2008
A recombinant West Nile virus envelope protein vaccine candidate produced in Spodoptera frugiperda expresSF+ cells
Bonafé N, Rininger JA, Chubet RG, Foellmer HG, Fader S, Anderson JF, Bushmich SL, Anthony K, Ledizet M, Fikrig E, Koski RA, Kaplan P. A recombinant West Nile virus envelope protein vaccine candidate produced in Spodoptera frugiperda expresSF+ cells. Vaccine 2008, 27: 213-222. PMID: 18996430, PMCID: PMC2651515, DOI: 10.1016/j.vaccine.2008.10.046.Peer-Reviewed Original ResearchConceptsVaccine candidatesClinical adverse effectsProtein vaccine candidateRecombinant vaccine candidateWNV vaccineAntibody titersHumoral immunityProtective efficacySafe vaccineWNV infectionChallenge modelVaccine antigensAnimal modelsViral protectionHigh dosesProtein antigensAdverse effectsImmunogenicitySerum-free cultureAntigenCell linesNaïve foalsVaccineAluminum hydroxideInfection
2007
Antibodies Targeting Linear Determinants of the Envelope Protein Protect Mice against West Nile Virus
Ledizet M, Kar K, Foellmer HG, Bonafé N, Anthony KG, Gould LH, Bushmich SL, Fikrig E, Koski RA. Antibodies Targeting Linear Determinants of the Envelope Protein Protect Mice against West Nile Virus. The Journal Of Infectious Diseases 2007, 196: 1741-1748. PMID: 18190253, DOI: 10.1086/523654.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, ViralAntibody AffinityChlorocebus aethiopsDrosophilaEpitopesFemaleFluorescent Antibody TechniqueHorsesImmunization, PassiveImmunoglobulinsMiceMice, Inbred C3HMolecular Sequence DataPeptide FragmentsVero CellsViral Envelope ProteinsWest Nile FeverWest Nile virusConceptsE proteinMultiple flavivirus infectionsVirus-neutralizing antibodiesE protein epitopesFlavivirus envelope proteinProtect miceWest Nile virusFlavivirus infectionLethal challengeProtective antibodiesWest Nile virus E proteinProtective epitopesVirus E proteinRelated flavivirusesImmunoglobulinAntibodiesNile virusLinear determinantsEpitopesEnvelope proteinProtein epitopesMiceHost cell membraneWNVPeptides
2005
A recombinant envelope protein vaccine against West Nile virus
Ledizet M, Kar K, Foellmer HG, Wang T, Bushmich SL, Anderson JF, Fikrig E, Koski RA. A recombinant envelope protein vaccine against West Nile virus. Vaccine 2005, 23: 3915-3924. PMID: 15917113, DOI: 10.1016/j.vaccine.2005.03.006.Peer-Reviewed Original ResearchDetection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens
Magnarelli L, Fikrig E. Detection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens. Research In Veterinary Science 2005, 79: 99-103. PMID: 15924926, DOI: 10.1016/j.rvsc.2004.11.009.Peer-Reviewed Original Research
2002
A Recombinant Envelope Protein-Based Enzyme-Linked Immunosorbent Assay for West Nile Virus Serodiagnosis
Wang T, Magnarelli LA, Anderson JF, Gould LH, Bushmich SL, Wong SJ, Fikrig E. A Recombinant Envelope Protein-Based Enzyme-Linked Immunosorbent Assay for West Nile Virus Serodiagnosis. Vector-Borne And Zoonotic Diseases 2002, 2: 105-109. PMID: 12653304, DOI: 10.1089/153036602321131904.Peer-Reviewed Original Research
2001
Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Oliver JH, Fikrig E. Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis. American Journal Of Veterinary Research 2001, 62: 1365-9. PMID: 11560261, DOI: 10.2460/ajvr.2001.62.1365.Peer-Reviewed Original ResearchConceptsIgG antibodiesSerum samplesHGE agentGranulocytic ehrlichiosisClient-owned dogsDog serum samplesHuman granulocytic ehrlichiosis (HGE) agentEnzyme-linked immunosorbentPositive resultsWestern blot analysisTick-infested areasEhrlichia phagocytophila genogroupTotal immunoglobulinHorse serum samplesPromyelocytic leukemia cellsClinical relevanceHuman promyelocytic leukemia cellsProtein antigensIgMELISADogsLeukemia cellsAntibodiesBlot analysisAntigenEvaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Fikrig E. Evaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses. American Journal Of Veterinary Research 2001, 62: 29-32. PMID: 11197555, DOI: 10.2460/ajvr.2001.62.29.Peer-Reviewed Original ResearchConceptsEquine samplesPolyvalent ELISAPolyvalent enzyme-linked immunosorbentGranulocytic ehrlichiosisIFA stainingEquine serum samplesE risticiiEhrlichia canisCanine serum samplesCanine samplesSpecific recombinant antigensE equiHorsesEhrlichiosisPercentage of samplesIndirect fluorescent antibody stainingRecombinant antigensDogsWhole cell antigenCanisEquiELISA resultsSerum samplesPresent studyFluorescent antibody staining
2000
Serologic confirmation of Ehrlichia equi and Borrelia burgdorferi infections in horses from the northeastern United States.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Padula SJ, Fikrig E. Serologic confirmation of Ehrlichia equi and Borrelia burgdorferi infections in horses from the northeastern United States. Journal Of The American Veterinary Medical Association 2000, 217: 1045-50. PMID: 11019714, DOI: 10.2460/javma.2000.217.1045.Peer-Reviewed Original ResearchConceptsB burgdorferiClinical signsGranulocytic ehrlichiosisSerum samplesDetectable serum antibodiesImmunoblot analysisIndirect fluorescent antibody stainingBorrelial antibodiesTick-infested areasRecombinant protein antigensProspective studyDetectable antibodiesSerum antibodiesSerologic confirmationFluorescent antibody stainingE equiAntibody reactivityClinical relevanceEtiologic agentClinical diagnosisEqui antibodiesProtein antigensEhrlichia equiELISAAntibodies
1999
Serologic testing of horses for granulocytic ehrlichiosis, using indirect fluorescent antibody staining and immunoblot analysis.
Magnarelli L, Van Andel A, Ijdo J, Heimer R, Fikrig E. Serologic testing of horses for granulocytic ehrlichiosis, using indirect fluorescent antibody staining and immunoblot analysis. American Journal Of Veterinary Research 1999, 60: 631-5. PMID: 10328436, DOI: 10.2460/ajvr.1999.60.05.631.Peer-Reviewed Original Research
1998
Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays
Magnarelli L, IJdo J, Dumler J, Heimer R, Fikrig E. Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays. The Journal Of Infectious Diseases 1998, 178: 1835-1838. PMID: 9815246, DOI: 10.1086/314516.Peer-Reviewed Original ResearchConceptsNCH-1 strainIndirect IFAHuman granulocytic ehrlichiosis (HGE) agentHuman granulocytic ehrlichiosisWestern blot analysisInfected neutrophilsPromyelocytic leukemia cellsHuman promyelocytic leukemia cellsAntibody detectionGranulocytic ehrlichiosisEhrlichia equiDifferent strainsHuman isolatesLeukemia cellsSerumImmunodiagnostic assaysBlot analysisGranulocytic ehrlichiaeIFA analysisHuman serumIFAEhrlichiaeAssaysSeropositivityPatients
1993
Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi
Fikrig E, Magnarelli LA, Chen M, Anderson JF, Flavell RA. Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi. Journal Of Clinical Microbiology 1993, 31: 2451-2455. PMID: 7691874, PMCID: PMC265777, DOI: 10.1128/jcm.31.9.2451-2455.1993.Peer-Reviewed Original Research