2001
Post-transcriptional modification in archaeal tRNAs: identities and phylogenetic relations of nucleotides from mesophilic and hyperthermophilic Methanococcales
McCloskey J, Graham D, Zhou S, Crain P, Ibba M, Konisky J, Söll D, Olsen G. Post-transcriptional modification in archaeal tRNAs: identities and phylogenetic relations of nucleotides from mesophilic and hyperthermophilic Methanococcales. Nucleic Acids Research 2001, 29: 4699-4706. PMID: 11713320, PMCID: PMC92529, DOI: 10.1093/nar/29.22.4699.Peer-Reviewed Original ResearchMeSH KeywordsChromatography, High Pressure LiquidMethanococcalesNucleosidesNucleotidesPhylogenyRNA Processing, Post-TranscriptionalRNA, ArchaealRNA, TransferSpectrometry, Mass, Electrospray IonizationConceptsPost-transcriptional modificationsSmall ribosomal subunit RNA sequencesRibose-methylated nucleosidesClose phylogenetic relationshipArchaeal RNAArchaeal tRNAsPhylogenetic relationshipsMethanococcus jannaschiiMethanococcus maripaludisTransfer RNAPhylogenetic relationsBacterial tRNAsMethanococcus vannieliiPosition 37Methanococcus igneusModification differencesModification patternsTRNAMethanococcus thermolithotrophicusRNA sequencesRNATemperature of growthUnknown structureFamily membersEukarya
1993
SPL1-1, a Saccharomyces cerevisiae mutation affecting tRNA splicing
Kolman C, Söll D. SPL1-1, a Saccharomyces cerevisiae mutation affecting tRNA splicing. Journal Of Bacteriology 1993, 175: 1433-1442. PMID: 8444805, PMCID: PMC193230, DOI: 10.1128/jb.175.5.1433-1442.1993.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceBlotting, NorthernIntronsMolecular Sequence DataMutationNucleic Acid ConformationRNA Processing, Post-TranscriptionalRNA SplicingRNA, FungalRNA, TransferSaccharomyces cerevisiaeSequence Homology, Amino AcidSequence Homology, Nucleic AcidTranscription, GeneticConceptsTRNA genesSaccharomyces cerevisiae genesMature suppressor tRNASuppressor tRNA geneOpen reading frameSaccharomyces cerevisiae mutationsCerevisiae genesTRNA splicingSuppression phenotypeTRNA processingChromosome IIIGenetic approachesSuppressor tRNAReading frameGenetic analysisNorthern analysisMutant selectionMutantsNonsense mutationGenesMutationsLEU2Cell levelIncreased synthesisNFS1
1990
Yeast suppressor mutations and transfer RNA processing
Nichols M, Willis I, Söll D. Yeast suppressor mutations and transfer RNA processing. Methods In Enzymology 1990, 181: 377-394. PMID: 2199758, DOI: 10.1016/0076-6879(90)81137-j.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBlotting, NorthernChromosomes, FungalGenes, FungalIndicators and ReagentsMolecular Sequence DataMutationNucleic Acid ConformationNucleic Acid HybridizationRNA Polymerase IIIRNA Processing, Post-TranscriptionalRNA, TransferRNA, Transfer, SerSaccharomyces cerevisiaeSuppression, GeneticTranscription FactorsTranscription, GeneticConceptsTRNA genesMature-sized tRNAsRNA processing reactionsPrimer-directed mutagenesisAminoacyl-tRNA synthetaseTransfer RNA moleculesCognate aminoacyl-tRNA synthetaseRNA processingSuppressor mutationsTRNA locusElongation factorProtein biosynthesisRibosomal interactionsRNA moleculesMutant strainStructural proteinsPink coloniesTranscription efficiencyProcessing reactionsProtein synthesisSuppressor functionTRNALow template concentrationsGenesLoci
1988
The unusually long amino acid acceptor stem of Escherichia coli selenocysteine tRNA results from abnormal cleavage by RNase P
Burkard U, Söll D. The unusually long amino acid acceptor stem of Escherichia coli selenocysteine tRNA results from abnormal cleavage by RNase P. Nucleic Acids Research 1988, 16: 11617-11624. PMID: 3062578, PMCID: PMC339093, DOI: 10.1093/nar/16.24.11617.Peer-Reviewed Original ResearchYeast RNase P: catalytic activity and substrate binding are separate functions.
