1996
Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue
Das S, Shraga D, Gannon C, Lam TT, Feng S, Brunet LR, Telford SR, Barthold SW, Flavell RA, Fikrig E. Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue. Research In Microbiology 1996, 147: 739-751. PMID: 9296108, DOI: 10.1016/s0923-2508(97)85121-2.Peer-Reviewed Original ResearchConceptsSubstrate-binding proteinGram-negative bacteriaPeriplasmic substrate-binding proteinB. burgdorferiProtein homologueOligopeptide permeaseChromosomal genesConsiderable homologyP30 expressionB. burgdorferi-infected miceMolecular massSensu latoAmino acidsC3H/HeN miceLyme diseaseB. burgdorferi sensu latoBurgdorferi-infected miceSubset of patientsP30 geneMurine Lyme borreliosisGenesLate-stage infectionRegion 36Recombinant p30Anti-p30 serum
1994
Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease
Lam TT, Nguyen TP, Montgomery RR, Kantor FS, Fikrig E, Flavell RA. Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease. Infection And Immunity 1994, 62: 290-298. PMID: 8262642, PMCID: PMC186099, DOI: 10.1128/iai.62.1.290-298.1994.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceBlotting, WesternBorrelia burgdorferi GroupCloning, MolecularCodonFluorescent Antibody TechniqueGenes, BacterialHumansLipoproteinsLyme DiseaseMolecular Sequence DataMolecular WeightOperonRegulatory Sequences, Nucleic AcidRestriction MappingSequence AlignmentSequence Homology, Nucleic AcidSolubilityConceptsOspE genesMolecular massSignal peptidase IIConsensus cleavage sequenceTranscriptional unitsLeader sequenceCommon promoterBp downstreamOuter surface proteinsProtein EStop codonSurface lipoproteinsLabeling showBorrelia burgdorferiGenesHydrophobic domainCleavage sequenceSurface proteinsAmino acidsPeptidase IIProteinOuter surface protein EGel electrophoresisNucleotidesImmunofluorescence studies