1997
α-Latrotoxin Stimulates Exocytosis by the Interaction with a Neuronal G-Protein-Coupled Receptor
Krasnoperov V, Bittner M, Beavis R, Kuang Y, Salnikow K, Chepurny O, Little A, Plotnikov A, Wu D, Holz R, Petrenko A. α-Latrotoxin Stimulates Exocytosis by the Interaction with a Neuronal G-Protein-Coupled Receptor. Neuron 1997, 18: 925-937. PMID: 9208860, DOI: 10.1016/s0896-6273(00)80332-3.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBrain ChemistryCalciumCattleChromaffin GranulesCloning, MolecularCOS CellsDimerizationExocytosisGTP-Binding ProteinsMembrane ProteinsMolecular Sequence DataProtein PrecursorsQa-SNARE ProteinsRatsReceptors, Cell SurfaceReceptors, PeptideRecombinant ProteinsSensory Receptor CellsSequence AlignmentSequence Homology, Amino AcidSignal TransductionSpider VenomsTissue DistributionConceptsG protein-coupled receptorsAlpha-latrotoxin receptorNeuronal G protein-coupled receptorNovel orphan G-protein-coupled receptorI alphaCalcium-independent receptorSecretin receptor familyOrphan G protein-coupled receptorRegulation of neurosecretionEndogenous proteolytic cleavageMolecular cloningPrecursor polypeptideSerpentine receptorsCIRLFusion complexExtracellular bindingAlpha-LatrotoxinFunctional expressionReceptor familyProteolytic cleavagePresynaptic receptorsAffinity columnNeurotransmitter releaseStable complexesPresynaptic neurotoxins
1996
Selective G Protein Coupling by C-C Chemokine Receptors (∗)
Kuang Y, Wu Y, Jiang H, Wu D. Selective G Protein Coupling by C-C Chemokine Receptors (∗). Journal Of Biological Chemistry 1996, 271: 3975-3978. PMID: 8626727, DOI: 10.1074/jbc.271.8.3975.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAvian ProteinsCell LineChemokine CCL2Chlorocebus aethiopsCloning, MolecularDNA PrimersDNA-Binding ProteinsGTP-Binding ProteinsHumansKineticsMonocytesPertussis ToxinPolymerase Chain ReactionReceptors, CCR2Receptors, ChemokineReceptors, CytokineRecombinant ProteinsTransfectionType C PhospholipasesVirulence Factors, BordetellaZinc FingersConceptsC chemokine receptor 1C chemokine receptorChemokine receptorsBroad spectrum anti-inflammatory drugsPertussis toxin-sensitive mannerChemokine receptor 1MCP-1 receptorAnti-inflammatory drugsG protein coupling specificityToxin-sensitive mannerGq classG protein couplingAccumulation of inositolPhospholipase CC chemokinesT cellsInflammatory reactionCotransfected COS-7 cellsGi proteinsReceptor 1Major mediatorC-terminal intracellular domainG beta gammaProtein couplingReceptors
1993
Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins.
Wu D, Jiang H, Katz A, Simon M. Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins. Journal Of Biological Chemistry 1993, 268: 3704-3709. PMID: 8381437, DOI: 10.1016/s0021-9258(18)53750-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarbacholCell LineCloning, MolecularEnzyme ActivationGTP-Binding ProteinsInositol PhosphatesKineticsMolecular Sequence DataMutagenesisNorepinephrinePhosphatidylinositol Diacylglycerol-LyasePhosphoric Diester HydrolasesPlasmidsReceptors, MuscarinicRecombinant ProteinsRestriction MappingSequence DeletionTransfectionConceptsG alpha q.PLC-beta 1COS-7 cellsG alpha qAlpha q.Alpha subunitG protein alpha subunitsAlpha qMuscarinic acetylcholine receptor type 1G alpha subunitsPhosphoinositide-specific phospholipase CIntrinsic enzymatic activitySpecific deletionAmino acid sequenceLigand-induced releasePhospholipase C beta 1C beta 1CDNA clonesAcid sequenceC-terminusG proteinsTerminal endDependent activationPhospholipase CAlpha 1C-adrenergic receptor