, AYDEMIR A, GELLER JA, MACAULAY W, YANG J, LEE F. ERK Signaling Regulates Macrophage Colony‐Stimulating Factor Expression Induced by Titanium Particles in MC3T3.E1 Murine Calvarial Preosteoblastic Cells. Annals Of The New York Academy Of Sciences 2007, 1117: 151-158. PMID: 18056041, DOI: 10.1196/annals.1402.027.Peer-Reviewed Original ResearchConceptsM-CSF expressionExtracellular signal-regulated kinase (ERK) pathwaySignal-regulated kinase pathwayPreosteoblastic cellsSignal transduction pathwaysSpecific ERK inhibitorReverse transcriptase-polymerase chain reactionEnzyme-linked immunosorbent assayPreosteoclast proliferationTransduction pathwaysKinase pathwayMolecular mechanismsMacrophage colony-stimulating factorCellular environmentERK inhibitorPeriprosthetic osteolysisM-CSF productionNuclear factor kappa beta ligandCell populationsBeta ligandSignificant clinical problemTranscriptase-polymerase chain reactionExpressionDose-dependent mannerColony-stimulating factor