1994
Analysis of Egr-1 protein induction in murine peritoneal macrophages treated with granulocyte-macrophage colony-stimulating factor.
Li C, Mitchell D, Coleman D. Analysis of Egr-1 protein induction in murine peritoneal macrophages treated with granulocyte-macrophage colony-stimulating factor. The Yale Journal Of Biology And Medicine 1994, 67: 269-76. PMID: 8579643, PMCID: PMC2588905.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, WesternCell DivisionDNA-Binding ProteinsDose-Response Relationship, DrugEarly Growth Response Protein 1Granulocyte-Macrophage Colony-Stimulating FactorImmediate-Early ProteinsMacrophages, PeritonealMiceMolecular Sequence DataThioglycolatesTranscription FactorsConceptsEgr-1 proteinMurine peritoneal macrophagesEarly growth response gene-1Macrophage proliferationGranulocyte-macrophage colony-stimulating factorResponse gene-1Egr-1 mRNAProtein speciesColony-stimulating factorGM-CSFPathogen invasionWestern blot analysisGene 1Stimulation of macrophagesMacrophage differentiationPeritoneal macrophagesProtein inductionProteinBlot analysisTranscriptionProliferationGM-CSF inductionInductionMacrophagesPhysiologic circumstances
1991
Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran
Rupprecht A, Coleman D. Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran. Journal Of Immunological Methods 1991, 144: 157-163. PMID: 1960412, DOI: 10.1016/0022-1759(91)90082-q.Peer-Reviewed Original ResearchConceptsReporter geneMurine peritoneal macrophagesBacterial chloramphenicol acetyl transferase (CAT) reporter geneChloramphenicol acetyl transferase (CAT) reporter geneAcetyl transferase reporter geneCAT reporter geneHeterologous plasmid DNAEukaryotic expression vectorReporter gene assayThioglycollate-elicited murine peritoneal macrophagesCAT activityHigher CAT activityTransfection of macrophagesGene regulationPeritoneal macrophagesPrimary culturesExpression vectorPrimary macrophage culturesGene assayTransfection strategyGenesTransfectionDEAE-dextranAdherent macrophagesPlasmid DNA
1988
Granulocyte-Macrophage Colony-Stimulating Factor Enhances Selective Effector Functions of Tissue-Derived Macrophages
Coleman D, Chodakewitz J, Bartiss A, Mellors J. Granulocyte-Macrophage Colony-Stimulating Factor Enhances Selective Effector Functions of Tissue-Derived Macrophages. Blood 1988, 72: 573-578. DOI: 10.1182/blood.v72.2.573.573.Peer-Reviewed Original ResearchFc-dependent phagocytosisAnti-Toxoplasma activityGM-CSFTissue-derived macrophagesRecombinant GM-CSFClass II major histocompatibility antigensGranulocyte-macrophage colony-stimulating factorMacrophage oxidative metabolismMajor histocompatibility antigensSite of exposureGM-CSF antibodyColony-stimulating factorMurine recombinant GM-CSFThioglycollate-elicited cellsGranulocyte-macrophage colonyPhorbol myristate acetateNeutrophil functionHistocompatibility antigensEffector functionsVariety of cellsFunctional capacityMacrophage functionThioglycollate-elicited macrophagesResident macrophagesPeritoneal macrophagesGranulocyte-macrophage colony-stimulating factor enhances selective effector functions of tissue-derived macrophages.
Coleman D, Chodakewitz J, Bartiss A, Mellors J. Granulocyte-macrophage colony-stimulating factor enhances selective effector functions of tissue-derived macrophages. Blood 1988, 72: 573-8. PMID: 3042043, DOI: 10.1182/blood.v72.2.573.bloodjournal722573.Peer-Reviewed Original ResearchConceptsFc-dependent phagocytosisAnti-Toxoplasma activityGranulocyte-macrophage colony-stimulating factorGM-CSFColony-stimulating factorTissue-derived macrophagesRecombinant GM-CSFClass II major histocompatibility antigensMacrophage oxidative metabolismMajor histocompatibility antigensSite of exposureGM-CSF antibodyMurine recombinant GM-CSFThioglycollate-elicited cellsPhorbol myristate acetateNeutrophil functionHistocompatibility antigensEffector functionsVariety of cellsFunctional capacityMacrophage functionThioglycollate-elicited macrophagesResident macrophagesPeritoneal macrophagesStimulatory effect
1987
Stimulation of macrophage H2O2 release by resident thymocytes: Effect of a soluble factor distinct from interferon-γ
Mellors J, Bartiss A, Coleman D. Stimulation of macrophage H2O2 release by resident thymocytes: Effect of a soluble factor distinct from interferon-γ. Cellular Immunology 1987, 110: 391-399. PMID: 3121188, DOI: 10.1016/0008-8749(87)90131-6.Peer-Reviewed Original ResearchConceptsT cellsResident thymocytesAntigen-specific immune responsesSoluble factorsMacrophage microbicidal mechanismsRespiratory burst capacityLow molecular weight soluble factorMacrophage oxidative metabolismNonactivated T cellsMacrophage H2O2 releaseActivated T cellsMacrophage effector functionsEnzyme-linked immunosorbentReceptor-mediated phagocytosisImmune responseIFN-gammaEffector functionsToxoplasma gondiiControl macrophagesRespiratory burstMicrobicidal mechanismsPeritoneal macrophagesInterferon activityMacrophagesBurst capacity