2002
Production of Antibodies that Recognize Specific Tyrosine‐Phosphorylated Peptides
DiGiovanna MP, Stern DF, Roussel RR. Production of Antibodies that Recognize Specific Tyrosine‐Phosphorylated Peptides. Current Protocols In Immunology 2002, 50: 11.6.1-11.6.19. PMID: 18432869, DOI: 10.1002/0471142735.im0101s50.Books
2000
Production of Antibodies That Recognize Specific Tyrosine‐Phosphorylated Peptides
DiGiovanna M, Roussel R, Stern D. Production of Antibodies That Recognize Specific Tyrosine‐Phosphorylated Peptides. Current Protocols In Molecular Biology 2000, 50: 18.6.1-18.6.19. PMID: 18265171, DOI: 10.1002/0471142727.mb1806s50.BooksConceptsAnti-phosphopeptide antibodiesIndividual phosphorylation sitesAnalysis of phosphoproteinsAnti-phosphoamino acid antibodiesPhosphorylation sitesUnphosphorylated proteinTyrosine phosphorylated peptidesCognate proteinPhosphorylated stateParticular proteinAffinity matrixProteinUnique specificityPhosphoproteinFunctional stateDifferential isolationSuch reagentsPhosphotyrosinePeptidesSupport protocolSpeciesAbundanceProduction of antibodiesConventional antibodies