1985
Transcription of a Drosophila tRNAArg gene in yeast extract: 5′-flanking sequence dependence for transcription in a heterologous system
Schaack J, Söll D. Transcription of a Drosophila tRNAArg gene in yeast extract: 5′-flanking sequence dependence for transcription in a heterologous system. Nucleic Acids Research 1985, 13: 2803-2814. PMID: 3889849, PMCID: PMC341195, DOI: 10.1093/nar/13.8.2803.Peer-Reviewed Original ResearchConceptsDrosophila Kc cell extractS. cerevisiae extractTRNA genesCerevisiae extractDrosophila tRNAArg geneCell extractsDrosophila tRNA genesDrosophila Kc cellsTRNAArg geneTranscription extractActive transcriptionTranscription kineticsExtracts of SaccharomycesSteady-state levelsKc cellsHeterologous systemsS. cerevisiaeSequence requirementsLag phaseTranscriptionGenesHeterologous combinationsPARGPosition 21Deletion
1984
Transcription factor binding is limited by the 5'-flanking regions of a Drosophila tRNAHis gene and a tRNAHis pseudogene.
Cooley L, Schaack J, Burke DJ, Thomas B, Söll D. Transcription factor binding is limited by the 5'-flanking regions of a Drosophila tRNAHis gene and a tRNAHis pseudogene. Molecular And Cellular Biology 1984, 4: 2714-2722. PMID: 6570190, PMCID: PMC369281, DOI: 10.1128/mcb.4.12.2714.Peer-Reviewed Original ResearchConceptsDrosophila Kc cell extractHeLa cell extractsCell extractsReal genesStable complex formationControl regionDeletion analysisStable transcription complex formationRecombinant clonesDrosophila tRNAArg geneTRNA gene clusterTranscription complex formationBona fide genesInternal control regionTranscription factor bindingSame DNA strandComplex formationTranscription control regionsConsecutive base pairsTRNAHis geneTRNAArg geneFide genesGene clusterTranscription factorsFactor bindingTranscription Factor Binding Is Limited by the 5′-Flanking Regions of a Drosophila tRNAHis Gene and a tRNAHis Pseudogene
Cooley L, Schaack J, Burke D, Thomas B, Söll D. Transcription Factor Binding Is Limited by the 5′-Flanking Regions of a Drosophila tRNAHis Gene and a tRNAHis Pseudogene. Molecular And Cellular Biology 1984, 4: 2714-2722. DOI: 10.1128/mcb.4.12.2714-2722.1984.Peer-Reviewed Original ResearchDrosophila Kc cell extractHeLa cell extractsTRNAHis geneCell extractsReal genesStable complex formationControl regionDeletion analysisStable transcription complex formationRecombinant clonesDrosophila tRNAArg geneTRNA gene clusterTranscription complex formationBona fide genesInternal control regionTranscription factor bindingSame DNA strandComplex formationTranscription control regionsConsecutive base pairsTRNAArg geneFide genesGene clusterTranscription factorsFactor binding
1983
Each element of the Drosophila tRNA Arg gene split promoter directs transcription in Xenopus oocytes
Sharp S, Dingermann T, Schaack J, Sharp J, Burke D, DeRobertis E, Söll D. Each element of the Drosophila tRNA Arg gene split promoter directs transcription in Xenopus oocytes. Nucleic Acids Research 1983, 11: 8677-8690. PMID: 6561520, PMCID: PMC326616, DOI: 10.1093/nar/11.24.8677.Peer-Reviewed Original ResearchConceptsD-control regionDrosophila tRNAArg geneTRNAArg geneEukaryotic tRNA gene transcriptionXenopus oocytesRNA polymerase III transcription factorT-control regionEukaryotic tRNA genesTRNA gene transcriptionIntragenic control regionTranscription initiation siteSpecific DNA sequencesTRNA genesTranscription initiationArg genesControl regionTranscription factorsGene transcriptionDNA sequencesMutant formsOocyte nucleusSequence 5Initiation siteGenesTranscriptionStable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions.
Dingermann T, Sharp S, Schaack J, Söll D. Stable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions. Journal Of Biological Chemistry 1983, 258: 10395-10402. PMID: 6309803, DOI: 10.1016/s0021-9258(17)44470-x.Peer-Reviewed Original ResearchConceptsIntragenic control regionControl regionTRNA genesTRNAArg geneDrosophila Kc cell extractStable transcription complex formationTranscription efficiencyDrosophila tRNAArg geneT-control regionMutant tRNA genesEukaryotic tRNA genesTranscription complex formationStable transcription complexesWild-type geneXhoI linkerTranscriptional roleTranscription initiationTranscription complexDNA regionsType genesTranscription factorsGene transcriptionTermination sitesDNA fragmentsCell extractsTranscription of eukaryotic tRNA genes in vitro. I. Analysis of control regions using a competition assay.
