2008
Mammalian mitochondria have the innate ability to import tRNAs by a mechanism distinct from protein import
Rubio MA, Rinehart JJ, Krett B, Duvezin-Caubet S, Reichert AS, Söll D, Alfonzo JD. Mammalian mitochondria have the innate ability to import tRNAs by a mechanism distinct from protein import. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 9186-9191. PMID: 18587046, PMCID: PMC2453747, DOI: 10.1073/pnas.0804283105.Peer-Reviewed Original ResearchConceptsProtein importMammalian mitochondriaImport systemSubcellular RNA fractionsMitochondrial tRNA genesMitochondrial electrochemical gradientMitochondrial genomeTRNA genesTranscribed tRNAsHuman mitochondriaDefective mitochondriaProtein factorsFiber cellsHeterologous RNATRNACytosolic factorsSufficient ATPRNA fractionHuman cellsHuman diseasesProtein synthesisMitochondriaElectrochemical gradientOligonucleotide primersVitro system
1985
Transcription of a Drosophila tRNAArg gene in yeast extract: 5′-flanking sequence dependence for transcription in a heterologous system
Schaack J, Söll D. Transcription of a Drosophila tRNAArg gene in yeast extract: 5′-flanking sequence dependence for transcription in a heterologous system. Nucleic Acids Research 1985, 13: 2803-2814. PMID: 3889849, PMCID: PMC341195, DOI: 10.1093/nar/13.8.2803.Peer-Reviewed Original ResearchConceptsDrosophila Kc cell extractS. cerevisiae extractTRNA genesCerevisiae extractDrosophila tRNAArg geneCell extractsDrosophila tRNA genesDrosophila Kc cellsTRNAArg geneTranscription extractActive transcriptionTranscription kineticsExtracts of SaccharomycesSteady-state levelsKc cellsHeterologous systemsS. cerevisiaeSequence requirementsLag phaseTranscriptionGenesHeterologous combinationsPARGPosition 21DeletionFunctional analysis of fractionated Drosophila Kc cell tRNA gene transcription components.
Burke D, Söll D. Functional analysis of fractionated Drosophila Kc cell tRNA gene transcription components. Journal Of Biological Chemistry 1985, 260: 816-823. PMID: 3844013, DOI: 10.1016/s0021-9258(20)71171-3.Peer-Reviewed Original ResearchConceptsTranscription componentsTRNA genesDrosophila Kc cell extractFunctional analysisActive transcription complexesHuman HeLa cellsFactor BDrosophila systemTranscription initiationStable complex formationTranscription complexC associatesLarge complexesReconstitution experimentsCell extractsHeLa cellsCell factorFactor C.Factor CGenesStable complexesComplex formationPartial purificationComplexesDNA
1984
Transcription factor binding is limited by the 5'-flanking regions of a Drosophila tRNAHis gene and a tRNAHis pseudogene.
Cooley L, Schaack J, Burke DJ, Thomas B, Söll D. Transcription factor binding is limited by the 5'-flanking regions of a Drosophila tRNAHis gene and a tRNAHis pseudogene. Molecular And Cellular Biology 1984, 4: 2714-2722. PMID: 6570190, PMCID: PMC369281, DOI: 10.1128/mcb.4.12.2714.Peer-Reviewed Original ResearchConceptsDrosophila Kc cell extractHeLa cell extractsCell extractsReal genesStable complex formationControl regionDeletion analysisStable transcription complex formationRecombinant clonesDrosophila tRNAArg geneTRNA gene clusterTranscription complex formationBona fide genesInternal control regionTranscription factor bindingSame DNA strandComplex formationTranscription control regionsConsecutive base pairsTRNAHis geneTRNAArg geneFide genesGene clusterTranscription factorsFactor bindingThe extent of a eukaryotic tRNA gene. 5‘- and 3‘-flanking sequence dependence for transcription and stable complex formation.
