2013
Influence of Chitosan Concentration on Cell Viability and Proliferation in Vitro by Changing Film Topography
Martín-López E, Nieto-Díaz M, Nieto-Sampedro M. Influence of Chitosan Concentration on Cell Viability and Proliferation in Vitro by Changing Film Topography. Journal Of Applied Biomaterials & Functional Materials 2013, 11: 151-158. PMID: 23413128, DOI: 10.5301/jabfm.2012.10449.Peer-Reviewed Original ResearchConceptsChitosan filmsAtomic force microscopyFilm topographyTissue engineering applicationsChitosan concentrationPolymer surface topographyNeural tissue engineeringEnvironmental scanning electron microscopyScanning electron microscopyEngineering applicationsSurface topographyTissue engineeringAFM profilesFilmsForce microscopyTopography changesVitro biological propertiesChitosan solutionElectron microscopyChitosan polymerAlkaline precipitationNatural polysaccharidesTopographyPropertiesBridgeAgar-based bridges as biocompatible candidates to provide guide cues in spinal cord injury repair.
Martín-López E, Darder M, Ruiz-Hitzky E, Nieto Sampedro M. Agar-based bridges as biocompatible candidates to provide guide cues in spinal cord injury repair. Bio-Medical Materials And Engineering 2013, 23: 405-21. PMID: 23988711, DOI: 10.3233/bme-130763.Peer-Reviewed Original ResearchConceptsEnvironmental scanning electron microscopyScanning electron microscopyPorous structureBridge materialsBiomaterial propertiesCell ingrowthChannel linearityMixture of agarGrowth surfaceXanthan gumStability of gelsElectron microscopyEmpty channelsBiopolymer structuresPolysulfoneMatrix scaffoldsBiocompatible candidateΚ-carrageenanClosed contactBridgeMaterialsGel mixtureMixtureSpinal cord injury repairStability
2012
Chitosan, Gelatin and Poly(L-Lysine) Polyelectrolyte-Based Scaffolds and Films for Neural Tissue Engineering
Martín-López E, Alonso FR, Nieto-Díaz M, Nieto-Sampedro M. Chitosan, Gelatin and Poly(L-Lysine) Polyelectrolyte-Based Scaffolds and Films for Neural Tissue Engineering. Journal Of Biomaterials Science Polymer Edition 2012, 23: 207-232. PMID: 21192838, DOI: 10.1163/092050610x546426.Peer-Reviewed Original ResearchConceptsNeural tissue engineeringTissue engineeringG filmsSmall pore sizeThermal resistancePore sizeSuitable materialBiomaterial implantsFilmsBest mixturePromising candidateEngineeringDorsal root gangliaDifferent physicochemical approachesDistinct electrostatic interactionsTraumatic nerve damageSpindle cell morphologyGel morphologyGlial cell growthNerve damage
2010
Differential Adhesiveness and Neurite-promoting Activity for Neural Cells of Chitosan, Gelatin, and Poly-l-Lysine Films
Martín-López E, Nieto-Díaz M, Nieto-Sampedro M. Differential Adhesiveness and Neurite-promoting Activity for Neural Cells of Chitosan, Gelatin, and Poly-l-Lysine Films. Journal Of Biomaterials Applications 2010, 26: 791-809. PMID: 20876636, DOI: 10.1177/0885328210379928.Peer-Reviewed Original ResearchConceptsG filmsDorsal root gangliaTissue engineeringExcellent adhesive propertiesComposite filmsGood adhesionGood biomaterialFilmsPC12 cellsNGF-differentiated PC12 cellsC6 glioma cellsAdhesive propertiesRoot gangliaGlial cellsCerebral tissueHippocampal neuronsNerve repairMixture of CHGlioma cellsC6 lineTissue regenerationNeural cellsLysine filmsNeuronsEngineering
2006
Gelation under dynamic conditions: A strategy for in vitro cell ordering
Doncel-Pérez E, Darder M, Martín-López E, Vázquez L, Nieto-Sampedro M, Ruiz-Hitzky E. Gelation under dynamic conditions: A strategy for in vitro cell ordering. Journal Of Materials Science: Materials In Medicine 2006, 17: 795-802. PMID: 16932860, DOI: 10.1007/s10856-006-9837-y.Peer-Reviewed Original ResearchConceptsSpin-coating conditionsAtomic force microscopyNeuronal process outgrowthLow-temperature scanning electron microscopyScanning electron microscopyBiodegradable substrateRat C6 glioma cellsFluorescence microscopyCentrifugal forceCell orderingDynamic conditionsC6 glioma cellsForce microscopyProcess outgrowthAxon growthFibrin hydrogelsUniform distributionCell modelGlioma cellsC6 cellsElectron microscopy