2024
CRISPR-Cas9-based approaches for genetic analysis and epistatic interaction studies in Coxiella burnetii.
Steiner S, Roy C. CRISPR-Cas9-based approaches for genetic analysis and epistatic interaction studies in Coxiella burnetii. MSphere 2024, e0052324. PMID: 39560384, DOI: 10.1128/msphere.00523-24.Peer-Reviewed Original ResearchIntracellular replicationHost cellsDouble mutantGenetic analysisDot/Icm type IVB secretion systemType IVB secretion systemConstruction of double mutantsLoss-of-function mutantsIntracellular replication defectsLysosome-derived vacuoleIVB secretion systemObligate intracellular bacterial pathogenRandom transposon mutagenesisTransposon insertion mutantsCRISPR-Cas9-based technologiesIntracellular bacterial pathogensLoss-of-function mutationsMammalian host cellsIntroduction of premature stop codonsPremature stop codonSilence gene expressionTargeted gene disruptionBase editing approachesEffector mutantsSecretion system
2010
Inhibition of pathogen-induced apoptosis by a Coxiella burnetii type IV effector protein
Lührmann A, Nogueira CV, Carey KL, Roy CR. Inhibition of pathogen-induced apoptosis by a Coxiella burnetii type IV effector protein. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 18997-19001. PMID: 20944063, PMCID: PMC2973885, DOI: 10.1073/pnas.1004380107.Peer-Reviewed Original ResearchConceptsPathogen-induced apoptosisEffector proteinsMammalian hostsL. pneumophila effector proteinsType IV effector proteinsHost cellsType IV secretion systemMammalian host cellsL. pneumophilaGain of functionProtein repertoireOrganelle traffickingRepeat familyDistinct hostsSecretion systemInfection strategiesProtozoan hostsApoptosis pathwayIntracellular bacteriaIntracellular replicationVirulence determinantsProteinLegionella pneumophilaApoptosisPathogens