2021
α-Fetoprotein mRNA in situ hybridisation is a highly specific marker of hepatocellular carcinoma: a multi-centre study
Lu SX, Huang YH, Liu LL, Zhang CZ, Yang X, Yang YZ, Shao CK, Li JM, Xie D, Zhang X, Jain D, Yun JP. α-Fetoprotein mRNA in situ hybridisation is a highly specific marker of hepatocellular carcinoma: a multi-centre study. British Journal Of Cancer 2021, 124: 1988-1996. PMID: 33824478, PMCID: PMC8184895, DOI: 10.1038/s41416-021-01363-4.Peer-Reviewed Original ResearchConceptsHepatocellular carcinomaHepatocytic lesionsDiagnosis of HCCLiver biopsy cohortHepatocyte paraffin 1Independent retrospective cohortsBenign liver lesionsMulti-center studyGood diagnostic valueNovel diagnostic biomarkersArginase-1 (Arg-1) proteinsΑ-fetoprotein mRNASurgical cohortRetrospective cohortBiopsy cohortHepPar-1Arg-1Liver lesionsDiagnostic biomarkersDiagnostic valueMalignancy lesionsLesionsGlypican3CohortSpecific markers
2004
Ectonucleotidase NTPDase2 Is Selectively Down-Regulated in Biliary Cirrhosis
Dranoff JA, Kruglov EA, Toure J, Braun N, Zimmermann H, Jain D, Knowles AF, Sévigny J. Ectonucleotidase NTPDase2 Is Selectively Down-Regulated in Biliary Cirrhosis. Journal Of Investigative Medicine 2004, 52: 475. PMID: 15651265, DOI: 10.1136/jim-52-07-42.Peer-Reviewed Original ResearchConceptsBile duct ligationNTPDase2 expressionPrimary biliary cirrhosisBiliary cirrhosisPortal fibroblastsReal-time polymerase chain reactionCCl4 administrationPolymerase chain reactionBiopsy specimensPortal areasExperimental ratsFibrous bandsNormal liverConfocal immunofluorescenceAlpha-smooth muscle actinExpression of NTPDase2Fibrogenic liver cellsHepatitis C cirrhosisLiver biopsy specimensFibrotic liver diseaseHuman liver biopsy specimensChain reactionNew therapeutic approachesHepatic stellate cellsCarbon tetrachloride administration
2002
Isolation of Primary Rat Liver Fibroblasts
Kruglov EA, Jain D, Dranoff JA. Isolation of Primary Rat Liver Fibroblasts. Journal Of Investigative Medicine 2002, 50: 179. PMID: 12033282, DOI: 10.2310/6650.2002.33431.Peer-Reviewed Original ResearchConceptsLiver fibroblastsProcollagen-1 mRNAReverse transcription-polymerase chain reactionRat liverTranscription-polymerase chain reactionDistinct liver cell populationsSmooth muscle actinSmooth muscle cellsStandard cell culture methodsLiver cell populationsRole of fibroblastsVon Willebrand factorPolymerase chain reactionStellate cellsProcollagen 1Muscle actinCell markersMuscle cellsLiver physiologyAppearance of cellsWillebrand factorFibroblast morphologyChain reactionLiver researchCell populations