Nichols M, Söll D, Willis I. Yeast RNase P: catalytic activity and substrate binding are separate functions. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 1379-1383. PMID: 3278310, PMCID: PMC279774, DOI: 10.1073/pnas.85.5.1379.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCatalysisEndoribonucleasesProtein BindingRibonuclease PRNA Processing, Post-TranscriptionalRNA, TransferSaccharomyces cerevisiaeSchizosaccharomycesStructure-Activity RelationshipSubstrate SpecificityConceptsPrecursor tRNAsRNase PSubstrate bindingGel retardationCatalytic functionRibonucleoprotein RNase PDistinct sequence preferencesEnzyme catalytic functionRNase P cleavage siteMature tRNARNase P.Catalytic integrityTRNA precursorsRNA moietyRNA componentSequence preferenceTRNATRNA complexProtein componentsAcceptor stemEnzyme mechanismMaximal cleavageSecond nucleotideCleavage siteEnzyme
1986
The additional guanylate at the 5' terminus of Escherichia coli tRNAHis is the result of unusual processing by RNase P.
Orellana O, Cooley L, Söll D. The additional guanylate at the 5' terminus of Escherichia coli tRNAHis is the result of unusual processing by RNase P. Molecular And Cellular Biology 1986, 6: 525-529. PMID: 3023854, PMCID: PMC367542, DOI: 10.1128/mcb.6.2.525.Peer-Reviewed Original Research
1985
Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe.
Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D. Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe. Molecular And Cellular Biology 1985, 5: 808-815. PMID: 3921825, PMCID: PMC366785, DOI: 10.1128/mcb.5.4.808.Peer-Reviewed Original ResearchConceptsTRNA genesSchizosaccharomyces pombeGenomic clone bankEucaryotic tRNA genesTranscription control regionsIdentification of mutationsClone bankTRNA precursorsControl regionNonsense codonGenetic evidenceNonsense suppressorsRevertant allelesTranscriptional efficiencySaccharomyces cerevisiae extractSequence analysisSuppressor locusColony hybridizationMutational hotspotsPoint mutationsCerevisiae extractGenesPombeMutationsSplicingProcessing of precursor tRNAs in Drosophila. Processing of the 3‘ end involves an endonucleolytic cleavage and occurs after 5‘ end maturation.
Frendewey D, Dingermann T, Cooley L, Söll D. Processing of precursor tRNAs in Drosophila. Processing of the 3‘ end involves an endonucleolytic cleavage and occurs after 5‘ end maturation. Journal Of Biological Chemistry 1985, 260: 449-454. PMID: 3843841, DOI: 10.1016/s0021-9258(18)89752-6.Peer-Reviewed Original Research
1984
Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.
Willis I, Hottinger H, Pearson D, Chisholm V, Leupold U, Söll D. Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor. The EMBO Journal 1984, 3: 1573-1580. PMID: 6430697, PMCID: PMC557561, DOI: 10.1002/j.1460-2075.1984.tb02013.x.Peer-Reviewed Original ResearchMeSH KeywordsAscomycotaBase SequenceGenesMutationNucleic Acid ConformationNucleic Acid PrecursorsRNA Processing, Post-TranscriptionalRNA, TransferSchizosaccharomycesSerineConceptsTRNA precursorsDimeric tRNA precursorSerine tRNA geneEfficiency of splicingPrecursor tRNA processingSingle base changeTRNA genesTRNASer geneTRNA processingGene transcriptionNucleotide sequenceUGA mutationsD-loopMutant geneGenesBase changesExtra armMutationsIntronsTranscriptionVivo systemDimeric precursorSequenceTRNASerSplicing
1982
Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA.
Cooley L, Appel B, Söll D. Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 6475-6479. PMID: 6292903, PMCID: PMC347149, DOI: 10.1073/pnas.79.21.6475.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceDrosophila melanogasterGuanosine MonophosphateHistidineRNA Processing, Post-TranscriptionalRNA, TransferSchizosaccharomycesTranscription, GeneticConceptsMature tRNAHistidine tRNAPrimary transcriptHistidine tRNA genesGuanylate residuePost-transcriptional additionDrosophila Kc cellsTRNA genesDrosophila melanogasterSchizosaccharomyces pombeTRNAs resultsRNA speciesRNase PEukaryotic mRNAsKc cellsRNA precursorsTRNASequence analysisNucleotide additionAdditional nucleotidesPhosphodiester bondGenesNucleotidesMaturation schemeTranscripts