Sharp S, Dingermann T, Schaack J, DeFranco D, Söll D. Transcription of eukaryotic tRNA genes in vitro. I. Analysis of control regions using a competition assay. Journal Of Biological Chemistry 1983, 258: 2440-2446. PMID: 6549757, DOI: 10.1016/s0021-9258(18)32945-4.Peer-Reviewed Original ResearchConceptsT-control regionD-control regionTRNA gene transcriptionTRNAArg geneControl regionTranscription factorsCompetitive abilityDeletion mutantsGene transcriptionEukaryotic tRNA gene transcriptionDrosophila tRNAArg geneEukaryotic tRNA genesStem regionIntragenic control regionEfficiency of transcriptionTRNA genesTranscription extractTRNA productD-loopTranscription levelsWild typeTranscriptionT-loopGenesCompetition assaysTranscription of eukaryotic tRNA genes in vitro. II. Formation of stable complexes.
Schaack J, Sharp S, Dingermann T, Söll D. Transcription of eukaryotic tRNA genes in vitro. II. Formation of stable complexes. Journal Of Biological Chemistry 1983, 258: 2447-2453. PMID: 6549758, DOI: 10.1016/s0021-9258(18)32946-6.Peer-Reviewed Original ResearchConceptsStable transcription complex formationTRNA genesTranscription complex formationStable transcription complexesTranscription complexDrosophila Kc cell extractGene regionD-stemDrosophila tRNAArg geneEukaryotic tRNA genesDrosophila tRNA genesTranscription termination sequenceTRNAArg geneStable complexesComplex formationTranscription experimentsDNA regionsTranscription factorsFactor bindingCell-free extractsTermination sequenceSequence 5T-stemCell extractsDeletion mutations
1982
The 5- flanking sequences of Drosophila tRNAArg genes control their in vitro transcription in a Drosophila cell extract.
Dingermann T, Burke D, Sharp S, Schaack J, Söll D. The 5- flanking sequences of Drosophila tRNAArg genes control their in vitro transcription in a Drosophila cell extract. Journal Of Biological Chemistry 1982, 257: 14738-14744. PMID: 6924656, DOI: 10.1016/s0021-9258(18)33342-8.Peer-Reviewed Original ResearchConceptsDrosophila tRNAArg geneTRNAArg geneDrosophila Kc cell extractFlanking sequencesTranscription efficiencyCell extractsDrosophila cell extractsEfficiency of transcriptionRegion 42ATranscriptional componentsGene clusterDrosophila extractsEfficient transcriptionTranscription factorsChromosome 2HeLa extractsTranscriptionGenesHeLa cellsSpecific sequencesHomologous extractsSequenceArgDeletionExtractThe minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription
Sharp S, Dingermann T, Söll D. The minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription. Nucleic Acids Research 1982, 10: 5393-5406. PMID: 6924209, PMCID: PMC320884, DOI: 10.1093/nar/10.18.5393.Peer-Reviewed Original ResearchConceptsD-control regionTRNA genesIntragenic control regionTranscription systemControl regionTRNAArg geneRNA synthesisGene transcriptionEukaryotic tRNA gene transcriptionHeLa cell transcription systemDrosophila tRNAArg geneT-control regionMutant tRNA genesEukaryotic tRNA genesTRNA gene transcriptionRNA polymerase IIIWild-type geneAdjacent flanking sequencesHeLa systemsMature tRNAIntragenic regionsSpecific transcriptionPolymerase IIIXenopus laevis oocytesDNA sequencesEukaryotic tRNA gene transcription is controlled by signals within and outside the mature coding sequence.
DeFranco D, Dingermann T, Johnson D, Sharp S, Söll D. Eukaryotic tRNA gene transcription is controlled by signals within and outside the mature coding sequence. Princess Takamatsu Symposia 1982, 12: 63-72. PMID: 7166550.Peer-Reviewed Original ResearchConceptsTRNA genesMature coding sequenceTranscriptional repressionTRNA sequencesControl regionCoding sequenceEukaryotic tRNA gene transcriptionDrosophila tRNAArg geneSecond control regionEukaryotic tRNA genesDrosophila tRNA genesTRNA gene transcriptionMature tRNA sequencesInitiation of transcriptionNuclease Bal 31TRNAArg geneMature tRNAPoor transcriptional activityDeletion analysisGene transcriptionNucleotides 8D-loopTranscriptional activityPromoter regionBAL-31
1979
The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts.
Silverman S, Schmidt O, Söll D, Hovemann B. The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts. Journal Of Biological Chemistry 1979, 254: 10290-10294. PMID: 114522, DOI: 10.1016/s0021-9258(19)86707-8.Peer-Reviewed Original ResearchConceptsMature tRNAPrecursor RNANucleotide sequenceDrosophila tRNAArg geneGerminal vesicle extractsArginine tRNA geneXenopus germinal vesicleTRNA genesTRNAArg genePrimary transcriptDNA sequencesLeader sequenceVesicle extractsT residuesGenesTranscriptionGerminal vesicleTerminusVitro systemTRNAA sequenceRNASequenceNucleotidesTRNAArg