Schaack J, Sharp S, Dingermann T, Burke DJ, Cooley L, Söll D. The extent of a eukaryotic tRNA gene. 5‘- and 3‘-flanking sequence dependence for transcription and stable complex formation. Journal Of Biological Chemistry 1984, 259: 1461-1467. PMID: 6693417, DOI: 10.1016/s0021-9258(17)43429-6.Peer-Reviewed Original ResearchConceptsStable complex formationBase pairsDrosophila Kc cell extractSequence requirementsCell extractsEukaryotic tRNA genesStable transcription complexesHeLa cell extractsTRNA genesComplex formationTranscription complexArg genesEfficient transcriptionTranscription assaysTranscription propertiesCell-free extractsTranscriptionHomologous systemGenesSequenceSequence dependenceCellular sourceExtractAssaysPairs
1982
The 5- flanking sequences of Drosophila tRNAArg genes control their in vitro transcription in a Drosophila cell extract.
Dingermann T, Burke D, Sharp S, Schaack J, Söll D. The 5- flanking sequences of Drosophila tRNAArg genes control their in vitro transcription in a Drosophila cell extract. Journal Of Biological Chemistry 1982, 257: 14738-14744. PMID: 6924656, DOI: 10.1016/s0021-9258(18)33342-8.Peer-Reviewed Original ResearchConceptsDrosophila tRNAArg geneTRNAArg geneDrosophila Kc cell extractFlanking sequencesTranscription efficiencyCell extractsDrosophila cell extractsEfficiency of transcriptionRegion 42ATranscriptional componentsGene clusterDrosophila extractsEfficient transcriptionTranscription factorsChromosome 2HeLa extractsTranscriptionGenesHeLa cellsSpecific sequencesHomologous extractsSequenceArgDeletionExtractThe minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription
Sharp S, Dingermann T, Söll D. The minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription. Nucleic Acids Research 1982, 10: 5393-5406. PMID: 6924209, PMCID: PMC320884, DOI: 10.1093/nar/10.18.5393.Peer-Reviewed Original ResearchConceptsD-control regionTRNA genesIntragenic control regionTranscription systemControl regionTRNAArg geneRNA synthesisGene transcriptionEukaryotic tRNA gene transcriptionHeLa cell transcription systemDrosophila tRNAArg geneT-control regionMutant tRNA genesEukaryotic tRNA genesTRNA gene transcriptionRNA polymerase IIIWild-type geneAdjacent flanking sequencesHeLa systemsMature tRNAIntragenic regionsSpecific transcriptionPolymerase IIIXenopus laevis oocytesDNA sequencesEukaryotic tRNA gene transcription is controlled by signals within and outside the mature coding sequence.
DeFranco D, Dingermann T, Johnson D, Sharp S, Söll D. Eukaryotic tRNA gene transcription is controlled by signals within and outside the mature coding sequence. Princess Takamatsu Symposia 1982, 12: 63-72. PMID: 7166550.Peer-Reviewed Original ResearchConceptsTRNA genesMature coding sequenceTranscriptional repressionTRNA sequencesControl regionCoding sequenceEukaryotic tRNA gene transcriptionDrosophila tRNAArg geneSecond control regionEukaryotic tRNA genesDrosophila tRNA genesTRNA gene transcriptionMature tRNA sequencesInitiation of transcriptionNuclease Bal 31TRNAArg geneMature tRNAPoor transcriptional activityDeletion analysisGene transcriptionNucleotides 8D-loopTranscriptional activityPromoter regionBAL-31
1981
Internal control regions for transcription of eukaryotic tRNA genes.
Sharp S, DeFranco D, Dingermann T, Farrell P, Söll D. Internal control regions for transcription of eukaryotic tRNA genes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 6657-6661. PMID: 6947245, PMCID: PMC349108, DOI: 10.1073/pnas.78.11.6657.Peer-Reviewed Original Research