1985
Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe.
Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D. Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe. Molecular And Cellular Biology 1985, 5: 808-815. PMID: 3921825, PMCID: PMC366785, DOI: 10.1128/mcb.5.4.808.Peer-Reviewed Original ResearchConceptsTRNA genesSchizosaccharomyces pombeGenomic clone bankEucaryotic tRNA genesTranscription control regionsIdentification of mutationsClone bankTRNA precursorsControl regionNonsense codonGenetic evidenceNonsense suppressorsRevertant allelesTranscriptional efficiencySaccharomyces cerevisiae extractSequence analysisSuppressor locusColony hybridizationMutational hotspotsPoint mutationsCerevisiae extractGenesPombeMutationsSplicingMutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe
Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D. Mutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe. Molecular And Cellular Biology 1985, 5: 808-815. DOI: 10.1128/mcb.5.4.808-815.1985.Peer-Reviewed Original ResearchTRNA genesGenomic clone bankEucaryotic tRNA genesTranscription control regionsIdentification of mutationsSchizosaccharomyces pombeClone bankTRNA precursorsControl regionNonsense codonGenetic evidenceNonsense suppressorsRevertant allelesTranscriptional efficiencySaccharomyces cerevisiae extractSequence analysisSuppressor locusColony hybridizationMutational hotspotsPoint mutationsCerevisiae extractGenesMutationsSup3Schizosaccharomyces
1984
Transcription factor binding is limited by the 5'-flanking regions of a Drosophila tRNAHis gene and a tRNAHis pseudogene.
Cooley L, Schaack J, Burke DJ, Thomas B, Söll D. Transcription factor binding is limited by the 5'-flanking regions of a Drosophila tRNAHis gene and a tRNAHis pseudogene. Molecular And Cellular Biology 1984, 4: 2714-2722. PMID: 6570190, PMCID: PMC369281, DOI: 10.1128/mcb.4.12.2714.Peer-Reviewed Original ResearchConceptsDrosophila Kc cell extractHeLa cell extractsCell extractsReal genesStable complex formationControl regionDeletion analysisStable transcription complex formationRecombinant clonesDrosophila tRNAArg geneTRNA gene clusterTranscription complex formationBona fide genesInternal control regionTranscription factor bindingSame DNA strandComplex formationTranscription control regionsConsecutive base pairsTRNAHis geneTRNAArg geneFide genesGene clusterTranscription factorsFactor bindingTranscription Factor Binding Is Limited by the 5′-Flanking Regions of a Drosophila tRNAHis Gene and a tRNAHis Pseudogene
Cooley L, Schaack J, Burke D, Thomas B, Söll D. Transcription Factor Binding Is Limited by the 5′-Flanking Regions of a Drosophila tRNAHis Gene and a tRNAHis Pseudogene. Molecular And Cellular Biology 1984, 4: 2714-2722. DOI: 10.1128/mcb.4.12.2714-2722.1984.Peer-Reviewed Original ResearchDrosophila Kc cell extractHeLa cell extractsTRNAHis geneCell extractsReal genesStable complex formationControl regionDeletion analysisStable transcription complex formationRecombinant clonesDrosophila tRNAArg geneTRNA gene clusterTranscription complex formationBona fide genesInternal control regionTranscription factor bindingSame DNA strandComplex formationTranscription control regionsConsecutive base pairsTRNAArg geneFide genesGene clusterTranscription factorsFactor binding
1980
Analysis of a drosophila tRNA gene cluster
Hovemann B, Sharp S, Yamada H, Söll D. Analysis of a drosophila tRNA gene cluster. Cell 1980, 19: 889-895. PMID: 6769590, DOI: 10.1016/0092-8674(80)90080-x.Peer-Reviewed Original ResearchConceptsTRNA genesFlanking regionsTRNA gene clusterDrosophila tRNA genesTranscription control regionsSecondary structure featuresTranscription directionDrosophila DNAGene clusterMature tRNATRNALys geneControl regionDNA sequencesNucleotide sequenceConsensus sequenceGene regionKb fragmentTerminal sequenceGenesCol E1DNA strandsDNATRNAHybridization of DNASequence